The Expression of Transcription Factors in Fetal Lamb Kidney
(1) Background: Renal development involves frequent expression and loss of transcription factors, resulting in the activation of genes. Wilms’ tumor 1 (WT1), hepatocyte nuclear factor-1-beta (HNF1β), and paired box genes 2 and 8 (Pax2 and Pax8) play an important role in renal development. With this...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Journal of Developmental Biology |
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| Online Access: | https://www.mdpi.com/2221-3759/9/2/22 |
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| author | Yuri Nishiya Kohei Kawaguchi Kosuke Kudo Takuya Kawaguchi Juma Obayashi Kunihide Tanaka Kei Ohyama Hideki Nagae Shigeyuki Furuta Yasuji Seki Junki Koike Kevin C. Pringle Hiroaki Kitagawa |
| author_facet | Yuri Nishiya Kohei Kawaguchi Kosuke Kudo Takuya Kawaguchi Juma Obayashi Kunihide Tanaka Kei Ohyama Hideki Nagae Shigeyuki Furuta Yasuji Seki Junki Koike Kevin C. Pringle Hiroaki Kitagawa |
| author_sort | Yuri Nishiya |
| collection | DOAJ |
| description | (1) Background: Renal development involves frequent expression and loss of transcription factors, resulting in the activation of genes. Wilms’ tumor 1 (WT1), hepatocyte nuclear factor-1-beta (HNF1β), and paired box genes 2 and 8 (Pax2 and Pax8) play an important role in renal development. With this in vivo study, we examined the period and location of expression of these factors in renal development. (2) Methods: Fetal lamb kidneys (50 days from gestation to term) and adult ewe kidneys were evaluated by hematoxylin and eosin staining. Serial sections were subjected to immunohistochemistry for WT1, HNF1β, Pax2, and Pax8. (3) Results: Pax2, Pax8, and HNF1β expression was observed in the ureteric bud and collecting duct epithelial cells. We observed expression of WT1 alone in metanephric mesenchymal cells, glomerular epithelial cells, and interstitial cells in the medullary rays and Pax8 and HNF1β expression in tubular epithelial cells. WT1 was highly expressed in cells more proximal to the medulla in renal vesicles and in C- and S-shaped bodies. Pax2 was expressed in the middle and peripheral regions, and HNF1β in cells in the region in the middle of these. (4) Conclusions: WT1 is involved in nephron development. Pax2, Pax8, and HNF1β are involved in nephron maturation and the formation of peripheral collecting ducts from the Wolffian duct. |
| first_indexed | 2024-03-10T10:15:32Z |
| format | Article |
| id | doaj.art-5135fa5cb7654a2bb3cec767015b98ac |
| institution | Directory Open Access Journal |
| issn | 2221-3759 |
| language | English |
| last_indexed | 2024-03-10T10:15:32Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Developmental Biology |
| spelling | doaj.art-5135fa5cb7654a2bb3cec767015b98ac2023-11-22T00:53:03ZengMDPI AGJournal of Developmental Biology2221-37592021-06-01922210.3390/jdb9020022The Expression of Transcription Factors in Fetal Lamb KidneyYuri Nishiya0Kohei Kawaguchi1Kosuke Kudo2Takuya Kawaguchi3Juma Obayashi4Kunihide Tanaka5Kei Ohyama6Hideki Nagae7Shigeyuki Furuta8Yasuji Seki9Junki Koike10Kevin C. Pringle11Hiroaki Kitagawa12Division of Pediatric Surgery, School of Medicine, St. Marianna University, 2-16-1, Sugao, Kawasaki 216-8511, Kanagawa, JapanDivision of Pediatric Surgery, School of Medicine, St. Marianna University, 2-16-1, Sugao, Kawasaki 216-8511, Kanagawa, JapanDivision of Pediatric Surgery, School of Medicine, St. Marianna University, 2-16-1, Sugao, Kawasaki 216-8511, Kanagawa, JapanDivision of Pediatric Surgery, School of