Isolation of Shiga Toxin-Producing Escherichia coli from the Surfaces of Beef Carcasses in Slaughterhouses in Japan
Shiga toxin-producing E. coli (STEC) is an important foodborne pathogen worldwide. It is necessary to control and prevent STEC contamination on beef carcasses in slaughterhouses because STEC infection is associated with beef consumption. However, the frequencies of STEC contamination of beef carcass...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Journal of Food Protection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0362028X24000474 |
| _version_ | 1797246854489964544 |
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| author | Shunsuke Ikeuchi Shouhei Hirose Kohei Shimada Ayako Koyama Shoji Ishida Naoto Katayama Takehiko Suzuki Akiko Tokairin Mayumi Tsukamoto Yuki Tsue Kenichi Yamaguchi Hideo Osako Sachiko Hiwatashi Yumi Chiba Hiroshi Akiyama Hideki Hayashidani Yukiko Hara-Kudo |
| author_facet | Shunsuke Ikeuchi Shouhei Hirose Kohei Shimada Ayako Koyama Shoji Ishida Naoto Katayama Takehiko Suzuki Akiko Tokairin Mayumi Tsukamoto Yuki Tsue Kenichi Yamaguchi Hideo Osako Sachiko Hiwatashi Yumi Chiba Hiroshi Akiyama Hideki Hayashidani Yukiko Hara-Kudo |
| author_sort | Shunsuke Ikeuchi |
| collection | DOAJ |
| description | Shiga toxin-producing E. coli (STEC) is an important foodborne pathogen worldwide. It is necessary to control and prevent STEC contamination on beef carcasses in slaughterhouses because STEC infection is associated with beef consumption. However, the frequencies of STEC contamination of beef carcasses in various slaughterhouses in Japan are not well known. Herein, we investigated the contamination of beef carcasses with STEC in slaughterhouses to assess the potential risks of STEC. In total, 524 gauze samples were collected from the surfaces of beef carcasses at 12 domestic slaughterhouses from November 2020 to February 2023. The samples were measured for aerobic plate counts and tested for pathogenic genes (stx and eae) and major O-serogroups (O26, O45, O103, O111, O121, O145, and O157) by real-time PCR screening. Subsequently, immunomagnetic separation (IMS) was performed on samples positive for stx, eae, and at least one of the seven O-serogroups of STEC. Isolation process without IMS was performed on samples positive for stx, including those subjected to IMS. STEC O157:H7 and stx-positive E. coli other than serotype O157:H7 were isolated from 0.6% and 4.6% of beef carcass surfaces, respectively. Although the STEC O157:H7 isolation rate was low and stx-positive E. coli other than serotype O157:H7 belonged to minor O-serogroups, the results mean a risk of foodborne illness. Furthermore, a moderate correlation was observed between aerobic plate counts and detection rates of stx-positive samples by real-time PCR screening. The STEC O157:H7 isolated facilities showed higher values on aerobic plate counts and detection rates of stx-positive samples than the mean values of total samples. Therefore, these results suggest that it is important to evaluate hygiene treatments against beef carcasses for the reduction of STEC contamination risk, particularly in facilities with high aerobic plate counts. |
| first_indexed | 2024-04-24T19:49:25Z |
| format | Article |
| id | doaj.art-5122e79226ea4b38be0b947edc8ce1ac |
| institution | Directory Open Access Journal |
| issn | 0362-028X |
| language | English |
| last_indexed | 2024-04-24T19:49:25Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Food Protection |
| spelling | doaj.art-5122e79226ea4b38be0b947edc8ce1ac2024-03-25T04:17:08ZengElsevierJournal of Food Protection0362-028X2024-05-01875100263Isolation of Shiga Toxin-Producing Escherichia coli from the Surfaces of Beef Carcasses in Slaughterhouses in JapanShunsuke Ikeuchi0Shouhei Hirose1Kohei Shimada2Ayako Koyama3Shoji Ishida4Naoto Katayama5Takehiko Suzuki6Akiko Tokairin7Mayumi Tsukamoto8Yuki Tsue9Kenichi Yamaguchi10Hideo Osako11Sachiko Hiwatashi12Yumi Chiba13Hiroshi Akiyama14Hideki Hayashidani15Yukiko Hara-Kudo16Division of Microbiology, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan; Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, JapanDivision of Microbiology, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, JapanSection of Meat Inspection, Division of Food Hygiene, Bureau of Health and Safety, Department of Health and Welfare, Hokkaido Government, Kita 3-jo, Nishi 6-chome, Chuo-ku, Sapporo, Hokkaido 060-8588, JapanHigashimokoto Meat Inspection Center, 72-1, Chigusa, Higashimokoto, Ozora, Abashiri District, Hokkaido 099-3231, JapanHokkaido Hayakita Meat Inspection Center, 695, Toasa, Abira-cho, Yufutsu District, Hokkaido 059-1433, JapanTokushima Prefectural Meat Inspection Centre, 2-140-3 