Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS)
<p>Abstract</p> <p>Objective</p> <p>To immunophenotype CD4<sup>+ </sup>and CD8<sup>+ </sup>T cell sub-populations in HIV-associated immune reconstitution inflammatory syndrome (IRIS).</p> <p>Design</p> <p>Nested case-contr...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-07-01
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| Series: | AIDS Research and Therapy |
| Online Access: | http://www.aidsrestherapy.com/content/6/1/16 |
| _version_ | 1819040958678827008 |
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| author | Glencross Deborah K Van Rie Annelies Feldman Charles Ottinger Janet S Mhlangu Patrick Venter Willem DF Suchard Melinda S Murdoch David M Stevens Wendy S Weinhold Kent J |
| author_facet | Glencross Deborah K Van Rie Annelies Feldman Charles Ottinger Janet S Mhlangu Patrick Venter Willem DF Suchard Melinda S Murdoch David M Stevens Wendy S Weinhold Kent J |
| author_sort | Glencross Deborah K |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Objective</p> <p>To immunophenotype CD4<sup>+ </sup>and CD8<sup>+ </sup>T cell sub-populations in HIV-associated immune reconstitution inflammatory syndrome (IRIS).</p> <p>Design</p> <p>Nested case-control immunological study.</p> <p>Methods</p> <p>ART-naïve HIV-infected patients were prospectively observed for IRIS during the first 6 months of ART. Twenty-two IRIS cases and 22 ART-duration matched controls were sampled for T cell immunophenotyping.</p> <p>Results</p> <p>IRIS cases demonstrated significantly lower CD4 cell counts compared to controls (baseline: 79 versus 142, p = 0.02; enrollment: 183 versus 263, p = 0.05, respectively) with no differences in HIV RNA levels. Within CD4<sup>+</sup>T cells, cases exhibited more of an effector memory phenotype compared to controls (40.8 versus 27.0%, p = 0.20), while controls trended towards a central memory phenotype (43.8 versus 30.8%, p = 0.07). Within CD8<sup>+ </sup>T cells, controls exhibited more central memory (13.9 versus 7.81%, p = 0.01, respectively) and effector (13.2 versus 8.8%, p = 0.04, respectively) phenotypes compared to cases, whereas cases demonstrated more terminal effectors than controls (28.8 versus 15.1%, p = 0.05). Cases demonstrated increased activation of CD8<sup>+ </sup>T cell effector memory, terminal effector, and effector subsets than controls (p = 0.04, 0.02, and 0.02, respectively).</p> <p>Conclusion</p> <p>CD4<sup>+ </sup>and CD8<sup>+ </sup>T cell subset maturational phenotypes were heterogeneous among IRIS cases and controls. However, IRIS cases demonstrated significant increases in activation of CD8<sup>+ </sup>T cell effector subpopulations.</p> |
| first_indexed | 2024-12-21T09:17:22Z |
| format | Article |
| id | doaj.art-5134a5d818354471b8916755d6e92d8e |
| institution | Directory Open Access Journal |
| issn | 1742-6405 |
| language | English |
| last_indexed | 2024-12-21T09:17:22Z |
| publishDate | 2009-07-01 |
| publisher | BMC |
| record_format | Article |
| series | AIDS Research and Therapy |
| spelling | doaj.art-5134a5d818354471b8916755d6e92d8e2022-12-21T19:09:06ZengBMCAIDS Research and Therapy1742-64052009-07-01611610.1186/1742-6405-6-16Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS)Glencross Deborah KVan Rie AnneliesFeldman CharlesOttinger Janet SMhlangu PatrickVenter Willem DFSuchard Melinda SMurdoch David MStevens Wendy SWeinhold Kent J<p>Abstract</p> <p>Objective</p> <p>To immunophenotype CD4<sup>+ </sup>and CD8<sup>+ </sup>T cell sub-populations in HIV-associated immune reconstitution inflammatory syndrome (IRIS).</p> <p>Design</p> <p>Nested case-control immunological study.</p> <p>Methods</p> <p>ART-naïve HIV-infected patients were prospectively observed for IRIS during the first 6 months of ART. Twenty-two IRIS cases and 22 ART-duration matched controls were sampled for T cell immunophenotyping.</p> <p>Results</p> <p>IRIS cases demonstrated significantly lower CD4 cell counts compared to controls (baseline: 79 versus 142, p = 0.02; enrollment: 183 versus 263, p = 0.05, respectively) with no differences in HIV RNA levels. Within CD4<sup>+</sup>T cells, cases exhibited more of an effector memory phenotype compared to controls (40.8 versus 27.0%, p = 0.20), while controls trended towards a central memory phenotype (43.8 versus 30.8%, p = 0.07). Within CD8<sup>+ </sup>T cells, controls exhibited more central memory (13.9 versus 7.81%, p = 0.01, respectively) and effector (13.2 versus 8.8%, p = 0.04, respectively) phenotypes compared to cases, whereas cases demonstrated more terminal effectors than controls (28.8 versus 15.1%, p = 0.05). Cases demonstrated increased activation of CD8<sup>+ </sup>T cell effector memory, terminal effector, and effector subsets than controls (p = 0.04, 0.02, and 0.02, respectively).</p> <p>Conclusion</p> <p>CD4<sup>+ </sup>and CD8<sup>+ </sup>T cell subset maturational phenotypes were heterogeneous among IRIS cases and controls. However, IRIS cases demonstrated significant increases in activation of CD8<sup>+ </sup>T cell effector subpopulations.</p>http://www.aidsrestherapy.com/content/6/1/16 |
| spellingShingle | Glencross Deborah K Van Rie Annelies Feldman Charles Ottinger Janet S Mhlangu Patrick Venter Willem DF Suchard Melinda S Murdoch David M Stevens Wendy S Weinhold Kent J Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS) AIDS Research and Therapy |
| title | Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS) |
| title_full | Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS) |
| title_fullStr | Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS) |
| title_full_unstemmed | Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS) |
| title_short | Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS) |
| title_sort | polychromatic immunophenotypic characterization of t cell profiles among hiv infected patients experiencing immune reconstitution inflammatory syndrome iris |
| url | http://www.aidsrestherapy.com/content/6/1/16 |
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