Anti-HIV-1 activity of a new scorpion venom peptide derivative Kn2-7.
For over 30 years, HIV/AIDS has wreaked havoc in the world. In the absence of an effective vaccine for HIV, development of new anti-HIV agents is urgently needed. We previously identified the antiviral activities of the scorpion-venom-peptide-derived mucroporin-M1 for three RNA viruses (measles viru...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3334916?pdf=render |
| _version_ | 1818042627068526592 |
|---|---|
| author | Yaoqing Chen Luyang Cao Maohua Zhong Yan Zhang Chen Han Qiaoli Li Jingyi Yang Dihan Zhou Wei Shi Benxia He Fang Liu Jie Yu Ying Sun Yuan Cao Yaoming Li Wenxin Li Deying Guo Zhijian Cao Huimin Yan |
| author_facet | Yaoqing Chen Luyang Cao Maohua Zhong Yan Zhang Chen Han Qiaoli Li Jingyi Yang Dihan Zhou Wei Shi Benxia He Fang Liu Jie Yu Ying Sun Yuan Cao Yaoming Li Wenxin Li Deying Guo Zhijian Cao Huimin Yan |
| author_sort | Yaoqing Chen |
| collection | DOAJ |
| description | For over 30 years, HIV/AIDS has wreaked havoc in the world. In the absence of an effective vaccine for HIV, development of new anti-HIV agents is urgently needed. We previously identified the antiviral activities of the scorpion-venom-peptide-derived mucroporin-M1 for three RNA viruses (measles viruses, SARS-CoV, and H5N1). In this investigation, a panel of scorpion venom peptides and their derivatives were designed and chosen for assessment of their anti-HIV activities. A new scorpion venom peptide derivative Kn2-7 was identified as the most potent anti-HIV-1 peptide by screening assays with an EC(50) value of 2.76 µg/ml (1.65 µM) and showed low cytotoxicity to host cells with a selective index (SI) of 13.93. Kn2-7 could inhibit all members of a standard reference panel of HIV-1 subtype B pseudotyped virus (PV) with CCR5-tropic and CXCR4-tropic NL4-3 PV strain. Furthermore, it also inhibited a CXCR4-tropic replication-competent strain of HIV-1 subtype B virus. Binding assay of Kn2-7 to HIV-1 PV by Octet Red system suggested the anti-HIV-1 activity was correlated with a direct interaction between Kn2-7 and HIV-1 envelope. These results demonstrated that peptide Kn2-7 could inhibit HIV-1 by direct interaction with viral particle and may become a promising candidate compound for further development of microbicide against HIV-1. |
| first_indexed | 2024-12-10T08:49:19Z |
| format | Article |
| id | doaj.art-512a5111e47e45cab10a46a5c9612cd3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T08:49:19Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-512a5111e47e45cab10a46a5c9612cd32022-12-22T01:55:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3494710.1371/journal.pone.0034947Anti-HIV-1 activity of a new scorpion venom peptide derivative Kn2-7.Yaoqing ChenLuyang CaoMaohua ZhongYan ZhangChen HanQiaoli LiJingyi YangDihan ZhouWei ShiBenxia HeFang LiuJie YuYing SunYuan CaoYaoming LiWenxin LiDeying GuoZhijian CaoHuimin YanFor over 30 years, HIV/AIDS has wreaked havoc in the world. In the absence of an effective vaccine for HIV, development of new anti-HIV agents is urgently needed. We previously identified the antiviral activities of the scorpion-venom-peptide-derived mucroporin-M1 for three RNA viruses (measles viruses, SARS-CoV, and H5N1). In this investigation, a panel of scorpion venom peptides and their derivatives were designed and chosen for assessment of their anti-HIV activities. A new scorpion venom peptide derivative Kn2-7 was identified as the most potent anti-HIV-1 peptide by screening assays with an EC(50) value of 2.76 µg/ml (1.65 µM) and showed low cytotoxicity to host cells with a selective index (SI) of 13.93. Kn2-7 could inhibit all members of a standard reference panel of HIV-1 subtype B pseudotyped virus (PV) with CCR5-tropic and CXCR4-tropic NL4-3 PV strain. Furthermore, it also inhibited a CXCR4-tropic replication-competent strain of HIV-1 subtype B virus. Binding assay of Kn2-7 to HIV-1 PV by Octet Red system suggested the anti-HIV-1 activity was correlated with a direct interaction between Kn2-7 and HIV-1 envelope. These results demonstrated that peptide Kn2-7 could inhibit HIV-1 by direct interaction with viral particle and may become a promising candidate compound for further development of microbicide against HIV-1.http://europepmc.org/articles/PMC3334916?pdf=render |
| spellingShingle | Yaoqing Chen Luyang Cao Maohua Zhong Yan Zhang Chen Han Qiaoli Li Jingyi Yang Dihan Zhou Wei Shi Benxia He Fang Liu Jie Yu Ying Sun Yuan Cao Yaoming Li Wenxin Li Deying Guo Zhijian Cao Huimin Yan Anti-HIV-1 activity of a new scorpion venom peptide derivative Kn2-7. PLoS ONE |
| title | Anti-HIV-1 activity of a new scorpion venom peptide derivative Kn2-7. |
| title_full | Anti-HIV-1 activity of a new scorpion venom peptide derivative Kn2-7. |
| title_fullStr | Anti-HIV-1 activity of a new scorpion venom peptide derivative Kn2-7. |
| title_full_unstemmed | Anti-HIV-1 activity of a new scorpion venom peptide derivative Kn2-7. |
| title_short | Anti-HIV-1 activity of a new scorpion venom peptide derivative Kn2-7. |
| title_sort | anti hiv 1 activity of a new scorpion venom peptide derivative kn2 7 |
| url | http://europepmc.org/articles/PMC3334916?pdf=render |
| work_keys_str_mv | AT yaoqingchen antihiv1activityofanewscorpionvenompeptidederivativekn27 AT luyangcao antihiv1activityofanewscorpionvenompeptidederivativekn27 AT maohuazhong antihiv1activityofanewscorpionvenompeptidederivativekn27 AT yanzhang antihiv1activityofanewscorpionvenompeptidederivativekn27 AT chenhan antihiv1activityofanewscorpionvenompeptidederivativekn27 AT qiaolili antihiv1activityofanewscorpionvenompeptidederivativekn27 AT jingyiyang antihiv1activityofanewscorpionvenompeptidederivativekn27 AT dihanzhou antihiv1activityofanewscorpionvenompeptidederivativekn27 AT weishi antihiv1activityofanewscorpionvenompeptidederivativekn27 AT benxiahe antihiv1activityofanewscorpionvenompeptidederivativekn27 AT fangliu antihiv1activityofanewscorpionvenompeptidederivativekn27 AT jieyu antihiv1activityofanewscorpionvenompeptidederivativekn27 AT yingsun antihiv1activityofanewscorpionvenompeptidederivativekn27 AT yuancao antihiv1activityofanewscorpionvenompeptidederivativekn27 AT yaomingli antihiv1activityofanewscorpionvenompeptidederivativekn27 AT wenxinli antihiv1activityofanewscorpionvenompeptidederivativekn27 AT deyingguo antihiv1activityofanewscorpionvenompeptidederivativekn27 AT zhijiancao antihiv1activityofanewscorpionvenompeptidederivativekn27 AT huiminyan antihiv1activityofanewscorpionvenompeptidederivativekn27 |