Studies on interaction between pazufloxacin mesylate and bovine serum albumin as well as the effect of metal ions on it(甲磺酸帕珠沙星与牛血清白蛋白相互作用及共存金属离子的影响)
用荧光光谱法研究水溶液中甲磺酸帕珠沙星与牛血清白蛋白的结合反应，测定了298，308 K温度下甲磺酸帕珠沙星与牛血清白蛋白的结合常数KA（分别为7.63×104，6.25× 104 L • mol-1）和结合位点数n（分别为1.04, 1.03), 探讨了其荧光猝灭机制；根据热力学参数确定了甲磺酸帕珠沙星与牛血清白蛋白之间的作用力以静电作用为主，并运用Föster偶极-偶极非辐射能量转移原理，测定了其相互结合时授一受体间距离r为3.17 nm；采用同步荧光技术确定甲磺酸帕珠沙星对牛血清白蛋白构象产生一定的影响；进一步考察了3种金属离子对该结合反应的影响，探讨了其相互作用机制....
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2007-03-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/zjup/1008-9497.2007.34.2.205-209 |
| _version_ | 1797236223889113088 |
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| author | YOUJin-zong(游金宗) SHAOShuang(邵爽) LEIQun-fang(雷群芳) |
| author_facet | YOUJin-zong(游金宗) SHAOShuang(邵爽) LEIQun-fang(雷群芳) |
| author_sort | YOUJin-zong(游金宗) |
| collection | DOAJ |
| description | 用荧光光谱法研究水溶液中甲磺酸帕珠沙星与牛血清白蛋白的结合反应，测定了298，308 K温度下甲磺酸帕珠沙星与牛血清白蛋白的结合常数KA（分别为7.63×104，6.25× 104 L • mol-1）和结合位点数n（分别为1.04, 1.03), 探讨了其荧光猝灭机制；根据热力学参数确定了甲磺酸帕珠沙星与牛血清白蛋白之间的作用力以静电作用为主，并运用Föster偶极-偶极非辐射能量转移原理，测定了其相互结合时授一受体间距离r为3.17 nm；采用同步荧光技术确定甲磺酸帕珠沙星对牛血清白蛋白构象产生一定的影响；进一步考察了3种金属离子对该结合反应的影响，探讨了其相互作用机制. |
| first_indexed | 2024-04-24T17:00:27Z |
| format | Article |
| id | doaj.art-51078b9e762c45c48736448191bf5de4 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T17:00:27Z |
| publishDate | 2007-03-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-51078b9e762c45c48736448191bf5de42024-03-29T01:58:24ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972007-03-01342205209zjup/1008-9497.2007.34.2.205-209Studies on interaction between pazufloxacin mesylate and bovine serum albumin as well as the effect of metal ions on it(甲磺酸帕珠沙星与牛血清白蛋白相互作用及共存金属离子的影响)YOUJin-zong(游金宗)0SHAOShuang(邵爽)1LEIQun-fang(雷群芳)2 1.Department of Chemistry, Zhejiang Education Institute, Hangzhou 310012, China( 1.浙江教育学院化学系，浙江 杭州 310012) 1.Department of Chemistry, Zhejiang Education Institute, Hangzhou 310012, China( 1.浙江教育学院化学系，浙江 杭州 310012) 2.Department of Chemistry, Zhejiang University, Hangzhou 310027, China( 2.浙江大学化学系，浙江 杭州 310027)用荧光光谱法研究水溶液中甲磺酸帕珠沙星与牛血清白蛋白的结合反应，测定了298，308 K温度下甲磺酸帕珠沙星与牛血清白蛋白的结合常数KA（分别为7.63×104，6.25× 104 L • mol-1）和结合位点数n（分别为1.04, 1.03), 探讨了其荧光猝灭机制；根据热力学参数确定了甲磺酸帕珠沙星与牛血清白蛋白之间的作用力以静电作用为主，并运用Föster偶极-偶极非辐射能量转移原理，测定了其相互结合时授一受体间距离r为3.17 nm；采用同步荧光技术确定甲磺酸帕珠沙星对牛血清白蛋白构象产生一定的影响；进一步考察了3种金属离子对该结合反应的影响，探讨了其相互作用机制.https://doi.org/zjup/1008-9497.2007.34.2.205-209甲磺酸帕珠沙星牛血清白蛋白荧光猝灭同步荧光 |
| spellingShingle | YOUJin-zong(游金宗) SHAOShuang(邵爽) LEIQun-fang(雷群芳) Studies on interaction between pazufloxacin mesylate and bovine serum albumin as well as the effect of metal ions on it(甲磺酸帕珠沙星与牛血清白蛋白相互作用及共存金属离子的影响) Zhejiang Daxue xuebao. Lixue ban 甲磺酸帕珠沙星 牛血清白蛋白 荧光猝灭 同步荧光 |
| title | Studies on interaction between pazufloxacin mesylate and bovine serum albumin as well as the effect of metal ions on it(甲磺酸帕珠沙星与牛血清白蛋白相互作用及共存金属离子的影响) |
| title_full | Studies on interaction between pazufloxacin mesylate and bovine serum albumin as well as the effect of metal ions on it(甲磺酸帕珠沙星与牛血清白蛋白相互作用及共存金属离子的影响) |
| title_fullStr | Studies on interaction between pazufloxacin mesylate and bovine serum albumin as well as the effect of metal ions on it(甲磺酸帕珠沙星与牛血清白蛋白相互作用及共存金属离子的影响) |
| title_full_unstemmed | Studies on interaction between pazufloxacin mesylate and bovine serum albumin as well as the effect of metal ions on it(甲磺酸帕珠沙星与牛血清白蛋白相互作用及共存金属离子的影响) |
| title_short | Studies on interaction between pazufloxacin mesylate and bovine serum albumin as well as the effect of metal ions on it(甲磺酸帕珠沙星与牛血清白蛋白相互作用及共存金属离子的影响) |
| title_sort | studies on interaction between pazufloxacin mesylate and bovine serum albumin as well as the effect of metal ions on it 甲磺酸帕珠沙星与牛血清白蛋白相互作用及共存金属离子的影响 |
| topic | 甲磺酸帕珠沙星 牛血清白蛋白 荧光猝灭 同步荧光 |
| url | https://doi.org/zjup/1008-9497.2007.34.2.205-209 |
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