In Vitro Techniques and Measurements of Phage Characteristics That Are Important for Phage Therapy Success
Validated methods for phage selection, host range expansion, and lytic activity determination are indispensable for maximizing phage therapy outcomes. In this review, we describe some relevant methods, highlighting their advantages and disadvantages, and categorize them as preliminary or confirmator...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/7/1490 |
| _version_ | 1797433036578488320 |
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| author | Tea Glonti Jean-Paul Pirnay |
| author_facet | Tea Glonti Jean-Paul Pirnay |
| author_sort | Tea Glonti |
| collection | DOAJ |
| description | Validated methods for phage selection, host range expansion, and lytic activity determination are indispensable for maximizing phage therapy outcomes. In this review, we describe some relevant methods, highlighting their advantages and disadvantages, and categorize them as preliminary or confirmatory methods where appropriate. Experimental conditions, such as the composition and consistency of culture media, have an impact on bacterial growth and, consequently, phage propagation and the selection of phage-resistant mutants. The phages require different experimental conditions to be tested to fully reveal their characteristics and phage therapy potential in view of their future use in therapy. Phage lytic activity or virulence should be considered as a result of the phage, its host, and intracellular/environmental factors, including the ability of a phage to recognize receptors on the bacterial cell surface. In vitro quantitative and qualitative measurements of phage characteristics, further validated by in vivo experiments, could be incorporated into one system or mathematical model/formula, which could predict a potential successful outcome of clinical applications. |
| first_indexed | 2024-03-09T10:10:20Z |
| format | Article |
| id | doaj.art-512f78cac9a0416cae304f636a890803 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T10:10:20Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-512f78cac9a0416cae304f636a8908032023-12-01T22:47:14ZengMDPI AGViruses1999-49152022-07-01147149010.3390/v14071490In Vitro Techniques and Measurements of Phage Characteristics That Are Important for Phage Therapy SuccessTea Glonti0Jean-Paul Pirnay1Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, B-1120 Brussels, BelgiumLaboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, B-1120 Brussels, BelgiumValidated methods for phage selection, host range expansion, and lytic activity determination are indispensable for maximizing phage therapy outcomes. In this review, we describe some relevant methods, highlighting their advantages and disadvantages, and categorize them as preliminary or confirmatory methods where appropriate. Experimental conditions, such as the composition and consistency of culture media, have an impact on bacterial growth and, consequently, phage propagation and the selection of phage-resistant mutants. The phages require different experimental conditions to be tested to fully reveal their characteristics and phage therapy potential in view of their future use in therapy. Phage lytic activity or virulence should be considered as a result of the phage, its host, and intracellular/environmental factors, including the ability of a phage to recognize receptors on the bacterial cell surface. In vitro quantitative and qualitative measurements of phage characteristics, further validated by in vivo experiments, could be incorporated into one system or mathematical model/formula, which could predict a potential successful outcome of clinical applications.https://www.mdpi.com/1999-4915/14/7/1490phage isolationphage selectionphage virulencephage activityphage therapy |
| spellingShingle | Tea Glonti Jean-Paul Pirnay In Vitro Techniques and Measurements of Phage Characteristics That Are Important for Phage Therapy Success Viruses phage isolation phage selection phage virulence phage activity phage therapy |
| title | In Vitro Techniques and Measurements of Phage Characteristics That Are Important for Phage Therapy Success |
| title_full | In Vitro Techniques and Measurements of Phage Characteristics That Are Important for Phage Therapy Success |
| title_fullStr | In Vitro Techniques and Measurements of Phage Characteristics That Are Important for Phage Therapy Success |
| title_full_unstemmed | In Vitro Techniques and Measurements of Phage Characteristics That Are Important for Phage Therapy Success |
| title_short | In Vitro Techniques and Measurements of Phage Characteristics That Are Important for Phage Therapy Success |
| title_sort | in vitro techniques and measurements of phage characteristics that are important for phage therapy success |
| topic | phage isolation phage selection phage virulence phage activity phage therapy |
| url | https://www.mdpi.com/1999-4915/14/7/1490 |
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