A Novel Acinetobacter baumannii Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative Microbes
The rapid spread and emergence of multidrug-resistant Acinetobacter baumannii and other pathogenic Gram-negative bacteria spurred scientists and clinicians to look for alternative therapeutic agents to conventional antibiotics. In the present study, an A. baumannii bacteriophage p54 was isolated and...
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| Format: | Article |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.637313/full |
| _version_ | 1818413100671434752 |
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| author | Fazal Mehmood Khan Fazal Mehmood Khan Vijay Singh Gondil Changchang Li Changchang Li Mengwei Jiang Junhua Li Junping Yu Junping Yu Hongping Wei Hongping Wei Hang Yang Hang Yang |
| author_facet | Fazal Mehmood Khan Fazal Mehmood Khan Vijay Singh Gondil Changchang Li Changchang Li Mengwei Jiang Junhua Li Junping Yu Junping Yu Hongping Wei Hongping Wei Hang Yang Hang Yang |
| author_sort | Fazal Mehmood Khan |
| collection | DOAJ |
| description | The rapid spread and emergence of multidrug-resistant Acinetobacter baumannii and other pathogenic Gram-negative bacteria spurred scientists and clinicians to look for alternative therapeutic agents to conventional antibiotics. In the present study, an A. baumannii bacteriophage p54 was isolated and characterized. Morphological and genome analysis revealed that bacteriophage p54 belongs to Myoviridae family with a genome size of 165,813 bps. A novel endolysin, namely LysAB54, showing low similarity with other well-known related endolysins, was cloned, expressed, and characterized from the bacteriophage p54. LysAB54 showed significant bactericidal activity against multidrug-resistant A. baumannii and other Gram-negative bacteria, including Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli, in the absence of outer membrane permeabilizers. Based on all those observations, LysAB54 could represent a potential agent for the treatment of multidrug-resistant Gram-negative superbugs. |
| first_indexed | 2024-12-14T10:57:50Z |
| format | Article |
| id | doaj.art-5120de0fe7664d4ea1a82ba517b3fee7 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-14T10:57:50Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-5120de0fe7664d4ea1a82ba517b3fee72022-12-21T23:04:52ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-03-011110.3389/fcimb.2021.637313637313A Novel Acinetobacter baumannii Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative MicrobesFazal Mehmood Khan0Fazal Mehmood Khan1Vijay Singh Gondil2Changchang Li3Changchang Li4Mengwei Jiang5Junhua Li6Junping Yu7Junping Yu8Hongping Wei9Hongping Wei10Hang Yang11Hang Yang12CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, ChinaInternational College, University of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, ChinaInternational College, University of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, ChinaInternational College, University of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, ChinaInternational College, University of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, ChinaInternational College, University of Chinese Academy of Sciences, Beijing, ChinaThe rapid spread and emergence of multidrug-resistant Acinetobacter baumannii and other pathogenic Gram-negative bacteria spurred scientists and clinicians to look for alternative therapeutic agents to conventional antibiotics. In the present study, an A. baumannii bacteriophage p54 was isolated and characterized. Morphological and genome analysis revealed that bacteriophage p54 belongs to Myoviridae family with a genome size of 165,813 bps. A novel endolysin, namely LysAB54, showing low similarity with other well-known related endolysins, was cloned, expressed, and characterized from the bacteriophage p54. LysAB54 showed significant bactericidal activity against multidrug-resistant A. baumannii and other Gram-negative bacteria, including Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli, in the absence of outer membrane permeabilizers. Based on all those observations, LysAB54 could represent a potential agent for the treatment of multidrug-resistant Gram-negative superbugs.https://www.frontiersin.org/articles/10.3389/fcimb.2021.637313/fullbacteriophageendolysinAcinetobacter baumanniiGram-negative “superbugs”antimicrobial resistance |
| spellingShingle | Fazal Mehmood Khan Fazal Mehmood Khan Vijay Singh Gondil Changchang Li Changchang Li Mengwei Jiang Junhua Li Junping Yu Junping Yu Hongping Wei Hongping Wei Hang Yang Hang Yang A Novel Acinetobacter baumannii Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative Microbes Frontiers in Cellular and Infection Microbiology bacteriophage endolysin Acinetobacter baumannii Gram-negative “superbugs” antimicrobial resistance |
| title | A Novel Acinetobacter baumannii Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative Microbes |
| title_full | A Novel Acinetobacter baumannii Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative Microbes |
| title_fullStr | A Novel Acinetobacter baumannii Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative Microbes |
| title_full_unstemmed | A Novel Acinetobacter baumannii Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative Microbes |
| title_short | A Novel Acinetobacter baumannii Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative Microbes |
| title_sort | novel acinetobacter baumannii bacteriophage endolysin lysab54 with high antibacterial activity against multiple gram negative microbes |
| topic | bacteriophage endolysin Acinetobacter baumannii Gram-negative “superbugs” antimicrobial resistance |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2021.637313/full |
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