NIR-Emitting Alloyed CdTeSe QDs and Organic Dye Assemblies: A Nontoxic, Stable, and Efficient FRET System
In the present work, we synthesize Near Infrared (NIR)-emitting alloyed mercaptopropionic acid (MPA)-capped CdTeSe quantum dots (QDs) in a single-step one-hour process, without the use of an inert atmosphere or any pyrophoric ligands. The quantum dots are water soluble, non-toxic, and highly photost...
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| Format: | Article |
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MDPI AG
2018-04-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/8/4/231 |
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| author | Doris E. Ramírez-Herrera Eustolia Rodríguez-Velázquez Manuel Alatorre-Meda Francisco Paraguay-Delgado Antonio Tirado-Guízar Pablo Taboada Georgina Pina-Luis |
| author_facet | Doris E. Ramírez-Herrera Eustolia Rodríguez-Velázquez Manuel Alatorre-Meda Francisco Paraguay-Delgado Antonio Tirado-Guízar Pablo Taboada Georgina Pina-Luis |
| author_sort | Doris E. Ramírez-Herrera |
| collection | DOAJ |
| description | In the present work, we synthesize Near Infrared (NIR)-emitting alloyed mercaptopropionic acid (MPA)-capped CdTeSe quantum dots (QDs) in a single-step one-hour process, without the use of an inert atmosphere or any pyrophoric ligands. The quantum dots are water soluble, non-toxic, and highly photostable and have high quantum yields (QYs) up to 84%. The alloyed MPA-capped CdTeSe QDs exhibit a red-shifted emission, whose color can be tuned between visible and NIR regions (608–750 nm) by controlling the Te:Se molar ratio in the precursor mixtures and/or changing the time reaction. The MPA-capped QDs were characterized by UV-visible absorption spectroscopy, fluorescence spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), and zeta potential measurements. Photostability studies were performed by irradiating the QDs with a high-power xenon lamp. The ternary MPA-CdTeSe QDs showed greater photostability than the corresponding binary MPA-CdTe QDs. We report the Förster resonance energy transfer (FRET) from the MPA-capped CdTeSe QDs as energy donors and Cyanine5 NHS-ester (Cy5) dye as an energy acceptor with efficiency (E) up to 95%. The distance between the QDs and dye (r), the Förster distance (R0), and the binding constant (K) are reported. Additionally, cytocompatibility and cell internalization experiments conducted on human cancer cells (HeLa) cells revealed that alloyed MPA-capped CdTeSe QDs are more cytocompatible than MPA-capped CdTe QDs and are capable of ordering homogeneously all over the cytoplasm, which allows their use as potential safe, green donors for biological FRET applications. |
| first_indexed | 2024-12-21T18:33:30Z |
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| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-21T18:33:30Z |
| publishDate | 2018-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-512731ecfa7a422f8c89c545531ee7f52022-12-21T18:54:13ZengMDPI AGNanomaterials2079-49912018-04-018423110.3390/nano8040231nano8040231NIR-Emitting Alloyed CdTeSe QDs and Organic Dye Assemblies: A Nontoxic, Stable, and Efficient FRET SystemDoris E. Ramírez-Herrera0Eustolia Rodríguez-Velázquez1Manuel Alatorre-Meda2Francisco Paraguay-Delgado3Antonio Tirado-Guízar4Pablo Taboada5Georgina Pina-Luis6Centro de Graduados e Investigación, Instituto Tecnológico de Tijuana, A.P. 1166, 22500 Tijuana, BC, MexicoFacultad de Odontología, Universidad Autónoma de Baja California, Calzada Universidad 14418, Parque Industrial Internacional, 22390 Tijuana, BC, MexicoInstituto de Ortopedia y Banco de Tejidos Musculoesqueléticos, Universidad de Santiago de Compostela, Campus