Endogenous salicylic acid suppresses de novo root regeneration from leaf explants.
Plants can regenerate new organs from damaged or detached tissues. In the process of de novo root regeneration (DNRR), adventitious roots are frequently formed from the wound site on a detached leaf. Salicylic acid (SA) is a key phytohormone regulating plant defenses and stress responses. The role o...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-03-01
|
| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1010636 |
| _version_ | 1797850294942433280 |
|---|---|
| author | Sorrel Tran Madalene Ison Nathália Cássia Ferreira Dias Maria Andrea Ortega Yun-Fan Stephanie Chen Alan Peper Lanxi Hu Dawei Xu Khadijeh Mozaffari Paul M Severns Yao Yao Chung-Jui Tsai Paulo José Pereira Lima Teixeira Li Yang |
| author_facet | Sorrel Tran Madalene Ison Nathália Cássia Ferreira Dias Maria Andrea Ortega Yun-Fan Stephanie Chen Alan Peper Lanxi Hu Dawei Xu Khadijeh Mozaffari Paul M Severns Yao Yao Chung-Jui Tsai Paulo José Pereira Lima Teixeira Li Yang |
| author_sort | Sorrel Tran |
| collection | DOAJ |
| description | Plants can regenerate new organs from damaged or detached tissues. In the process of de novo root regeneration (DNRR), adventitious roots are frequently formed from the wound site on a detached leaf. Salicylic acid (SA) is a key phytohormone regulating plant defenses and stress responses. The role of SA and its acting mechanisms during de novo organogenesis is still unclear. Here, we found that endogenous SA inhibited the adventitious root formation after cutting. Free SA rapidly accumulated at the wound site, which was accompanied by an activation of SA response. SA receptors NPR3 and NPR4, but not NPR1, were required for DNRR. Wounding-elevated SA compromised the expression of AUX1, and subsequent transport of auxin to the wound site. A mutation in AUX1 abolished the enhanced DNRR in low SA mutants. Our work elucidates a role of SA in regulating DNRR and suggests a potential link between biotic stress and tissue regeneration. |
| first_indexed | 2024-04-09T18:59:10Z |
| format | Article |
| id | doaj.art-512347d0bf17445586d6924b5350841f |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-09T18:59:10Z |
| publishDate | 2023-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-512347d0bf17445586d6924b5350841f2023-04-09T05:31:58ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042023-03-01193e101063610.1371/journal.pgen.1010636Endogenous salicylic acid suppresses de novo root regeneration from leaf explants.Sorrel TranMadalene IsonNathália Cássia Ferreira DiasMaria Andrea OrtegaYun-Fan Stephanie ChenAlan PeperLanxi HuDawei XuKhadijeh MozaffariPaul M SevernsYao YaoChung-Jui TsaiPaulo José Pereira Lima TeixeiraLi YangPlants can regenerate new organs from damaged or detached tissues. In the process of de novo root regeneration (DNRR), adventitious roots are frequently formed from the wound site on a detached leaf. Salicylic acid (SA) is a key phytohormone regulating plant defenses and stress responses. The role of SA and its acting mechanisms during de novo organogenesis is still unclear. Here, we found that endogenous SA inhibited the adventitious root formation after cutting. Free SA rapidly accumulated at the wound site, which was accompanied by an activation of SA response. SA receptors NPR3 and NPR4, but not NPR1, were required for DNRR. Wounding-elevated SA compromised the expression of AUX1, and subsequent transport of auxin to the wound site. A mutation in AUX1 abolished the enhanced DNRR in low SA mutants. Our work elucidates a role of SA in regulating DNRR and suggests a potential link between biotic stress and tissue regeneration.https://doi.org/10.1371/journal.pgen.1010636 |
| spellingShingle | Sorrel Tran Madalene Ison Nathália Cássia Ferreira Dias Maria Andrea Ortega Yun-Fan Stephanie Chen Alan Peper Lanxi Hu Dawei Xu Khadijeh Mozaffari Paul M Severns Yao Yao Chung-Jui Tsai Paulo José Pereira Lima Teixeira Li Yang Endogenous salicylic acid suppresses de novo root regeneration from leaf explants. PLoS Genetics |
| title | Endogenous salicylic acid suppresses de novo root regeneration from leaf explants. |
| title_full | Endogenous salicylic acid suppresses de novo root regeneration from leaf explants. |
| title_fullStr | Endogenous salicylic acid suppresses de novo root regeneration from leaf explants. |
| title_full_unstemmed | Endogenous salicylic acid suppresses de novo root regeneration from leaf explants. |
| title_short | Endogenous salicylic acid suppresses de novo root regeneration from leaf explants. |
| title_sort | endogenous salicylic acid suppresses de novo root regeneration from leaf explants |
| url | https://doi.org/10.1371/journal.pgen.1010636 |
| work_keys_str_mv | AT sorreltran endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants AT madaleneison endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants AT nathaliacassiaferreiradias endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants AT mariaandreaortega endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants AT yunfanstephaniechen endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants AT alanpeper endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants AT lanxihu endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants AT daweixu endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants AT khadijehmozaffari endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants AT paulmseverns endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants AT yaoyao endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants AT chungjuitsai endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants AT paulojosepereiralimateixeira endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants AT liyang endogenoussalicylicacidsuppressesdenovorootregenerationfromleafexplants |