Purification and Identification of Novel Antioxidant Peptides Isolated from <i>Geoffroea decorticans</i> Seeds with Anticoagulant Activity
<i>Geoffroea decorticans</i> is a xerophilous deciduous tree present in most arid forests of southern South America, which is commonly used in traditional medicine. The seeds of this tree have been previously investigated for their singular chemical composition, but their protein content...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/8/1153 |
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| author | Juliana Cotabarren Brenda Ozón Santiago Claver Javier Garcia-Pardo Walter David Obregón |
| author_facet | Juliana Cotabarren Brenda Ozón Santiago Claver Javier Garcia-Pardo Walter David Obregón |
| author_sort | Juliana Cotabarren |
| collection | DOAJ |
| description | <i>Geoffroea decorticans</i> is a xerophilous deciduous tree present in most arid forests of southern South America, which is commonly used in traditional medicine. The seeds of this tree have been previously investigated for their singular chemical composition, but their protein content has been poorly investigated. Herein, we report the isolation, purification, and characterization of a set of thermostable peptides derived from <i>Geoffroea decorticans</i> seeds (GdAPs) with strong antioxidant and anticoagulant activities. The most potent antioxidant peptides showed a half maximal inhibitory concentration (IC<sub>50</sub>) of 35.5 ± 0.3 µg/mL determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH). They also caused a dose-dependent prolongation of the aPTT clotting time with an IC<sub>50</sub> value of ~82 µg/mL. Interestingly, MALDI-TOF/MS analysis showed the presence of three major peptides with low molecular weights of 2257.199 Da, 2717.165 Da, and 5422.002 Da. The derived amino-acid sequence of GdAPs revealed their unique structural features, exhibiting homology with various proteins present in the genome of <i>Arachis hypogaea</i>. All in all, our data suggest a direct applicability of GdAPs for pharmaceutical purposes. |
| first_indexed | 2024-03-10T08:29:11Z |
| format | Article |
| id | doaj.art-512e6a5ceabc4de7a106e7a7526afe0d |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T08:29:11Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-512e6a5ceabc4de7a106e7a7526afe0d2023-11-22T09:13:25ZengMDPI AGPharmaceutics1999-49232021-07-01138115310.3390/pharmaceutics13081153Purification and Identification of Novel Antioxidant Peptides Isolated from <i>Geoffroea decorticans</i> Seeds with Anticoagulant ActivityJuliana Cotabarren0Brenda Ozón1Santiago Claver2Javier Garcia-Pardo3Walter David Obregón4Centro de Investigación de Proteínas Vegetales (CIPROVE), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de la Plata, Buenos Aires B1900, ArgentinaCentro de Investigación de Proteínas Vegetales (CIPROVE), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de la Plata, Buenos Aires B1900, ArgentinaCentro de Investigación de Proteínas Vegetales (CIPROVE), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de la Plata, Buenos Aires B1900, ArgentinaInstitut de Biotecnologia i Biomedicina and Departament de Bioquimica i Biologia Molecular, Universitat Autònoma de Barcelona, 08193 Barcelona, SpainCentro de Investigación de Proteínas Vegetales (CIPROVE), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de la Plata, Buenos Aires B1900, Argentina<i>Geoffroea decorticans</i> is a xerophilous deciduous tree present in most arid forests of southern South America, which is commonly used in traditional medicine. The seeds of this tree have been previously investigated for their singular chemical composition, but their protein content has been poorly investigated. Herein, we report the isolation, purification, and characterization of a set of thermostable peptides derived from <i>Geoffroea decorticans</i> seeds (GdAPs) with strong antioxidant and anticoagulant activities. The most potent antioxidant peptides showed a half maximal inhibitory concentration (IC<sub>50</sub>) of 35.5 ± 0.3 µg/mL determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH). They also caused a dose-dependent prolongation of the aPTT clotting time with an IC<sub>50</sub> value of ~82 µg/mL. Interestingly, MALDI-TOF/MS analysis showed the presence of three major peptides with low molecular weights of 2257.199 Da, 2717.165 Da, and 5422.002 Da. The derived amino-acid sequence of GdAPs revealed their unique structural features, exhibiting homology with various proteins present in the genome of <i>Arachis hypogaea</i>. All in all, our data suggest a direct applicability of GdAPs for pharmaceutical purposes.https://www.mdpi.com/1999-4923/13/8/1153<i>Geoffroea decorticans</i>antioxidantfunctional compoundthermostable proteinreactive oxygen speciesanticoagulant |
| spellingShingle | Juliana Cotabarren Brenda Ozón Santiago Claver Javier Garcia-Pardo Walter David Obregón Purification and Identification of Novel Antioxidant Peptides Isolated from <i>Geoffroea decorticans</i> Seeds with Anticoagulant Activity Pharmaceutics <i>Geoffroea decorticans</i> antioxidant functional compound thermostable protein reactive oxygen species anticoagulant |
| title | Purification and Identification of Novel Antioxidant Peptides Isolated from <i>Geoffroea decorticans</i> Seeds with Anticoagulant Activity |
| title_full | Purification and Identification of Novel Antioxidant Peptides Isolated from <i>Geoffroea decorticans</i> Seeds with Anticoagulant Activity |
| title_fullStr | Purification and Identification of Novel Antioxidant Peptides Isolated from <i>Geoffroea decorticans</i> Seeds with Anticoagulant Activity |
| title_full_unstemmed | Purification and Identification of Novel Antioxidant Peptides Isolated from <i>Geoffroea decorticans</i> Seeds with Anticoagulant Activity |
| title_short | Purification and Identification of Novel Antioxidant Peptides Isolated from <i>Geoffroea decorticans</i> Seeds with Anticoagulant Activity |
| title_sort | purification and identification of novel antioxidant peptides isolated from i geoffroea decorticans i seeds with anticoagulant activity |
| topic | <i>Geoffroea decorticans</i> antioxidant functional compound thermostable protein reactive oxygen species anticoagulant |
| url | https://www.mdpi.com/1999-4923/13/8/1153 |
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