Sur S/N, E-15782 Santiago de Compostela, SpainCentro de Investigación en Materiales Avanzados S. C., Departamento de Física de Materiales, Av. Miguel de Cervantes 120, Complejo Industrial Chihuahua, CP 31109 Chihuahua, Chih., MexicoCentro de Graduados e Investigación, Instituto Tecnológico de Tijuana, A.P. 1166, 22500 Tijuana, BC, MexicoGrupo de Física de Coloides y Polímeros, Departamento de Física de Materia Condensada, Facultad de Física, Universidad de Santiago de Compostela, Campus Sur S/N, E-15782 Santiago de Compostela, SpainCentro de Graduados e Investigación, Instituto Tecnológico de Tijuana, A.P. 1166, 22500 Tijuana, BC, MexicoIn the present work, we synthesize Near Infrared (NIR)-emitting alloyed mercaptopropionic acid (MPA)-capped CdTeSe quantum dots (QDs) in a single-step one-hour process, without the use of an inert atmosphere or any pyrophoric ligands. The quantum dots are water soluble, non-toxic, and highly photostable and have high quantum yields (QYs) up to 84%. The alloyed MPA-capped CdTeSe QDs exhibit a red-shifted emission, whose color can be tuned between visible and NIR regions (608–750 nm) by controlling the Te:Se molar ratio in the precursor mixtures and/or changing the time reaction. The MPA-capped QDs were characterized by UV-visible absorption spectroscopy, fluorescence spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), and zeta potential measurements. Photostability studies were performed by irradiating the QDs with a high-power xenon lamp. The ternary MPA-CdTeSe QDs showed greater photostability than the corresponding binary MPA-CdTe QDs. We report the Förster resonance energy transfer (FRET) from the MPA-capped CdTeSe QDs as energy donors and Cyanine5 NHS-ester (Cy5) dye as an energy acceptor with efficiency (E) up to 95%. The distance between the QDs and dye (r), the Förster distance (R0), and the binding constant (K) are reported. Additionally, cytocompatibility and cell internalization experiments conducted on human cancer cells (HeLa) cells revealed that alloyed MPA-capped CdTeSe QDs are more cytocompatible than MPA-capped CdTe QDs and are capable of ordering homogeneously all over the cytoplasm, which allows their use as potential safe, green donors for biological FRET applications.http://www.mdpi.com/2079-4991/8/4/231energy transferalloyed quantum dotsquantum dots-dye assembliescytocompatibility |
| spellingShingle | Doris E. Ramírez-Herrera Eustolia Rodríguez-Velázquez Manuel Alatorre-Meda Francisco Paraguay-Delgado Antonio Tirado-Guízar Pablo Taboada Georgina Pina-Luis NIR-Emitting Alloyed CdTeSe QDs and Organic Dye Assemblies: A Nontoxic, Stable, and Efficient FRET System Nanomaterials energy transfer alloyed quantum dots quantum dots-dye assemblies cytocompatibility |
| title | NIR-Emitting Alloyed CdTeSe QDs and Organic Dye Assemblies: A Nontoxic, Stable, and Efficient FRET System |
| title_full | NIR-Emitting Alloyed CdTeSe QDs and Organic Dye Assemblies: A Nontoxic, Stable, and Efficient FRET System |
| title_fullStr | NIR-Emitting Alloyed CdTeSe QDs and Organic Dye Assemblies: A Nontoxic, Stable, and Efficient FRET System |
| title_full_unstemmed | NIR-Emitting Alloyed CdTeSe QDs and Organic Dye Assemblies: A Nontoxic, Stable, and Efficient FRET System |
| title_short | NIR-Emitting Alloyed CdTeSe QDs and Organic Dye Assemblies: A Nontoxic, Stable, and Efficient FRET System |
| title_sort | nir emitting alloyed cdtese qds and organic dye assemblies a nontoxic stable and efficient fret system |
| topic | energy transfer alloyed quantum dots quantum dots-dye assemblies cytocompatibility |
| url | http://www.mdpi.com/2079-4991/8/4/231 |
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