Isolation of endothelial cells, pericytes and astrocytes from mouse brain.

Primary cell isolation from the central nervous system (CNS) has allowed fundamental understanding of blood-brain barrier (BBB) properties. However, poorly described isolation techniques or suboptimal cellular purity has been a weak point of some published scientific articles. Here, we describe in d...

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Bibliographic Details
Main Authors: Florian Bernard-Patrzynski, Marc-André Lécuyer, Ina Puscas, Imane Boukhatem, Marc Charabati, Lyne Bourbonnière, Charles Ramassamy, Grégoire Leclair, Alexandre Prat, V Gaëlle Roullin
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0226302