Half-life of Glycated Tryptophan in the Plasma of Chickens
Tryptophan, an essential amino acid, is enzymatically metabolized to two compounds, kynurenine and serotonin, and 95% of tryptophan is metabolized to kynurenine. As chickens have hyperglycemia and high temperature, tryptophan glycation occurs more easily in chickens than in mammals. Part of tryptoph...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2018-04-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/55/2/55_0170158/_html/-char/en |
| _version_ | 1797817006341226496 |
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| author | Ryosuke Makino Kazumi Kita |
| author_facet | Ryosuke Makino Kazumi Kita |
| author_sort | Ryosuke Makino |
| collection | DOAJ |
| description | Tryptophan, an essential amino acid, is enzymatically metabolized to two compounds, kynurenine and serotonin, and 95% of tryptophan is metabolized to kynurenine. As chickens have hyperglycemia and high temperature, tryptophan glycation occurs more easily in chickens than in mammals. Part of tryptophan is non-enzymatically converted to two types of glycated tryptophan, tryptophan-Amadori product and (1R, 3S)-1-(d-gluco-1, 2, 3, 4, 5-pentahydroxypentyl)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (PHP-THβC). Although these compounds are detected in the plasma of chickens, information on the half-life of PHP-THβC in the blood circulation is limited. Therefore, the present study aimed to measure the half-life of plasma PHP-THβC in chickens. PHP-THβC (114 nmol/0.2 mL/70 g body weight) was intravenously administered to chickens via the wing vein, and blood samples were collected at 0, 15, 30, 60, 180, 360, 720, and 1440 min after administration. Plasma concentrations of PHP-THβC were measured by liquid chromatography-mass spectrometry. Plasma PHP-THβC reached to a peak concentration of 16.1 βM at 30 min after administration, and then decreased rapidly to return to the physiological level (0 min) at 360 min after administration. The half-life of plasma PHP-THβC was calculated by non-linear regression analysis, and it was found to be 107 min. This study was the first to measure plasma half-life of glycated tryptophan. |
| first_indexed | 2024-03-13T08:46:01Z |
| format | Article |
| id | doaj.art-5125a856c3df4355b876eb3b640507d5 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:46:01Z |
| publishDate | 2018-04-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-5125a856c3df4355b876eb3b640507d52023-05-30T02:01:16ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862018-04-0155211711910.2141/jpsa.0170158jpsaHalf-life of Glycated Tryptophan in the Plasma of ChickensRyosuke Makino0Kazumi Kita1Laboratory of Animal Production, Department of Agrobiological Science, Faculty of Agriculture, Ehime UniversityLaboratory of Animal Nutrition, Faculty of Agriculture, Iwate UniversityTryptophan, an essential amino acid, is enzymatically metabolized to two compounds, kynurenine and serotonin, and 95% of tryptophan is metabolized to kynurenine. As chickens have hyperglycemia and high temperature, tryptophan glycation occurs more easily in chickens than in mammals. Part of tryptophan is non-enzymatically converted to two types of glycated tryptophan, tryptophan-Amadori product and (1R, 3S)-1-(d-gluco-1, 2, 3, 4, 5-pentahydroxypentyl)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (PHP-THβC). Although these compounds are detected in the plasma of chickens, information on the half-life of PHP-THβC in the blood circulation is limited. Therefore, the present study aimed to measure the half-life of plasma PHP-THβC in chickens. PHP-THβC (114 nmol/0.2 mL/70 g body weight) was intravenously administered to chickens via the wing vein, and blood samples were collected at 0, 15, 30, 60, 180, 360, 720, and 1440 min after administration. Plasma concentrations of PHP-THβC were measured by liquid chromatography-mass spectrometry. Plasma PHP-THβC reached to a peak concentration of 16.1 βM at 30 min after administration, and then decreased rapidly to return to the physiological level (0 min) at 360 min after administration. The half-life of plasma PHP-THβC was calculated by non-linear regression analysis, and it was found to be 107 min. This study was the first to measure plasma half-life of glycated tryptophan.https://www.jstage.jst.go.jp/article/jpsa/55/2/55_0170158/_html/-char/enchickenglycationphp-thβcplasma half-lifetryptophan |
| spellingShingle | Ryosuke Makino Kazumi Kita Half-life of Glycated Tryptophan in the Plasma of Chickens The Journal of Poultry Science chicken glycation php-thβc plasma half-life tryptophan |
| title | Half-life of Glycated Tryptophan in the Plasma of Chickens |
| title_full | Half-life of Glycated Tryptophan in the Plasma of Chickens |
| title_fullStr | Half-life of Glycated Tryptophan in the Plasma of Chickens |
| title_full_unstemmed | Half-life of Glycated Tryptophan in the Plasma of Chickens |
| title_short | Half-life of Glycated Tryptophan in the Plasma of Chickens |
| title_sort | half life of glycated tryptophan in the plasma of chickens |
| topic | chicken glycation php-thβc plasma half-life tryptophan |
| url | https://www.jstage.jst.go.jp/article/jpsa/55/2/55_0170158/_html/-char/en |
| work_keys_str_mv | AT ryosukemakino halflifeofglycatedtryptophanintheplasmaofchickens AT kazumikita halflifeofglycatedtryptophanintheplasmaofchickens |