Medicine, St. Marianna University, 2-16-1, Sugao, Kawasaki 216-8511, Kanagawa, JapanDivision of Pediatric Surgery, School of Medicine, St. Marianna University, 2-16-1, Sugao, Kawasaki 216-8511, Kanagawa, JapanDivision of Pediatric Surgery, School of Medicine, St. Marianna University, 2-16-1, Sugao, Kawasaki 216-8511, Kanagawa, JapanDivision of Pediatric Surgery, School of Medicine, St. Marianna University, 2-16-1, Sugao, Kawasaki 216-8511, Kanagawa, JapanDivision of Pediatric Surgery, School of Medicine, St. Marianna University, 2-16-1, Sugao, Kawasaki 216-8511, Kanagawa, JapanDivision of Pediatric Surgery, School of Medicine, St. Marianna University, 2-16-1, Sugao, Kawasaki 216-8511, Kanagawa, JapanDivision of Pediatric Surgery, School of Medicine, St. Marianna University, 2-16-1, Sugao, Kawasaki 216-8511, Kanagawa, JapanDepartment of Pathology, School of Medicine, St. Marianna University, Kawasaki 216-8511, Kanagawa, JapanDepartment of Obstetrics and Gynecology, School of Medicine & Health Science, University of Otago, North Dunedin, Dunedin 9016, New ZealandDivision of Pediatric Surgery, School of Medicine, St. Marianna University, 2-16-1, Sugao, Kawasaki 216-8511, Kanagawa, Japan(1) Background: Renal development involves frequent expression and loss of transcription factors, resulting in the activation of genes. Wilms’ tumor 1 (WT1), hepatocyte nuclear factor-1-beta (HNF1β), and paired box genes 2 and 8 (Pax2 and Pax8) play an important role in renal development. With this in vivo study, we examined the period and location of expression of these factors in renal development. (2) Methods: Fetal lamb kidneys (50 days from gestation to term) and adult ewe kidneys were evaluated by hematoxylin and eosin staining. Serial sections were subjected to immunohistochemistry for WT1, HNF1β, Pax2, and Pax8. (3) Results: Pax2, Pax8, and HNF1β expression was observed in the ureteric bud and collecting duct epithelial cells. We observed expression of WT1 alone in metanephric mesenchymal cells, glomerular epithelial cells, and interstitial cells in the medullary rays and Pax8 and HNF1β expression in tubular epithelial cells. WT1 was highly expressed in cells more proximal to the medulla in renal vesicles and in C- and S-shaped bodies. Pax2 was expressed in the middle and peripheral regions, and HNF1β in cells in the region in the middle of these. (4) Conclusions: WT1 is involved in nephron development. Pax2, Pax8, and HNF1β are involved in nephron maturation and the formation of peripheral collecting ducts from the Wolffian duct.https://www.mdpi.com/2221-3759/9/2/22kidneydevelopmentWT1Pax2Pax8HNF1β |
| spellingShingle | Yuri Nishiya Kohei Kawaguchi Kosuke Kudo Takuya Kawaguchi Juma Obayashi Kunihide Tanaka Kei Ohyama Hideki Nagae Shigeyuki Furuta Yasuji Seki Junki Koike Kevin C. Pringle Hiroaki Kitagawa The Expression of Transcription Factors in Fetal Lamb Kidney Journal of Developmental Biology kidney development WT1 Pax2 Pax8 HNF1β |
| title | The Expression of Transcription Factors in Fetal Lamb Kidney |
| title_full | The Expression of Transcription Factors in Fetal Lamb Kidney |
| title_fullStr | The Expression of Transcription Factors in Fetal Lamb Kidney |
| title_full_unstemmed | The Expression of Transcription Factors in Fetal Lamb Kidney |
| title_short | The Expression of Transcription Factors in Fetal Lamb Kidney |
| title_sort | expression of transcription factors in fetal lamb kidney |
| topic | kidney development WT1 Pax2 Pax8 HNF1β |
| url | https://www.mdpi.com/2221-3759/9/2/22 |
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