Effects of oxidative stress on endothelial modulation of contractions in aorta from renal hypertensive rats
Background: Endothelial dysfunction is linked to exaggerated production of superoxide anions. This study was conducted to examine the effects of oxidative stress on endothelial modulation of contractions in chronic two-kidney, one-clip (2K1C) renal hypertensive rats. Methods: The 2K1C hypertension w...
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| Format: | Article |
| Language: | English |
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The Korean Society of Nephrology
2014-03-01
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| Series: | Kidney Research and Clinical Practice |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211913213001162 |
| _version_ | 1819130711459758080 |
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| author | Seok Choi Hye Rang Shin Sang Hoon Kim Mi Jung Lee Jae Yeoul Jun Hyun Lee Kim Jong Hoon Chung Cheol Ho Yeum |
| author_facet | Seok Choi Hye Rang Shin Sang Hoon Kim Mi Jung Lee Jae Yeoul Jun Hyun Lee Kim Jong Hoon Chung Cheol Ho Yeum |
| author_sort | Seok Choi |
| collection | DOAJ |
| description | Background: Endothelial dysfunction is linked to exaggerated production of superoxide anions. This study was conducted to examine the effects of oxidative stress on endothelial modulation of contractions in chronic two-kidney, one-clip (2K1C) renal hypertensive rats.
Methods: The 2K1C hypertension was induced by clipping the left renal artery; age-matched rats receiving sham treatment served as controls. Thoracic aortae were isolated and mounted in tissue baths for measurement of isometric tension.
Results: Norepinephrine-induced contraction was augmented by the removal of the endothelium, which was more pronounced in sham rats than in 2K1C rats. Nω-nitro-L-arginine methyl ester, an inhibitor of nitric oxide production, had a similar augmenting effect. Vitamin C inhibited the contraction in aortic rings with intact endothelium from 2K1C rats but not from sham rats. The contraction was also suppressed by treatment with diphenyleneiodonium or apocynin, inhibitors of nicotinamide adenine dinucleotide/nicotinamide adenine dinucleotide phosphate (NADH/NADPH) oxidase, in the aortae with intact endothelium from 2K1C rats but not in those from sham rats. Superoxide anions generated by xanthine oxidase/hypoxanthine enhanced the contraction in the aortae with intact endothelium from sham rats, but had no effect in 2K1C rats. Enhanced contractile responses to norepinephrine by xanthine oxidase/hypoxanthine in sham rats were reversed by vitamin C.
Conclusion: These results suggest that the effect on endothelial modulation of endothelium-derived nitric oxide is impaired in 2K1C hypertension. The impairment is, at least in part, related to increased production of superoxide anions by NADH/NADPH oxidase. |
| first_indexed | 2024-12-22T09:03:57Z |
| format | Article |
| id | doaj.art-5107a529efeb47b1ac560b70d746dae7 |
| institution | Directory Open Access Journal |
| issn | 2211-9132 |
| language | English |
| last_indexed | 2024-12-22T09:03:57Z |
| publishDate | 2014-03-01 |
| publisher | The Korean Society of Nephrology |
| record_format | Article |
| series | Kidney Research and Clinical Practice |
| spelling | doaj.art-5107a529efeb47b1ac560b70d746dae72022-12-21T18:31:39ZengThe Korean Society of NephrologyKidney Research and Clinical Practice2211-91322014-03-01331192510.1016/j.krcp.2013.12.001Effects of oxidative stress on endothelial modulation of contractions in aorta from renal hypertensive ratsSeok Choi0Hye Rang Shin1Sang Hoon Kim2Mi Jung Lee3Jae Yeoul Jun4Hyun Lee Kim5Jong Hoon Chung6Cheol Ho Yeum7Department of Physiology, College of Medicine, Chosun University, Gwangju, KoreaDepartment of Psychiatry, College of Medicine, Chosun University, Gwangju, KoreaDepartment of Psychiatry, College of Medicine, Chosun University, Gwangju, KoreaDepartment of Physiology, College of Medicine, Chosun University, Gwangju, KoreaDepartment of Physiology, College of Medicine, Chosun University, Gwangju, KoreaDepartment of Internal Medicine, College of Medicine, Chosun University, Gwangju, KoreaDepartment of Internal Medicine, College of Medicine, Chosun University, Gwangju, KoreaDepartment of Physiology, College of Medicine, Chosun University, Gwangju, KoreaBackground: Endothelial dysfunction is linked to exaggerated production of superoxide anions. This study was conducted to examine the effects of oxidative stress on endothelial modulation of contractions in chronic two-kidney, one-clip (2K1C) renal hypertensive rats. Methods: The 2K1C hypertension was induced by clipping the left renal artery; age-matched rats receiving sham treatment served as controls. Thoracic aortae were isolated and mounted in tissue baths for measurement of isometric tension. Results: Norepinephrine-induced contraction was augmented by the removal of the endothelium, which was more pronounced in sham rats than in 2K1C rats. Nω-nitro-L-arginine methyl ester, an inhibitor of nitric oxide production, had a similar augmenting effect. Vitamin C inhibited the contraction in aortic rings with intact endothelium from 2K1C rats but not from sham rats. The contraction was also suppressed by treatment with diphenyleneiodonium or apocynin, inhibitors of nicotinamide adenine dinucleotide/nicotinamide adenine dinucleotide phosphate (NADH/NADPH) oxidase, in the aortae with intact endothelium from 2K1C rats but not in those from sham rats. Superoxide anions generated by xanthine oxidase/hypoxanthine enhanced the contraction in the aortae with intact endothelium from sham rats, but had no effect in 2K1C rats. Enhanced contractile responses to norepinephrine by xanthine oxidase/hypoxanthine in sham rats were reversed by vitamin C. Conclusion: These results suggest that the effect on endothelial modulation of endothelium-derived nitric oxide is impaired in 2K1C hypertension. The impairment is, at least in part, related to increased production of superoxide anions by NADH/NADPH oxidase.http://www.sciencedirect.com/science/article/pii/S2211913213001162EndotheliumRenal hypertensionSuperoxideOxidative stress |
| spellingShingle | Seok Choi Hye Rang Shin Sang Hoon Kim Mi Jung Lee Jae Yeoul Jun Hyun Lee Kim Jong Hoon Chung Cheol Ho Yeum Effects of oxidative stress on endothelial modulation of contractions in aorta from renal hypertensive rats Kidney Research and Clinical Practice Endothelium Renal hypertension Superoxide Oxidative stress |
| title | Effects of oxidative stress on endothelial modulation of contractions in aorta from renal hypertensive rats |
| title_full | Effects of oxidative stress on endothelial modulation of contractions in aorta from renal hypertensive rats |
| title_fullStr | Effects of oxidative stress on endothelial modulation of contractions in aorta from renal hypertensive rats |
| title_full_unstemmed | Effects of oxidative stress on endothelial modulation of contractions in aorta from renal hypertensive rats |
| title_short | Effects of oxidative stress on endothelial modulation of contractions in aorta from renal hypertensive rats |
| title_sort | effects of oxidative stress on endothelial modulation of contractions in aorta from renal hypertensive rats |
| topic | Endothelium Renal hypertension Superoxide Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S2211913213001162 |
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