Fudohon-machi, Tokushima, Tokushima 770-0063, JapanHokkaido Obihiro Meat Inspection Center, North 2, West 25, Obihiro, Hokkaido 080-2465, JapanTowada Meat Inspection Center, 1-13, Sambongi Nozaki, Towada, Aomori 034-0001, JapanGifu Prefectural Hida Meat Inspection Office, 17-1 Maehara-machi, Takayama, Gifu 506-0048, JapanMiyazaki Prefecture Tsuno Meat Inspection Center, 15530, Kawakita, Tsuno-cho, Koyu-gun, Miyazaki 889-1201, JapanAkita City Meat Hygiene Inspection Office, 2-6 Dosaka, Jinnai, Kawabe, Akita, Akita 019-2631, JapanKumamoto Prefectural Meat Inspection Office, 1341 Sozaki, Shichijyo-machi, Kikuchi, Kumamoto 861-1344, JapanNagasaki Prefectural Isahaya Meat Inspection Station, 79-20 Saiwai-machi, Isahaya, Nagasaki 854-0022, JapanDivision of Microbiology, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, JapanDepartment of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, JapanCooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan; Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, JapanDivision of Microbiology, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan; Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan; Corresponding author.Shiga toxin-producing E. coli (STEC) is an important foodborne pathogen worldwide. It is necessary to control and prevent STEC contamination on beef carcasses in slaughterhouses because STEC infection is associated with beef consumption. However, the frequencies of STEC contamination of beef carcasses in various slaughterhouses in Japan are not well known. Herein, we investigated the contamination of beef carcasses with STEC in slaughterhouses to assess the potential risks of STEC. In total, 524 gauze samples were collected from the surfaces of beef carcasses at 12 domestic slaughterhouses from November 2020 to February 2023. The samples were measured for aerobic plate counts and tested for pathogenic genes (stx and eae) and major O-serogroups (O26, O45, O103, O111, O121, O145, and O157) by real-time PCR screening. Subsequently, immunomagnetic separation (IMS) was performed on samples positive for stx, eae, and at least one of the seven O-serogroups of STEC. Isolation process without IMS was performed on samples positive for stx, including those subjected to IMS. STEC O157:H7 and stx-positive E. coli other than serotype O157:H7 were isolated from 0.6% and 4.6% of beef carcass surfaces, respectively. Although the STEC O157:H7 isolation rate was low and stx-positive E. coli other than serotype O157:H7 belonged to minor O-serogroups, the results mean a risk of foodborne illness. Furthermore, a moderate correlation was observed between aerobic plate counts and detection rates of stx-positive samples by real-time PCR screening. The STEC O157:H7 isolated facilities showed higher values on aerobic plate counts and detection rates of stx-positive samples than the mean values of total samples. Therefore, these results suggest that it is important to evaluate hygiene treatments against beef carcasses for the reduction of STEC contamination risk, particularly in facilities with high aerobic plate counts.http://www.sciencedirect.com/science/article/pii/S0362028X24000474Aerobic plate countBeef carcassContaminationIsolationSlaughterhouseSTEC |
| spellingShingle | Shunsuke Ikeuchi Shouhei Hirose Kohei Shimada Ayako Koyama Shoji Ishida Naoto Katayama Takehiko Suzuki Akiko Tokairin Mayumi Tsukamoto Yuki Tsue Kenichi Yamaguchi Hideo Osako Sachiko Hiwatashi Yumi Chiba Hiroshi Akiyama Hideki Hayashidani Yukiko Hara-Kudo Isolation of Shiga Toxin-Producing Escherichia coli from the Surfaces of Beef Carcasses in Slaughterhouses in Japan Journal of Food Protection Aerobic plate count Beef carcass Contamination Isolation Slaughterhouse STEC |
| title | Isolation of Shiga Toxin-Producing Escherichia coli from the Surfaces of Beef Carcasses in Slaughterhouses in Japan |
| title_full | Isolation of Shiga Toxin-Producing Escherichia coli from the Surfaces of Beef Carcasses in Slaughterhouses in Japan |
| title_fullStr | Isolation of Shiga Toxin-Producing Escherichia coli from the Surfaces of Beef Carcasses in Slaughterhouses in Japan |
| title_full_unstemmed | Isolation of Shiga Toxin-Producing Escherichia coli from the Surfaces of Beef Carcasses in Slaughterhouses in Japan |
| title_short | Isolation of Shiga Toxin-Producing Escherichia coli from the Surfaces of Beef Carcasses in Slaughterhouses in Japan |
| title_sort | isolation of shiga toxin producing escherichia coli from the surfaces of beef carcasses in slaughterhouses in japan |
| topic | Aerobic plate count Beef carcass Contamination Isolation Slaughterhouse STEC |
| url | http://www.sciencedirect.com/science/article/pii/S0362028X24000474 |
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