Hypoxia-preconditioned mesenchymal stem cells attenuate peritoneal adhesion through TGF-β inhibition
Background Peritoneal adhesions (PAs) are generally described as fibrous bands between intra-abdominal organs following an abdominal surgical operation. The definitive treatments of PAs are currently ineffective yet. Hypoxia-mesenchymal stem cells (H-MSCs) have a higher capability to survive at the...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine Trisakti University
2020-07-01
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| Series: | Universa Medicina |
| Subjects: | |
| Online Access: | https://univmed.org/ejurnal/index.php/medicina/article/view/902 |
| _version_ | 1818901114091732992 |
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| author | Setyo Trisnadi Adi Muradi Muhar Agung Putra Azizah Retno Kustiyah |
| author_facet | Setyo Trisnadi Adi Muradi Muhar Agung Putra Azizah Retno Kustiyah |
| author_sort | Setyo Trisnadi |
| collection | DOAJ |
| description | Background
Peritoneal adhesions (PAs) are generally described as fibrous bands between intra-abdominal organs following an abdominal surgical operation. The definitive treatments of PAs are currently ineffective yet. Hypoxia-mesenchymal stem cells (H-MSCs) have a higher capability to survive at the site of injury than normoxia-MSCs (N-MSCs) to repair injured tissue without fibrosis. This study aimed to analyze the effect of H-MSCs in controlling formation of PAs by reducing TGF-β level in a rat model.
Methods
A study of post-test only control group design was conducted, involving eighteen PA rat models weighing 250 ± 25 g that were randomly assigned into 3 groups, comprising control group (C), and groups T1 and T2 receiving H-MSC treatment at doses of 3 x 106 and 1.5 x 106, respectively. To induce H-MSCs, MSCs were incubated in hypoxic conditions at 5% O2 and 37oC for 24 hours. Expression level of TGF-β was analyzed by enzyme-linked immunosorbent assay (ELISA) at 450 nm and adhesion formation was described macroscopically. The Kruskal-Wallis variance analysis was used to analyze significant differences among the groups.
Results
The results of this study showed that H-MSCs in group T1 inhibited TGF-β expression significantly on day 8 (p<0.001) and day 14 (p<0.05). Moreover, there was almost no adhesion apparent following H-MSC administration in group T1.
Conclusions
Based on this study, we conclude that H-MSCs may attenuate PA formation following inhibition of TGF-β expression in the PA rat model. |
| first_indexed | 2024-12-19T20:14:36Z |
| format | Article |
| id | doaj.art-513a838719fe45a9b4d03e3edc21eb39 |
| institution | Directory Open Access Journal |
| issn | 1907-3062 2407-2230 |
| language | English |
| last_indexed | 2024-12-19T20:14:36Z |
| publishDate | 2020-07-01 |
| publisher | Faculty of Medicine Trisakti University |
| record_format | Article |
| series | Universa Medicina |
| spelling | doaj.art-513a838719fe45a9b4d03e3edc21eb392022-12-21T20:07:13ZengFaculty of Medicine Trisakti UniversityUniversa Medicina1907-30622407-22302020-07-0139210.18051/UnivMed.2020.v39.97-104406Hypoxia-preconditioned mesenchymal stem cells attenuate peritoneal adhesion through TGF-β inhibitionSetyo Trisnadi0Adi Muradi Muhar1Agung Putra2Azizah Retno Kustiyah3Department of Forensic Medicine, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central JavaDepartment of Surgical, Medical Faculty, Universitas Sumatera Utara (USU), Semarang, Central JavaStem Cell and Cancer Research, Sultan Agung Islamic University (UNISSULA), Semarang, Central JavaDepartment of Pediatric, Medical Faculty, Sultan Agung Islamic University (UNISSULA), Semarang, Central JavaBackground Peritoneal adhesions (PAs) are generally described as fibrous bands between intra-abdominal organs following an abdominal surgical operation. The definitive treatments of PAs are currently ineffective yet. Hypoxia-mesenchymal stem cells (H-MSCs) have a higher capability to survive at the site of injury than normoxia-MSCs (N-MSCs) to repair injured tissue without fibrosis. This study aimed to analyze the effect of H-MSCs in controlling formation of PAs by reducing TGF-β level in a rat model. Methods A study of post-test only control group design was conducted, involving eighteen PA rat models weighing 250 ± 25 g that were randomly assigned into 3 groups, comprising control group (C), and groups T1 and T2 receiving H-MSC treatment at doses of 3 x 106 and 1.5 x 106, respectively. To induce H-MSCs, MSCs were incubated in hypoxic conditions at 5% O2 and 37oC for 24 hours. Expression level of TGF-β was analyzed by enzyme-linked immunosorbent assay (ELISA) at 450 nm and adhesion formation was described macroscopically. The Kruskal-Wallis variance analysis was used to analyze significant differences among the groups. Results The results of this study showed that H-MSCs in group T1 inhibited TGF-β expression significantly on day 8 (p<0.001) and day 14 (p<0.05). Moreover, there was almost no adhesion apparent following H-MSC administration in group T1. Conclusions Based on this study, we conclude that H-MSCs may attenuate PA formation following inhibition of TGF-β expression in the PA rat model.https://univmed.org/ejurnal/index.php/medicina/article/view/902Peritoneal adhesionshypoxiamesenchymal stem cellsTGF-β |
| spellingShingle | Setyo Trisnadi Adi Muradi Muhar Agung Putra Azizah Retno Kustiyah Hypoxia-preconditioned mesenchymal stem cells attenuate peritoneal adhesion through TGF-β inhibition Universa Medicina Peritoneal adhesions hypoxia mesenchymal stem cells TGF-β |
| title | Hypoxia-preconditioned mesenchymal stem cells attenuate peritoneal adhesion through TGF-β inhibition |
| title_full | Hypoxia-preconditioned mesenchymal stem cells attenuate peritoneal adhesion through TGF-β inhibition |
| title_fullStr | Hypoxia-preconditioned mesenchymal stem cells attenuate peritoneal adhesion through TGF-β inhibition |
| title_full_unstemmed | Hypoxia-preconditioned mesenchymal stem cells attenuate peritoneal adhesion through TGF-β inhibition |
| title_short | Hypoxia-preconditioned mesenchymal stem cells attenuate peritoneal adhesion through TGF-β inhibition |
| title_sort | hypoxia preconditioned mesenchymal stem cells attenuate peritoneal adhesion through tgf β inhibition |
| topic | Peritoneal adhesions hypoxia mesenchymal stem cells TGF-β |
| url | https://univmed.org/ejurnal/index.php/medicina/article/view/902 |
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