CircAgtpbp1 Acts as a Molecular Sponge of miR-543-5p to Regulate the Secretion of GH in Rat Pituitary Cells
CircRNAs have been identified to be expressed differently and stably in numerous species and tissues, but their functions in growth hormone (GH) secretion are still largely unknown. In summary, we have revealed a circRNA-miRNA-mRNA network that may play a biological role in the rat pituitary gland....
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MDPI AG
2021-02-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/2/558 |
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| author | ZeWen Yu WenZhi Ren Tian Wang WeiDi Zhang ChangJiang Wang HaoQi Wang Fei Gao Bao Yuan |
| author_facet | ZeWen Yu WenZhi Ren Tian Wang WeiDi Zhang ChangJiang Wang HaoQi Wang Fei Gao Bao Yuan |
| author_sort | ZeWen Yu |
| collection | DOAJ |
| description | CircRNAs have been identified to be expressed differently and stably in numerous species and tissues, but their functions in growth hormone (GH) secretion are still largely unknown. In summary, we have revealed a circRNA-miRNA-mRNA network that may play a biological role in the rat pituitary gland. First, we verified the chromosome location information of circAgtpbp1 according to sequencing analysis. The circAgtpbp1 characteristics were authenticated through PCR, qRT–PCR, treating with RNase and fluorescent in situ hybridization (FISH). Second, we detected the expression pattern of circAgtpbp1 in the rat anterior pituitary by qRT–PCR. We also designed circAgtpbp1 siRNA and constructed overexpression plasmid to evaluate the effect of circAgtpbp1 function on GH secretion by qRT–PCR, ELISA and Western blot. CircAgtpbp1 is a stable, truly circular molecule. We found that circAgtpbp1 interacted with miR-543-5p and can regulate GH secretion in pituitary cells through a circAgtpbp1-miR-543-5p-GH axis. Overall, the evidence generated by our study suggests that circAgtpbp1 can act as a sponge of miR-543-5p to reduce the inhibitory effect of miR-543-5p on Gh1 and further promote GH secretion. These findings expand our existing knowledge on the mechanisms of hormone regulation in the pituitary gland. |
| first_indexed | 2024-03-09T00:41:32Z |
| format | Article |
| id | doaj.art-512e481aa08a4fcdb6ab0d7ec612fd57 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T00:41:32Z |
| publishDate | 2021-02-01 |
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| spelling | doaj.art-512e481aa08a4fcdb6ab0d7ec612fd572023-12-11T17:46:52ZengMDPI AGAnimals2076-26152021-02-0111255810.3390/ani11020558CircAgtpbp1 Acts as a Molecular Sponge of miR-543-5p to Regulate the Secretion of GH in Rat Pituitary CellsZeWen Yu0WenZhi Ren1Tian Wang2WeiDi Zhang3ChangJiang Wang4HaoQi Wang5Fei Gao6Bao Yuan7Department of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi’an Road, Changchun 130062, ChinaDepartment of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi’an Road, Changchun 130062, ChinaDepartment of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi’an Road, Changchun 130062, ChinaDepartment of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi’an Road, Changchun 130062, ChinaDepartment of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi’an Road, Changchun 130062, ChinaDepartment of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi’an Road, Changchun 130062, ChinaDepartment of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi’an Road, Changchun 130062, ChinaDepartment of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi’an Road, Changchun 130062, ChinaCircRNAs have been identified to be expressed differently and stably in numerous species and tissues, but their functions in growth hormone (GH) secretion are still largely unknown. In summary, we have revealed a circRNA-miRNA-mRNA network that may play a biological role in the rat pituitary gland. First, we verified the chromosome location information of circAgtpbp1 according to sequencing analysis. The circAgtpbp1 characteristics were authenticated through PCR, qRT–PCR, treating with RNase and fluorescent in situ hybridization (FISH). Second, we detected the expression pattern of circAgtpbp1 in the rat anterior pituitary by qRT–PCR. We also designed circAgtpbp1 siRNA and constructed overexpression plasmid to evaluate the effect of circAgtpbp1 function on GH secretion by qRT–PCR, ELISA and Western blot. CircAgtpbp1 is a stable, truly circular molecule. We found that circAgtpbp1 interacted with miR-543-5p and can regulate GH secretion in pituitary cells through a circAgtpbp1-miR-543-5p-GH axis. Overall, the evidence generated by our study suggests that circAgtpbp1 can act as a sponge of miR-543-5p to reduce the inhibitory effect of miR-543-5p on Gh1 and further promote GH secretion. These findings expand our existing knowledge on the mechanisms of hormone regulation in the pituitary gland.https://www.mdpi.com/2076-2615/11/2/558circRNAmiRNA spongeGHpituitaryanimal growth |
| spellingShingle | ZeWen Yu WenZhi Ren Tian Wang WeiDi Zhang ChangJiang Wang HaoQi Wang Fei Gao Bao Yuan CircAgtpbp1 Acts as a Molecular Sponge of miR-543-5p to Regulate the Secretion of GH in Rat Pituitary Cells Animals circRNA miRNA sponge GH pituitary animal growth |
| title | CircAgtpbp1 Acts as a Molecular Sponge of miR-543-5p to Regulate the Secretion of GH in Rat Pituitary Cells |
| title_full | CircAgtpbp1 Acts as a Molecular Sponge of miR-543-5p to Regulate the Secretion of GH in Rat Pituitary Cells |
| title_fullStr | CircAgtpbp1 Acts as a Molecular Sponge of miR-543-5p to Regulate the Secretion of GH in Rat Pituitary Cells |
| title_full_unstemmed | CircAgtpbp1 Acts as a Molecular Sponge of miR-543-5p to Regulate the Secretion of GH in Rat Pituitary Cells |
| title_short | CircAgtpbp1 Acts as a Molecular Sponge of miR-543-5p to Regulate the Secretion of GH in Rat Pituitary Cells |
| title_sort | circagtpbp1 acts as a molecular sponge of mir 543 5p to regulate the secretion of gh in rat pituitary cells |
| topic | circRNA miRNA sponge GH pituitary animal growth |
| url | https://www.mdpi.com/2076-2615/11/2/558 |
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