Bacterial Profiles of Brain in Downer Cattle with Unknown Etiology
Downer cow can be caused by muscular paralysis, neurological damage, metabolic disorder, and/or the complication of microbial infection. However, downer cow with unknown etiology is issued because of the non-detection of its bacterial etiological agent. In this study, differences in the bacterial co...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/1/98 |
| _version_ | 1797438518495019008 |
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| author | Yeong-Jun Park Gi-Ung Kang Minsoo Jeong Vineet Singh Jongho Kim Kyunghyun Lee Eun-Jin Choi Heui-Jin Kim Seungjun Lee Sook-Young Lee Jae-Ku Oem Jae-Ho Shin |
| author_facet | Yeong-Jun Park Gi-Ung Kang Minsoo Jeong Vineet Singh Jongho Kim Kyunghyun Lee Eun-Jin Choi Heui-Jin Kim Seungjun Lee Sook-Young Lee Jae-Ku Oem Jae-Ho Shin |
| author_sort | Yeong-Jun Park |
| collection | DOAJ |
| description | Downer cow can be caused by muscular paralysis, neurological damage, metabolic disorder, and/or the complication of microbial infection. However, downer cow with unknown etiology is issued because of the non-detection of its bacterial etiological agent. In this study, differences in the bacterial community in brain tissues between downer cattle with unknown etiology and healthy slaughtered cattle are investigated. Bacterial diversity and representative genera between downer and normal cattle were significantly different (<i>p</i> < 0.05). There are significant differences in representative genera of downer and normal cattle, especially the significance, fold change, and area under the receiver operating characteristic curve score (<i>p</i> < 0.05). Furthermore, the prediction of functional genes in brain microbiota between the downer and normal cattle revealed differences in the cluster of orthologous gene categories, such as lipid transport and metabolism, secondary metabolite biosynthesis, and signal transduction (<i>p</i> < 0.05). This study revealed a significant difference in microbiota between the downer and normal cattle. Thus, we demonstrate that representative genera from downer cattle through 16S rRNA gene amplicon sequencing and microbiota analysis have the potential as candidates for bacterial etiological agents for downer cow. |
| first_indexed | 2024-03-09T11:39:07Z |
| format | Article |
| id | doaj.art-5118e80e992848609fe9cc56bcc276ac |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T11:39:07Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5118e80e992848609fe9cc56bcc276ac2023-11-30T23:36:23ZengMDPI AGMicroorganisms2076-26072022-12-011119810.3390/microorganisms11010098Bacterial Profiles of Brain in Downer Cattle with Unknown EtiologyYeong-Jun Park0Gi-Ung Kang1Minsoo Jeong2Vineet Singh3Jongho Kim4Kyunghyun Lee5Eun-Jin Choi6Heui-Jin Kim7Seungjun Lee8Sook-Young Lee9Jae-Ku Oem10Jae-Ho Shin11Department of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaAnimal and Plant Quarantine Agency, Kimcheon-si 39660, Republic of KoreaAnimal and Plant Quarantine Agency, Kimcheon-si 39660, Republic of KoreaAnimal and Plant Quarantine Agency, Kimcheon-si 39660, Republic of KoreaAnimal and Plant Quarantine Agency, Kimcheon-si 39660, Republic of KoreaDepartment of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of KoreaCollege of Veterinary Medicine, Jeonbuk National University, Iksan-si 54596, Republic of KoreaCollege of Veterinary Medicine, Jeonbuk National University, Iksan-si 54596, Republic of KoreaDepartment of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of KoreaDowner cow can be caused by muscular paralysis, neurological damage, metabolic disorder, and/or the complication of microbial infection. However, downer cow with unknown etiology is issued because of the non-detection of its bacterial etiological agent. In this study, differences in the bacterial community in brain tissues between downer cattle with unknown etiology and healthy slaughtered cattle are investigated. Bacterial diversity and representative genera between downer and normal cattle were significantly different (<i>p</i> < 0.05). There are significant differences in representative genera of downer and normal cattle, especially the significance, fold change, and area under the receiver operating characteristic curve score (<i>p</i> < 0.05). Furthermore, the prediction of functional genes in brain microbiota between the downer and normal cattle revealed differences in the cluster of orthologous gene categories, such as lipid transport and metabolism, secondary metabolite biosynthesis, and signal transduction (<i>p</i> < 0.05). This study revealed a significant difference in microbiota between the downer and normal cattle. Thus, we demonstrate that representative genera from downer cattle through 16S rRNA gene amplicon sequencing and microbiota analysis have the potential as candidates for bacterial etiological agents for downer cow.https://www.mdpi.com/2076-2607/11/1/98downer cowunknown etiologybrain microbiotanext-generation sequencing |
| spellingShingle | Yeong-Jun Park Gi-Ung Kang Minsoo Jeong Vineet Singh Jongho Kim Kyunghyun Lee Eun-Jin Choi Heui-Jin Kim Seungjun Lee Sook-Young Lee Jae-Ku Oem Jae-Ho Shin Bacterial Profiles of Brain in Downer Cattle with Unknown Etiology Microorganisms downer cow unknown etiology brain microbiota next-generation sequencing |
| title | Bacterial Profiles of Brain in Downer Cattle with Unknown Etiology |
| title_full | Bacterial Profiles of Brain in Downer Cattle with Unknown Etiology |
| title_fullStr | Bacterial Profiles of Brain in Downer Cattle with Unknown Etiology |
| title_full_unstemmed | Bacterial Profiles of Brain in Downer Cattle with Unknown Etiology |
| title_short | Bacterial Profiles of Brain in Downer Cattle with Unknown Etiology |
| title_sort | bacterial profiles of brain in downer cattle with unknown etiology |
| topic | downer cow unknown etiology brain microbiota next-generation sequencing |
| url | https://www.mdpi.com/2076-2607/11/1/98 |
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