Assessing the feasibility of exogenous 17β-estradiol for inducing sex change in white snook, C. viridis: From growth, resting and maturation studies
This study evaluated growth, survival, and oogenesis before and after 17β-estradiol (E2) treatments. During the growth period, C. viridis reached a total length of 65 ± 4.2 cm and a weight of 2.5 ± 0.66 kg BW. To induce sex change in males, we implanted silastic tubing loaded with 0 (control), 0.5,...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342300306X |
| _version_ | 1827582064458727424 |
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| author | Navarro-Flores Jaime Martínez-Brown Juan Manuel Zavala-Leal Iram Rojo-Cebreros Angel Humberto Ibarra-Castro Leonardo |
| author_facet | Navarro-Flores Jaime Martínez-Brown Juan Manuel Zavala-Leal Iram Rojo-Cebreros Angel Humberto Ibarra-Castro Leonardo |
| author_sort | Navarro-Flores Jaime |
| collection | DOAJ |
| description | This study evaluated growth, survival, and oogenesis before and after 17β-estradiol (E2) treatments. During the growth period, C. viridis reached a total length of 65 ± 4.2 cm and a weight of 2.5 ± 0.66 kg BW. To induce sex change in males, we implanted silastic tubing loaded with 0 (control), 0.5, or 1.5 mg of E2 kg−1, at days 0, 30, 60, and 90. Blood samples were collected before treatment (day 0) and on days 30, 60, 90, and 120. Initially, 23% of the selected males expressed milt. At 60 days post-treatment 13% in the control and 4.3% in both, 0.5 and 1.5 mg E2 kg−1 BW treatments. After 120 days we observed histological evidence of sex change in 50% of the histology-tagged fish in the 1.5 mg E2 kg−1 treatment. The gonadosomatic index and condition factor did not differ significantly (P > 0.05) between the control and the treatments. However, the mean individual fish weight differed significantly across all sampling times (P < 0.05). The control and 1.5 treatment groups had a 100% survival rate, while the 0.5 treatment showed a 95.6% survival rate. Steroid profiling showed high cortisol levels throughout the experiment but there was no relationship with E2 treatment or sampling point. After the first E2 dose, we observed inhibition of 11-ketotestosterone (11-KT), which persisted until the end of the experiment. At the end of a 150-day rest period, biopsy samples from the fish showed 0%, 29%, and 87% of females with primary growth oocytes in the control, 0.5, and 1.5 treatments, respectively. After 353 days of the maturation period, feminized fish did not reach vitellogenesis. The availability of C. viridis vitellogenic females after induced sex change remains unpredictable. More research is needed to encourage vitellogenesis in hormonally feminized fish of this species. |
| first_indexed | 2024-03-08T22:46:09Z |
| format | Article |
| id | doaj.art-512c39663791457d966f0a7b6a3f3fc0 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-08T22:46:09Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-512c39663791457d966f0a7b6a3f3fc02023-12-17T06:39:36ZengElsevierAquaculture Reports2352-51342023-12-0133101767Assessing the feasibility of exogenous 17β-estradiol for inducing sex change in white snook, C. viridis: From growth, resting and maturation studiesNavarro-Flores Jaime0Martínez-Brown Juan Manuel1Zavala-Leal Iram2Rojo-Cebreros Angel Humberto3Ibarra-Castro Leonardo4Centro de Investigación en Alimentación y Desarrollo (CIAD) A. C., Unidad Mazatlán, Avenida Sábalo Cerritos S/N, A.P. 711, Mazatlán C.P. 82010, Sinaloa, Mexico; Doctorado en Ciencias Biológico Agropecuarias, Universidad Autónoma de Nayarit, Unidad Académica de Agricultura, Km. 9 Carretera Tepic-Compostela, Xalisco, Nayarit, C.P. 63780, Mexico; Instituto de Ecología, Pesquerías y Oceanografía del Golfo de México, Universidad Autónoma de Campeche (EPOMEX-UAC), Campus VI de Investigaciones, 24030 San Francisco de Campeche, Campeche, MexicoCentro de Investigación en Alimentación y Desarrollo (CIAD) A. C., Unidad Mazatlán, Avenida Sábalo Cerritos S/N, A.P. 711, Mazatlán C.P. 82010, Sinaloa, Mexico; Dirección de Cátedras-CONACYT, Consejo Nacional de Ciencia y Tecnología (CONACYT), Mexico City, MexicoDoctorado en Ciencias Biológico Agropecuarias, Universidad Autónoma de Nayarit, Unidad Académica de Agricultura, Km. 9 Carretera Tepic-Compostela, Xalisco, Nayarit, C.P. 63780, Mexico; Escuela Nacional de Ingeniería Pesquera, Universidad Autónoma de Nayarit, México, Carretera Los Cocos, Bahía de Matanchen, San Blas, Nayarit 63740, MexicoCentro de Investigación en Alimentación y Desarrollo (CIAD) A. C., Unidad