Efficient Extraction of the RuBisCO Enzyme from Spinach Leaves Using Aqueous Solutions of Biocompatible Ionic Liquids
Ribulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO) is the most abundant protein on the planet, being present in plants, algae and various species of bacteria, with application in the pharmaceutical, chemical, cosmetic and food industries. However, current extraction methods of RuBisCO do not a...
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MDPI AG
2021-12-01
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| Series: | Sustainable Chemistry |
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| Online Access: | https://www.mdpi.com/2673-4079/3/1/1 |
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| author | Ana I. Valente Ana M. Ferreira Mafalda R. Almeida Aminou Mohamadou Mara G. Freire Ana P. M. Tavares |
| author_facet | Ana I. Valente Ana M. Ferreira Mafalda R. Almeida Aminou Mohamadou Mara G. Freire Ana P. M. Tavares |
| author_sort | Ana I. Valente |
| collection | DOAJ |
| description | Ribulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO) is the most abundant protein on the planet, being present in plants, algae and various species of bacteria, with application in the pharmaceutical, chemical, cosmetic and food industries. However, current extraction methods of RuBisCO do not allow high yields of extraction. Therefore, the development of an efficient and selective RuBisCOs’ extraction method is required. In this work, aqueous solutions of biocompatible ionic liquids (ILs), i.e., ILs derived from choline and analogues of glycine-betaine, were applied in the RuBisCO’s extraction from spinach leaves. Three commercial imidazolium-based ILs were also investigated for comparison purposes. To optimize RuBisCO’s extraction conditions, response surface methodology was applied. Under optimum extraction conditions, extraction yields of 10.92 and 10.57 mg of RuBisCO/g of biomass were obtained with the ILs cholinium acetate ([Ch][Ac]) and cholinium chloride ([Ch]Cl), respectively. Circular dichroism (CD) spectroscopy results show that the secondary structure of RuBisCO is better preserved in the IL solutions when compared to the commonly used extraction solvent. The obtained results indicate that cholinium-based ILs are a promising and viable alternative for the extraction of RuBisCO from vegetable biomass. |
| first_indexed | 2024-12-10T13:32:18Z |
| format | Article |
| id | doaj.art-5108582f1ead44419d4f9d7f5b6893ac |
| institution | Directory Open Access Journal |
| issn | 2673-4079 |
| language | English |
| last_indexed | 2024-12-10T13:32:18Z |
| publishDate | 2021-12-01 |
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| record_format | Article |
| series | Sustainable Chemistry |
| spelling | doaj.art-5108582f1ead44419d4f9d7f5b6893ac2022-12-22T01:46:55ZengMDPI AGSustainable Chemistry2673-40792021-12-013111810.3390/suschem3010001Efficient Extraction of the RuBisCO Enzyme from Spinach Leaves Using Aqueous Solutions of Biocompatible Ionic LiquidsAna I. Valente0Ana M. Ferreira1Mafalda R. Almeida2Aminou Mohamadou3Mara G. Freire4Ana P. M. Tavares5Chemistry Department, CICECO–Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, PortugalChemistry Department, CICECO–Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, PortugalChemistry Department, CICECO–Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, PortugalInstitut de Chimie Moléculaire de Reims (ICMR)-UMR CNRS 7312, Université de Reims Champagne-Ardenne, Moulin de la Housse, BP 1039, CEDEX 2, F-51687 Reims, FranceChemistry Department, CICECO–Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, PortugalChemistry Department, CICECO–Aveiro Institute of Materials, University of Aveiro, 3810-193 Aveiro, PortugalRibulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO) is the most abundant protein on the planet, being present in plants, algae and various species of bacteria, with application in the pharmaceutical, chemical, cosmetic and food industries. However, current extraction methods of RuBisCO do not allow high yields of extraction. Therefore, the development of an efficient and selective RuBisCOs’ extraction method is required. In this work, aqueous solutions of biocompatible ionic liquids (ILs), i.e., ILs derived from choline and analogues of glycine-betaine, were applied in the RuBisCO’s extraction from spinach leaves. Three commercial imidazolium-based ILs were also investigated for comparison purposes. To optimize RuBisCO’s extraction conditions, response surface methodology was applied. Under optimum extraction conditions, extraction yields of 10.92 and 10.57 mg of RuBisCO/g of biomass were obtained with the ILs cholinium acetate ([Ch][Ac]) and cholinium chloride ([Ch]Cl), respectively. Circular dichroism (CD) spectroscopy results show that the secondary structure of RuBisCO is better preserved in the IL solutions when compared to the commonly used extraction solvent. The obtained results indicate that cholinium-based ILs are a promising and viable alternative for the extraction of RuBisCO from vegetable biomass.https://www.mdpi.com/2673-4079/3/1/1Ribulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO)spinachsolid-liquid extractionbiocompatible ionic liquids |
| spellingShingle | Ana I. Valente Ana M. Ferreira Mafalda R. Almeida Aminou Mohamadou Mara G. Freire Ana P. M. Tavares Efficient Extraction of the RuBisCO Enzyme from Spinach Leaves Using Aqueous Solutions of Biocompatible Ionic Liquids Sustainable Chemistry Ribulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO) spinach solid-liquid extraction biocompatible ionic liquids |
| title | Efficient Extraction of the RuBisCO Enzyme from Spinach Leaves Using Aqueous Solutions of Biocompatible Ionic Liquids |
| title_full | Efficient Extraction of the RuBisCO Enzyme from Spinach Leaves Using Aqueous Solutions of Biocompatible Ionic Liquids |
| title_fullStr | Efficient Extraction of the RuBisCO Enzyme from Spinach Leaves Using Aqueous Solutions of Biocompatible Ionic Liquids |
| title_full_unstemmed | Efficient Extraction of the RuBisCO Enzyme from Spinach Leaves Using Aqueous Solutions of Biocompatible Ionic Liquids |
| title_short | Efficient Extraction of the RuBisCO Enzyme from Spinach Leaves Using Aqueous Solutions of Biocompatible Ionic Liquids |
| title_sort | efficient extraction of the rubisco enzyme from spinach leaves using aqueous solutions of biocompatible ionic liquids |
| topic | Ribulose-1,5-biphosphate carboxylase/oxygenase (RuBisCO) spinach solid-liquid extraction biocompatible ionic liquids |
| url | https://www.mdpi.com/2673-4079/3/1/1 |
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