Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo tests
Abstract Background Several chemical bactericides were applied for controlling soft rot bacteria, Pectobacterium carotovorum subsp. carotovorum, which causes the destructive soft rot disease to many economically important vegetables, but because of their toxic hazards on human and environment became...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-02-01
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| Series: | Bulletin of the National Research Centre |
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| Online Access: | https://doi.org/10.1186/s42269-021-00491-4 |
| _version_ | 1818453686611869696 |
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| author | Hassan Abd-El-Khair Tarek G. Abdel-Gaied Maurice S. Mikhail Ahmed I. Abdel-Alim Hamdy I. Seif El-Nasr |
| author_facet | Hassan Abd-El-Khair Tarek G. Abdel-Gaied Maurice S. Mikhail Ahmed I. Abdel-Alim Hamdy I. Seif El-Nasr |
| author_sort | Hassan Abd-El-Khair |
| collection | DOAJ |
| description | Abstract Background Several chemical bactericides were applied for controlling soft rot bacteria, Pectobacterium carotovorum subsp. carotovorum, which causes the destructive soft rot disease to many economically important vegetables, but because of their toxic hazards on human and environment became limit. The biocontrol was applied to control many plant pathogens. Therefore, this work is aimed to study the antagonistic activity of bacterial agents, i.e. Bacillus subtilis, Bacillus pumilus, Bacillus megaterium and Pseudomonas fluorescens, and fugal agents, i.e. Trichoderma harzianum, Trichoderma viride and Trichoderma virens, to control bacterial soft rot disease under in vitro and in vivo tests. Results The tested treatments could protect the potato tubers against the development of soft rot. T. viride and T. virens were highly effective in reducing soft rot symptoms on inoculated potato tuber slices, when applied at the same time or 2 h before pathogen inoculation, while B. megaterium and T. harzianum were highly effective when applied at the same time or 2 h after pathogen inoculation. In whole potato tubers technique, B.pumilus highly protected the stored potato tuber under artificially infection conditions, than P. fluorescens, T. harzianum, B. subtilis, T. viride, T. virens and B. megaterium, respectively. Conclusion Application of fungal agents or specify the bacterial species can play an important role in controlling bacterial soft rot disease in vegetables and increase the stored periods of potato tubers under storage conditions without any toxic effects. |
| first_indexed | 2024-12-14T21:42:56Z |
| format | Article |
| id | doaj.art-51324f8f2c854b7eb57f18e3c1585d5f |
| institution | Directory Open Access Journal |
| issn | 2522-8307 |
| language | English |
| last_indexed | 2024-12-14T21:42:56Z |
| publishDate | 2021-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bulletin of the National Research Centre |
| spelling | doaj.art-51324f8f2c854b7eb57f18e3c1585d5f2022-12-21T22:46:24ZengSpringerOpenBulletin of the National Research Centre2522-83072021-02-014511910.1186/s42269-021-00491-4Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo testsHassan Abd-El-Khair0Tarek G. Abdel-Gaied1Maurice S. Mikhail2Ahmed I. Abdel-Alim3Hamdy I. Seif El-Nasr4Plant Pathology Department, National Research CentrePlant Pathology Department, National Research CentrePlant Pathology Department, Faculty of Agriculture, Cairo UniversityPlant Pathology Department, Faculty of Agriculture, Cairo UniversityPlant Pathology Department, National Research CentreAbstract Background Several chemical bactericides were applied for controlling soft rot bacteria, Pectobacterium carotovorum subsp. carotovorum, which causes the destructive soft rot disease to many economically important vegetables, but because of their toxic hazards on human and environment became limit. The biocontrol was applied to control many plant pathogens. Therefore, this work is aimed to study the antagonistic activity of bacterial agents, i.e. Bacillus subtilis, Bacillus pumilus, Bacillus megaterium and Pseudomonas fluorescens, and fugal agents, i.e. Trichoderma harzianum, Trichoderma viride and Trichoderma virens, to control bacterial soft rot disease under in vitro and in vivo tests. Results The tested treatments could protect the potato tubers against the development of soft rot. T. viride and T. virens were highly effective in reducing soft rot symptoms on inoculated potato tuber slices, when applied at the same time or 2 h before pathogen inoculation, while B. megaterium and T. harzianum were highly effective when applied at the same time or 2 h after pathogen inoculation. In whole potato tubers technique, B.pumilus highly protected the stored potato tuber under artificially infection conditions, than P. fluorescens, T. harzianum, B. subtilis, T. viride, T. virens and B. megaterium, respectively. Conclusion Application of fungal agents or specify the bacterial species can play an important role in controlling bacterial soft rot disease in vegetables and increase the stored periods of potato tubers under storage conditions without any toxic effects.https://doi.org/10.1186/s42269-021-00491-4Biological controlPectobacterium carotovorumSoft rot diseaseVegetables |
| spellingShingle | Hassan Abd-El-Khair Tarek G. Abdel-Gaied Maurice S. Mikhail Ahmed I. Abdel-Alim Hamdy I. Seif El-Nasr Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo tests Bulletin of the National Research Centre Biological control Pectobacterium carotovorum Soft rot disease Vegetables |
| title | Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo tests |
| title_full | Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo tests |
| title_fullStr | Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo tests |
| title_full_unstemmed | Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo tests |
| title_short | Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo tests |
| title_sort | biological control of pectobacterium carotovorum subsp carotovorum the causal agent of bacterial soft rot in vegetables in vitro and in vivo tests |
| topic | Biological control Pectobacterium carotovorum Soft rot disease Vegetables |
| url | https://doi.org/10.1186/s42269-021-00491-4 |
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