Multiple clades of Husavirus in South America revealed by next generation sequencing.
Husavirus (HuV) is an unclassified virus of the order Picornavirales that has already been identified worldwide in various locations. The genetic, epidemiological, and pathogenic characteristics are, however, little understood. In children with acute gastroenteritis, this study used next-generation...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0248486 |
| _version_ | 1819199421478338560 |
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| author | Endrya do Socorro Fôro Ramos Ulisses Alves Rosa Geovani de Oliveira Ribeiro Fabiola Villanova Flávio Augusto de Pádua Milagres Rafael Brustulin Vanessa Dos Santos Morais Emerson Luiz Lima Araújo Ramendra Pati Pandey V Samuel Raj Ester Cerdeira Sabino Xutao Deng Eric Delwart Adriana Luchs Élcio Leal Antonio Charlys da Costa |
| author_facet | Endrya do Socorro Fôro Ramos Ulisses Alves Rosa Geovani de Oliveira Ribeiro Fabiola Villanova Flávio Augusto de Pádua Milagres Rafael Brustulin Vanessa Dos Santos Morais Emerson Luiz Lima Araújo Ramendra Pati Pandey V Samuel Raj Ester Cerdeira Sabino Xutao Deng Eric Delwart Adriana Luchs Élcio Leal Antonio Charlys da Costa |
| author_sort | Endrya do Socorro Fôro Ramos |
| collection | DOAJ |
| description | Husavirus (HuV) is an unclassified virus of the order Picornavirales that has already been identified worldwide in various locations. The genetic, epidemiological, and pathogenic characteristics are, however, little understood. In children with acute gastroenteritis, this study used next-generation sequencing to recognize unknown sources of viruses. In particular, 251 fecal samples obtained from individuals were sequenced in southern, northeastern, and northern Brazil. all samples were also analyzed using culture methods and parasitological tests to classify other enteric pathogens such as bacteria, parasites, and viruses. 1.9% of the samples tested positive for HuV, for a total of 5 positive children, with a mean age of 2 year, with three males and two females. Detailed molecular characterization of full genomes showed that Brazilian HuVs' nucleotide divergence is less than 11%. The genetic gap between Brazilian sequences and the closest HuV reported previously, on the other hand, is 18%. The study showed that Brazilian sequences are closely related to the HuV defined in Viet Nam in 2013, further characterization based on phylogenetics. At least two divergent clades of HuV in South America were also seen in the phylogenetic study. |
| first_indexed | 2024-12-23T03:16:04Z |
| format | Article |
| id | doaj.art-513424264bd8471dad26283d1ab765a5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T03:16:04Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-513424264bd8471dad26283d1ab765a52022-12-21T18:02:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01163e024848610.1371/journal.pone.0248486Multiple clades of Husavirus in South America revealed by next generation sequencing.Endrya do Socorro Fôro RamosUlisses Alves RosaGeovani de Oliveira RibeiroFabiola VillanovaFlávio Augusto de Pádua MilagresRafael BrustulinVanessa Dos Santos MoraisEmerson Luiz Lima AraújoRamendra Pati PandeyV Samuel RajEster Cerdeira SabinoXutao DengEric DelwartAdriana LuchsÉlcio LealAntonio Charlys da CostaHusavirus (HuV) is an unclassified virus of the order Picornavirales that has already been identified worldwide in various locations. The genetic, epidemiological, and pathogenic characteristics are, however, little understood. In children with acute gastroenteritis, this study used next-generation sequencing to recognize unknown sources of viruses. In particular, 251 fecal samples obtained from individuals were sequenced in southern, northeastern, and northern Brazil. all samples were also analyzed using culture methods and parasitological tests to classify other enteric pathogens such as bacteria, parasites, and viruses. 1.9% of the samples tested positive for HuV, for a total of 5 positive children, with a mean age of 2 year, with three males and two females. Detailed molecular characterization of full genomes showed that Brazilian HuVs' nucleotide divergence is less than 11%. The genetic gap between Brazilian sequences and the closest HuV reported previously, on the other hand, is 18%. The study showed that Brazilian sequences are closely related to the HuV defined in Viet Nam in 2013, further characterization based on phylogenetics. At least two divergent clades of HuV in South America were also seen in the phylogenetic study.https://doi.org/10.1371/journal.pone.0248486 |
| spellingShingle | Endrya do Socorro Fôro Ramos Ulisses Alves Rosa Geovani de Oliveira Ribeiro Fabiola Villanova Flávio Augusto de Pádua Milagres Rafael Brustulin Vanessa Dos Santos Morais Emerson Luiz Lima Araújo Ramendra Pati Pandey V Samuel Raj Ester Cerdeira Sabino Xutao Deng Eric Delwart Adriana Luchs Élcio Leal Antonio Charlys da Costa Multiple clades of Husavirus in South America revealed by next generation sequencing. PLoS ONE |
| title | Multiple clades of Husavirus in South America revealed by next generation sequencing. |
| title_full | Multiple clades of Husavirus in South America revealed by next generation sequencing. |
| title_fullStr | Multiple clades of Husavirus in South America revealed by next generation sequencing. |
| title_full_unstemmed | Multiple clades of Husavirus in South America revealed by next generation sequencing. |
| title_short | Multiple clades of Husavirus in South America revealed by next generation sequencing. |
| title_sort | multiple clades of husavirus in south america revealed by next generation sequencing |
| url | https://doi.org/10.1371/journal.pone.0248486 |
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