Magnolol inhibits cancer stemness and IL-6/Stat3 signaling in oral carcinomas
Background/purpose: Cancer stem cells (CSCs) have been known to be implicated in tumorigenesis, metastasis, and drug resistance in oral squamous cell carcinomas (OSCC). In this study, we aimed to investigate whether magnolol, a polyphenolic component derived from Magnolia officinalis, exhibited the...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Journal of the Formosan Medical Association |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664621000267 |
| _version_ | 1819174165738946560 |
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| author | Chih-Yu Peng Cheng-Chia Yu Chun-Chung Huang Yi-Wen Liao Pei-Ling Hsieh Pei-Ming Chu Chuan-Hang Yu Shih-Shen Lin |
| author_facet | Chih-Yu Peng Cheng-Chia Yu Chun-Chung Huang Yi-Wen Liao Pei-Ling Hsieh Pei-Ming Chu Chuan-Hang Yu Shih-Shen Lin |
| author_sort | Chih-Yu Peng |
| collection | DOAJ |
| description | Background/purpose: Cancer stem cells (CSCs) have been known to be implicated in tumorigenesis, metastasis, and drug resistance in oral squamous cell carcinomas (OSCC). In this study, we aimed to investigate whether magnolol, a polyphenolic component derived from Magnolia officinalis, exhibited the anti-CSCs properties. Methods: The cytotoxicity of magnolol was tested using normal gingival epithelioid SG cells and sphere-forming OSCC-CSCs isolated from SAS, OECM1, and GNM cells. Secondary sphere-forming ability, the proportion of ALDH1 positive cells, Transwell migration, and invasion capacities were examined as well. The chemosensitive effects of magnolol were investigated using MTT, secondary sphere-forming, and invasion assays. Results: Magnolol exerted a higher cytotoxicity of OSCC-CSCs and cancer stemness features, including self-renewal ability, the expression CSC marker, migration, and invasion capacities were all downregulated in magnolol-treated OSCC-CSCs. Moreover, administration of magnolol potentiated the effect of cisplatin, including a decrease in cell viability, self-renewal, and invasion activities. In addition, we observed that the secretion of IL-6 and phosphorylation of Stat3 were decreased in OSCC-CSCs treated with magnolol. Conclusion: Our data suggest that magnolol is able to target CSCs and suppress the cancer stemness properties, at least in part, via IL-6/Stat3 signaling. Besides, a dietary supplement of magnolol may function as an adjunct to cisplatin treatment. |
| first_indexed | 2024-12-22T20:34:38Z |
| format | Article |
| id | doaj.art-510fb6f73be143018aca9376f727de2b |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-12-22T20:34:38Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-510fb6f73be143018aca9376f727de2b2022-12-21T18:13:29ZengElsevierJournal of the Formosan Medical Association0929-66462022-01-0112115157Magnolol inhibits cancer stemness and IL-6/Stat3 signaling in oral carcinomasChih-Yu Peng0Cheng-Chia Yu1Chun-Chung Huang2Yi-Wen Liao3Pei-Ling Hsieh4Pei-Ming Chu5Chuan-Hang Yu6Shih-Shen Lin7School of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan; Institute of Oral Sciences, Chung Shan Medical University, Taichung, TaiwanInstitute of Oral Sciences, Chung Shan Medical University, Taichung, TaiwanInstitute of Oral Sciences, Chung Shan Medical University, Taichung, Taiwan; Department of Medical Research, Chung Shan Medical University Hospital, Taichung, TaiwanDepartment of Anatomy, School of Medicine, China Medical University, Taichung, TaiwanDepartment of Anatomy, School of Medicine, China Medical University, Taichung, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan; Corresponding author. School of Dentistry, Chung Shan Medical University, No.110, Sec.1, Jianguo N. Rd., Taichung 40201, Taiwan. Fax: +886 4 24759065.School of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan; Corresponding author. School of Dentistry, Chung Shan Medical University, No.110, Sec.1, Jianguo N. Rd., Taichung 40201, Taiwan. Fax: +886 4 24759065.Background/purpose: Cancer stem cells (CSCs) have been known to be implicated in tumorigenesis, metastasis, and drug resistance in oral squamous cell carcinomas (OSCC). In this study, we aimed to investigate whether magnolol, a polyphenolic component derived from Magnolia officinalis, exhibited the anti-CSCs properties. Methods: The cytotoxicity of magnolol was tested using normal gingival epithelioid SG cells and sphere-forming OSCC-CSCs isolated from SAS, OECM1, and GNM cells. Secondary sphere-forming ability, the proportion of ALDH1 positive cells, Transwell migration, and invasion capacities were examined as well. The chemosensitive effects of magnolol were investigated using MTT, secondary sphere-forming, and invasion assays. Results: Magnolol exerted a higher cytotoxicity of OSCC-CSCs and cancer stemness features, including self-renewal ability, the expression CSC marker, migration, and invasion capacities were all downregulated in magnolol-treated OSCC-CSCs. Moreover, administration of magnolol potentiated the effect of cisplatin, including a decrease in cell viability, self-renewal, and invasion activities. In addition, we observed that the secretion of IL-6 and phosphorylation of Stat3 were decreased in OSCC-CSCs treated with magnolol. Conclusion: Our data suggest that magnolol is able to target CSCs and suppress the cancer stemness properties, at least in part, via IL-6/Stat3 signaling. Besides, a dietary supplement of magnolol may function as an adjunct to cisplatin treatment.http://www.sciencedirect.com/science/article/pii/S0929664621000267Oral squamous cell carcinomasMagnololCancer stem cells |
| spellingShingle | Chih-Yu Peng Cheng-Chia Yu Chun-Chung Huang Yi-Wen Liao Pei-Ling Hsieh Pei-Ming Chu Chuan-Hang Yu Shih-Shen Lin Magnolol inhibits cancer stemness and IL-6/Stat3 signaling in oral carcinomas Journal of the Formosan Medical Association Oral squamous cell carcinomas Magnolol Cancer stem cells |
| title | Magnolol inhibits cancer stemness and IL-6/Stat3 signaling in oral carcinomas |
| title_full | Magnolol inhibits cancer stemness and IL-6/Stat3 signaling in oral carcinomas |
| title_fullStr | Magnolol inhibits cancer stemness and IL-6/Stat3 signaling in oral carcinomas |
| title_full_unstemmed | Magnolol inhibits cancer stemness and IL-6/Stat3 signaling in oral carcinomas |
| title_short | Magnolol inhibits cancer stemness and IL-6/Stat3 signaling in oral carcinomas |
| title_sort | magnolol inhibits cancer stemness and il 6 stat3 signaling in oral carcinomas |
| topic | Oral squamous cell carcinomas Magnolol Cancer stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S0929664621000267 |
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