Evaluation of Light-Emitting Diodes’ Effects on the Expression Level of P53 and EGFR in the Gingival Tissues of Albino Rats
<i>Background and objectives</i>: The light-curing unit is considered an essential piece of equipment in every dental office. This study was conducted to evaluate the effect of Light-Emitting Diodes (LEDs) by the light cure (LC) device on gingival tissues of albino rats histologically an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1010-660X/55/9/605 |
| _version_ | 1797710229296644096 |
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| author | Azhar Ghanim Ahmed Alaa Hani Raziq |
| author_facet | Azhar Ghanim Ahmed Alaa Hani Raziq |
| author_sort | Azhar Ghanim Ahmed |
| collection | DOAJ |
| description | <i>Background and objectives</i>: The light-curing unit is considered an essential piece of equipment in every dental office. This study was conducted to evaluate the effect of Light-Emitting Diodes (LEDs) by the light cure (LC) device on gingival tissues of albino rats histologically and by regarding the expression of P53 and epidermal growth factor receptor (EGFR). <i>Materials and methods</i>: Gingival tissues of the rats were exposed to LEDs for 30 s with an interval of 30 s for periods of 2 and 5 min and were examined after two and four weeks of light exposure. After the set time, histological sections were studied and the P53 and EGFR expressions were evaluated immunohistochemically and by molecular methods. <i>Results</i>: Mild hyperplasia and mild inflammatory response were detected in higher rates after two weeks of exposure when compared to 4 weeks postexposure. Whereas fibrosis was found at a higher rate after four weeks than that found after two weeks postexposure, parakeratosis was seen only in the group that was exposed for 5 min to LC and when biopsies were taken after 2 weeks. We found that the immunohistochemical expression of P53 was not changed. Similarly, the alteration of EGFR expression was statistically nonsignificant (<i>p</i> > 0.05) when compared to the control group. The data obtained from the qRT-PCR reaction was analyzed using the comparative CT (2<sup>−ΔΔCT</sup>) method. Statistically, there was no significant difference in the expression of EGER and P53 gene transcripts. <i>Conclusions</i>: LED causes no serious alteration in P53 and EGFR expression, and only trivial histopathological changes occurred, most of which recovered after a 4-week interval. |
| first_indexed | 2024-03-12T06:48:10Z |
| format | Article |
| id | doaj.art-51264aae0160485e857f20f42c8bf283 |
| institution | Directory Open Access Journal |
| issn | 1010-660X |
| language | English |
| last_indexed | 2024-03-12T06:48:10Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-51264aae0160485e857f20f42c8bf2832023-09-03T00:26:24ZengMDPI AGMedicina1010-660X2019-09-0155960510.3390/medicina55090605medicina55090605Evaluation of Light-Emitting Diodes’ Effects on the Expression Level of P53 and EGFR in the Gingival Tissues of Albino RatsAzhar Ghanim Ahmed0Alaa Hani Raziq1Basic Sciences Department College of Dentistry, University of Sulaimani, Sulaimani 46001, IraqPathology Department, College of Medicine, Duhok University, Duhok 42001, Iraq<i>Background and objectives</i>: The light-curing unit is considered an essential piece of equipment in every dental office. This study was conducted to evaluate the effect of Light-Emitting Diodes (LEDs) by the light cure (LC) device on gingival tissues of albino rats histologically and by regarding the expression of P53 and epidermal growth factor receptor (EGFR). <i>Materials and methods</i>: Gingival tissues of the rats were exposed to LEDs for 30 s with an interval of 30 s for periods of 2 and 5 min and were examined after two and four weeks of light exposure. After the set time, histological sections were studied and the P53 and EGFR expressions were evaluated immunohistochemically and by molecular methods. <i>Results</i>: Mild hyperplasia and mild inflammatory response were detected in higher rates after two weeks of exposure when compared to 4 weeks postexposure. Whereas fibrosis was found at a higher rate after four weeks than that found after two weeks postexposure, parakeratosis was seen only in the group that was exposed for 5 min to LC and when biopsies were taken after 2 weeks. We found that the immunohistochemical expression of P53 was not changed. Similarly, the alteration of EGFR expression was statistically nonsignificant (<i>p</i> > 0.05) when compared to the control group. The data obtained from the qRT-PCR reaction was analyzed using the comparative CT (2<sup>−ΔΔCT</sup>) method. Statistically, there was no significant difference in the expression of EGER and P53 gene transcripts. <i>Conclusions</i>: LED causes no serious alteration in P53 and EGFR expression, and only trivial histopathological changes occurred, most of which recovered after a 4-week interval.https://www.mdpi.com/1010-660X/55/9/605dental curing lightsblue light hazardP53EGFRqRT-PCR |
| spellingShingle | Azhar Ghanim Ahmed Alaa Hani Raziq Evaluation of Light-Emitting Diodes’ Effects on the Expression Level of P53 and EGFR in the Gingival Tissues of Albino Rats Medicina dental curing lights blue light hazard P53 EGFR qRT-PCR |
| title | Evaluation of Light-Emitting Diodes’ Effects on the Expression Level of P53 and EGFR in the Gingival Tissues of Albino Rats |
| title_full | Evaluation of Light-Emitting Diodes’ Effects on the Expression Level of P53 and EGFR in the Gingival Tissues of Albino Rats |
| title_fullStr | Evaluation of Light-Emitting Diodes’ Effects on the Expression Level of P53 and EGFR in the Gingival Tissues of Albino Rats |
| title_full_unstemmed | Evaluation of Light-Emitting Diodes’ Effects on the Expression Level of P53 and EGFR in the Gingival Tissues of Albino Rats |
| title_short | Evaluation of Light-Emitting Diodes’ Effects on the Expression Level of P53 and EGFR in the Gingival Tissues of Albino Rats |
| title_sort | evaluation of light emitting diodes effects on the expression level of p53 and egfr in the gingival tissues of albino rats |
| topic | dental curing lights blue light hazard P53 EGFR qRT-PCR |
| url | https://www.mdpi.com/1010-660X/55/9/605 |
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