Mazatlán, Avenida Sábalo Cerritos S/N, A.P. 711, Mazatlán C.P. 82010, Sinaloa, Mexico; Facultad de Ciencias, Unidad Multidisciplinaria de Docencia e Investigación, Facultad de Ciencias, UNAM, Puerto de Abrigo s/n, Sisal 97356, Mpo., Hunucma, Yucatán, MexicoCentro de Investigación en Alimentación y Desarrollo (CIAD) A. C., Unidad Mazatlán, Avenida Sábalo Cerritos S/N, A.P. 711, Mazatlán C.P. 82010, Sinaloa, Mexico; Program in Fisheries and Aquatic Sciences, School of Forest, Fisheries, and Geomatics Sciences, Institute of Food and Agricultural Sciences, University of Florida, 7922 Northwest 71st Street, Post Office Box 110600, Gainesville, Florida 32611, USA; Whitney Laboratory for Marine Bioscience; 9505 Oceanshore Blvd, St Augustine, Florida, 32080, USA; Corresponding author at: Program in Fisheries and Aquatic Sciences, School of Forest, Fisheries, and Geomatics Sciences, Institute of Food and Agricultural Sciences, University of Florida, 7922 Northwest 71st Street, Post Office Box 110600, Gainesville, Florida 32611, USA.This study evaluated growth, survival, and oogenesis before and after 17β-estradiol (E2) treatments. During the growth period, C. viridis reached a total length of 65 ± 4.2 cm and a weight of 2.5 ± 0.66 kg BW. To induce sex change in males, we implanted silastic tubing loaded with 0 (control), 0.5, or 1.5 mg of E2 kg−1, at days 0, 30, 60, and 90. Blood samples were collected before treatment (day 0) and on days 30, 60, 90, and 120. Initially, 23% of the selected males expressed milt. At 60 days post-treatment 13% in the control and 4.3% in both, 0.5 and 1.5 mg E2 kg−1 BW treatments. After 120 days we observed histological evidence of sex change in 50% of the histology-tagged fish in the 1.5 mg E2 kg−1 treatment. The gonadosomatic index and condition factor did not differ significantly (P > 0.05) between the control and the treatments. However, the mean individual fish weight differed significantly across all sampling times (P < 0.05). The control and 1.5 treatment groups had a 100% survival rate, while the 0.5 treatment showed a 95.6% survival rate. Steroid profiling showed high cortisol levels throughout the experiment but there was no relationship with E2 treatment or sampling point. After the first E2 dose, we observed inhibition of 11-ketotestosterone (11-KT), which persisted until the end of the experiment. At the end of a 150-day rest period, biopsy samples from the fish showed 0%, 29%, and 87% of females with primary growth oocytes in the control, 0.5, and 1.5 treatments, respectively. After 353 days of the maturation period, feminized fish did not reach vitellogenesis. The availability of C. viridis vitellogenic females after induced sex change remains unpredictable. More research is needed to encourage vitellogenesis in hormonally feminized fish of this species.http://www.sciencedirect.com/science/article/pii/S235251342300306XSex changeHermaphroditismGonad histologyVitellogenesisPrecocious female |
| spellingShingle | Navarro-Flores Jaime Martínez-Brown Juan Manuel Zavala-Leal Iram Rojo-Cebreros Angel Humberto Ibarra-Castro Leonardo Assessing the feasibility of exogenous 17β-estradiol for inducing sex change in white snook, C. viridis: From growth, resting and maturation studies Aquaculture Reports Sex change Hermaphroditism Gonad histology Vitellogenesis Precocious female |
| title | Assessing the feasibility of exogenous 17β-estradiol for inducing sex change in white snook, C. viridis: From growth, resting and maturation studies |
| title_full | Assessing the feasibility of exogenous 17β-estradiol for inducing sex change in white snook, C. viridis: From growth, resting and maturation studies |
| title_fullStr | Assessing the feasibility of exogenous 17β-estradiol for inducing sex change in white snook, C. viridis: From growth, resting and maturation studies |
| title_full_unstemmed | Assessing the feasibility of exogenous 17β-estradiol for inducing sex change in white snook, C. viridis: From growth, resting and maturation studies |
| title_short | Assessing the feasibility of exogenous 17β-estradiol for inducing sex change in white snook, C. viridis: From growth, resting and maturation studies |
| title_sort | assessing the feasibility of exogenous 17β estradiol for inducing sex change in white snook c viridis from growth resting and maturation studies |
| topic | Sex change Hermaphroditism Gonad histology Vitellogenesis Precocious female |
| url | http://www.sciencedirect.com/science/article/pii/S235251342300306X |
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