Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung.
Innate lymphoid cells (ILC) are a new family of innate immune cells that have emerged as important regulators of tissue homeostasis and inflammation. However, limited data are available concerning the relative abundance and characteristics of ILC in the human lung.The aim of this study was to charac...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4699688?pdf=render |
| _version_ | 1831690281205891072 |
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| author | Katrien C De Grove Sharen Provoost Fien M Verhamme Ken R Bracke Guy F Joos Tania Maes Guy G Brusselle |
| author_facet | Katrien C De Grove Sharen Provoost Fien M Verhamme Ken R Bracke Guy F Joos Tania Maes Guy G Brusselle |
| author_sort | Katrien C De Grove |
| collection | DOAJ |
| description | Innate lymphoid cells (ILC) are a new family of innate immune cells that have emerged as important regulators of tissue homeostasis and inflammation. However, limited data are available concerning the relative abundance and characteristics of ILC in the human lung.The aim of this study was to characterize and enumerate the different ILC subsets in human lung by multi-color flow cytometry.Within the CD45+ Lin- CD127+ pulmonary ILC population, we identified group 1 (ILC1), group 2 (ILC2) and group 3 (ILC3) innate lymphoid cells using specific surface markers (i.e. IL12Rβ2, CRTH2 and CD117 respectively) and key transcription factors (i.e. T-bet, GATA-3 and RORγT respectively). Based on the presence of NKp44, ILC3 were further subdivided in natural cytotoxicity receptor (NCR)+ and NCR- ILC3. In addition, we demonstrated the production of signature cytokines IFN-γ, IL-5, IL-17A, IL-22 and GM-CSF in the pulmonary ILC population. Interestingly, we observed a tendency to a higher frequency of NCR- ILC3 in lungs of patients with chronic obstructive pulmonary disease (COPD) compared with controls.We show that the three main ILC subsets are present in human lung. Importantly, the relative abundance of ILC subsets tended to change in COPD patients in comparison to control individuals. |
| first_indexed | 2024-12-20T11:07:45Z |
| format | Article |
| id | doaj.art-511789b37a194f7688c2346249d27571 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T11:07:45Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-511789b37a194f7688c2346249d275712022-12-21T19:42:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01111e014596110.1371/journal.pone.0145961Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung.Katrien C De GroveSharen ProvoostFien M VerhammeKen R BrackeGuy F JoosTania MaesGuy G BrusselleInnate lymphoid cells (ILC) are a new family of innate immune cells that have emerged as important regulators of tissue homeostasis and inflammation. However, limited data are available concerning the relative abundance and characteristics of ILC in the human lung.The aim of this study was to characterize and enumerate the different ILC subsets in human lung by multi-color flow cytometry.Within the CD45+ Lin- CD127+ pulmonary ILC population, we identified group 1 (ILC1), group 2 (ILC2) and group 3 (ILC3) innate lymphoid cells using specific surface markers (i.e. IL12Rβ2, CRTH2 and CD117 respectively) and key transcription factors (i.e. T-bet, GATA-3 and RORγT respectively). Based on the presence of NKp44, ILC3 were further subdivided in natural cytotoxicity receptor (NCR)+ and NCR- ILC3. In addition, we demonstrated the production of signature cytokines IFN-γ, IL-5, IL-17A, IL-22 and GM-CSF in the pulmonary ILC population. Interestingly, we observed a tendency to a higher frequency of NCR- ILC3 in lungs of patients with chronic obstructive pulmonary disease (COPD) compared with controls.We show that the three main ILC subsets are present in human lung. Importantly, the relative abundance of ILC subsets tended to change in COPD patients in comparison to control individuals.http://europepmc.org/articles/PMC4699688?pdf=render |
| spellingShingle | Katrien C De Grove Sharen Provoost Fien M Verhamme Ken R Bracke Guy F Joos Tania Maes Guy G Brusselle Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung. PLoS ONE |
| title | Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung. |
| title_full | Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung. |
| title_fullStr | Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung. |
| title_full_unstemmed | Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung. |
| title_short | Characterization and Quantification of Innate Lymphoid Cell Subsets in Human Lung. |
| title_sort | characterization and quantification of innate lymphoid cell subsets in human lung |
| url | http://europepmc.org/articles/PMC4699688?pdf=render |
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