Effect of Selenium on the Responses Induced by Heat Stress in Plant Cell Cultures
High temperatures are a significant stress factor for plants. In fact, many biochemical reactions involved in growth and development are sensitive to temperature. In particular, heat stress (HS) represents a severe issue for plant productivity and strategies to obtain high yields under this conditio...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-08-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2223-7747/7/3/64 |
| _version_ | 1819294643999735808 |
|---|---|
| author | Massimo Malerba Raffaella Cerana |
| author_facet | Massimo Malerba Raffaella Cerana |
| author_sort | Massimo Malerba |
| collection | DOAJ |
| description | High temperatures are a significant stress factor for plants. In fact, many biochemical reactions involved in growth and development are sensitive to temperature. In particular, heat stress (HS) represents a severe issue for plant productivity and strategies to obtain high yields under this condition are important goals in agriculture. While selenium (Se) is a nutrient for humans and animals, its role as a plant micronutrient is still questioned. Se can prevent several abiotic stresses (drought, heat, UV, salinity, heavy metals), but the action mechanisms are poorly understood. Se seems to regulate reactive oxygen species (ROS) and to inhibit heavy metals transport. In addition, it has been demonstrated that Se is essential for a correct integrity of cell membranes and chloroplasts, especially the photosynthetic apparatus. Previous results showed that in tobacco (Nicotiana tabacum cv. Bright-Yellow 2) cultures HS (5 min at 50 °C) induced cell death with apoptotic features, accompanied by oxidative stress and changes in the levels of stress-related proteins. In this work we investigated the effect of Se on the responses induced by HS. The obtained results show that Se markedly reduces the effects of HS on cell vitality, cytoplasmic shrinkage, superoxide anion production, membrane lipids peroxidation, activity of caspase-3-like proteases, and the levels of some stress-related proteins (Hsp90, BiP, 14-3-3s, cytochrome c). |
| first_indexed | 2024-12-24T04:29:35Z |
| format | Article |
| id | doaj.art-5114affdfb464101acfb19b06f56aafb |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-12-24T04:29:35Z |
| publishDate | 2018-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-5114affdfb464101acfb19b06f56aafb2022-12-21T17:15:27ZengMDPI AGPlants2223-77472018-08-01736410.3390/plants7030064plants7030064Effect of Selenium on the Responses Induced by Heat Stress in Plant Cell CulturesMassimo Malerba0Raffaella Cerana1Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca, 20126 Milan, ItalyDipartimento di Scienze dell’Ambiente e della Terra, Università degli Studi di Milano-Bicocca, 20126 Milan, ItalyHigh temperatures are a significant stress factor for plants. In fact, many biochemical reactions involved in growth and development are sensitive to temperature. In particular, heat stress (HS) represents a severe issue for plant productivity and strategies to obtain high yields under this condition are important goals in agriculture. While selenium (Se) is a nutrient for humans and animals, its role as a plant micronutrient is still questioned. Se can prevent several abiotic stresses (drought, heat, UV, salinity, heavy metals), but the action mechanisms are poorly understood. Se seems to regulate reactive oxygen species (ROS) and to inhibit heavy metals transport. In addition, it has been demonstrated that Se is essential for a correct integrity of cell membranes and chloroplasts, especially the photosynthetic apparatus. Previous results showed that in tobacco (Nicotiana tabacum cv. Bright-Yellow 2) cultures HS (5 min at 50 °C) induced cell death with apoptotic features, accompanied by oxidative stress and changes in the levels of stress-related proteins. In this work we investigated the effect of Se on the responses induced by HS. The obtained results show that Se markedly reduces the effects of HS on cell vitality, cytoplasmic shrinkage, superoxide anion production, membrane lipids peroxidation, activity of caspase-3-like proteases, and the levels of some stress-related proteins (Hsp90, BiP, 14-3-3s, cytochrome c).http://www.mdpi.com/2223-7747/7/3/64cell deathheat stressplant cell culturesseleniumtobacco BY-2 |
| spellingShingle | Massimo Malerba Raffaella Cerana Effect of Selenium on the Responses Induced by Heat Stress in Plant Cell Cultures Plants cell death heat stress plant cell cultures selenium tobacco BY-2 |
| title | Effect of Selenium on the Responses Induced by Heat Stress in Plant Cell Cultures |
| title_full | Effect of Selenium on the Responses Induced by Heat Stress in Plant Cell Cultures |
| title_fullStr | Effect of Selenium on the Responses Induced by Heat Stress in Plant Cell Cultures |
| title_full_unstemmed | Effect of Selenium on the Responses Induced by Heat Stress in Plant Cell Cultures |
| title_short | Effect of Selenium on the Responses Induced by Heat Stress in Plant Cell Cultures |
| title_sort | effect of selenium on the responses induced by heat stress in plant cell cultures |
| topic | cell death heat stress plant cell cultures selenium tobacco BY-2 |
| url | http://www.mdpi.com/2223-7747/7/3/64 |
| work_keys_str_mv | AT massimomalerba effectofseleniumontheresponsesinducedbyheatstressinplantcellcultures AT raffaellacerana effectofseleniumontheresponsesinducedbyheatstressinplantcellcultures |