Molecular Landscape of the Epithelial–Mesenchymal Transition in Endometrioid Endometrial Cancer
Modern diagnostics are based on molecular analysis and have been focused on searching for new molecular markers to use in diagnostics. Included in this has been the search for the correlation between gene expression in tissue samples and liquid biological materials. The aim of this study was to eval...
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MDPI AG
2021-04-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/10/7/1520 |
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| author | Marcin Opławski Robert Nowakowski Agata Średnicka Dominika Ochnik Beniamin Oskar Grabarek Dariusz Boroń |
| author_facet | Marcin Opławski Robert Nowakowski Agata Średnicka Dominika Ochnik Beniamin Oskar Grabarek Dariusz Boroń |
| author_sort | Marcin Opławski |
| collection | DOAJ |
| description | Modern diagnostics are based on molecular analysis and have been focused on searching for new molecular markers to use in diagnostics. Included in this has been the search for the correlation between gene expression in tissue samples and liquid biological materials. The aim of this study was to evaluate the differences in the expression profile of messenger RNA (mRNA) and micro-RNA (miRNA) related to the epithelial–mesenchymal transition (EMT) in different grades of endometrial cancer (G1–G3), in order to select the most promising molecular markers. The study material consisted of tissue samples and whole blood collected from 30 patients with endometrial cancer (study group; G1 = 15; G2 = 8; G3 = 7) and 30 without neoplastic changes (control group). The molecular analysis included the use of the microarray technique and RTqPCR. Microarray analysis indicated the following number of mRNA differentiating the endometrial cancer samples from the control (tissue/blood): G1 vs. C = 21/18 mRNAs, G2 vs. C = 19/14 mRNAs, and G3 vs. C = 10/9 mRNAs. The common genes for the tissue and blood samples (Fold Change; FC > 3.0) were G1 vs. C: <i>TGFB1</i>, <i>WNT5A</i>, <i>TGFB2</i>, and <i>NOTCH1</i>; G2 vs. C: <i>BCL2L</i>, <i>SOX9</i>, <i>BAMBI</i>, and <i>SMAD4</i>; G3 vs. C <i>STAT1</i> and <i>TGFB1</i>. In addition, mRNA <i>TGFB1</i>, <i>NOTCH1</i>, and <i>BCL2L</i> are common for all grades of endometrial cancer. The analysis showed that miR-144, miR-106a, and miR-30d are most strongly associated with EMT, making them potential diagnostic markers. |
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| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T12:34:44Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-512da3d8186c4dda9cd63e0b95f693dd2023-11-21T14:19:31ZengMDPI AGJournal of Clinical Medicine2077-03832021-04-01107152010.3390/jcm10071520Molecular Landscape of the Epithelial–Mesenchymal Transition in Endometrioid Endometrial CancerMarcin Opławski0Robert Nowakowski1Agata Średnicka2Dominika Ochnik3Beniamin Oskar Grabarek4Dariusz Boroń5Department of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology in Katowice, 41-800 Zabrze, PolandDepartment of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology in Katowice, 41-800 Zabrze, PolandDepartment of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, PolandDepartment of Gynecology and Obstetrics with Gynecologic Oncology, Ludwik Rydygier Memorial Specialized Hospital, 31-826 Kraków, PolandModern diagnostics are based on molecular analysis and have been focused on searching for new molecular markers to use in diagnostics. Included in this has been the search for the correlation between gene expression in tissue samples and liquid biological materials. The aim of this study was to evaluate the differences in the expression profile of messenger RNA (mRNA) and micro-RNA (miRNA) related to the epithelial–mesenchymal transition (EMT) in different grades of endometrial cancer (G1–G3), in order to select the most promising molecular markers. The study material consisted of tissue samples and whole blood collected from 30 patients with endometrial cancer (study group; G1 = 15; G2 = 8; G3 = 7) and 30 without neoplastic changes (control group). The molecular analysis included the use of the microarray technique and RTqPCR. Microarray analysis indicated the following number of mRNA differentiating the endometrial cancer samples from the control (tissue/blood): G1 vs. C = 21/18 mRNAs, G2 vs. C = 19/14 mRNAs, and G3 vs. C = 10/9 mRNAs. The common genes for the tissue and blood samples (Fold Change; FC > 3.0) were G1 vs. C: <i>TGFB1</i>, <i>WNT5A</i>, <i>TGFB2</i>, and <i>NOTCH1</i>; G2 vs. C: <i>BCL2L</i>, <i>SOX9</i>, <i>BAMBI</i>, and <i>SMAD4</i>; G3 vs. C <i>STAT1</i> and <i>TGFB1</i>. In addition, mRNA <i>TGFB1</i>, <i>NOTCH1</i>, and <i>BCL2L</i> are common for all grades of endometrial cancer. The analysis showed that miR-144, miR-106a, and miR-30d are most strongly associated with EMT, making them potential diagnostic markers.https://www.mdpi.com/2077-0383/10/7/1520endometrial cancerepithelial–mesenchymal transitiontissueliquid biopsymiRNAmRNA |
| spellingShingle | Marcin Opławski Robert Nowakowski Agata Średnicka Dominika Ochnik Beniamin Oskar Grabarek Dariusz Boroń Molecular Landscape of the Epithelial–Mesenchymal Transition in Endometrioid Endometrial Cancer Journal of Clinical Medicine endometrial cancer epithelial–mesenchymal transition tissue liquid biopsy miRNA mRNA |
| title | Molecular Landscape of the Epithelial–Mesenchymal Transition in Endometrioid Endometrial Cancer |
| title_full | Molecular Landscape of the Epithelial–Mesenchymal Transition in Endometrioid Endometrial Cancer |
| title_fullStr | Molecular Landscape of the Epithelial–Mesenchymal Transition in Endometrioid Endometrial Cancer |
| title_full_unstemmed | Molecular Landscape of the Epithelial–Mesenchymal Transition in Endometrioid Endometrial Cancer |
| title_short | Molecular Landscape of the Epithelial–Mesenchymal Transition in Endometrioid Endometrial Cancer |
| title_sort | molecular landscape of the epithelial mesenchymal transition in endometrioid endometrial cancer |
| topic | endometrial cancer epithelial–mesenchymal transition tissue liquid biopsy miRNA mRNA |
| url | https://www.mdpi.com/2077-0383/10/7/1520 |
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