Genome-wide transcriptome profiling of transgenic hop (Humulus lupulus L.) constitutively overexpressing HlWRKY1 and HlWDR1 transcription factors
Abstract Background The hop plant (Humulus lupulus L.) is a valuable source of several secondary metabolites, such as flavonoids, bitter acids, and essential oils. These compounds are widely implicated in the beer brewing industry and are having potential biomedical applications. Several independent...
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| Language: | English |
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BMC
2018-10-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-018-5125-8 |
| _version_ | 1819056310467952640 |
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| author | Ajay Kumar Mishra Ganesh Selvaraj Duraisamy Mudra Khare Tomáš Kocábek Jernej Jakse Jindřich Bříza Josef Patzak Teruo Sano Jaroslav Matoušek |
| author_facet | Ajay Kumar Mishra Ganesh Selvaraj Duraisamy Mudra Khare Tomáš Kocábek Jernej Jakse Jindřich Bříza Josef Patzak Teruo Sano Jaroslav Matoušek |
| author_sort | Ajay Kumar Mishra |
| collection | DOAJ |
| description | Abstract Background The hop plant (Humulus lupulus L.) is a valuable source of several secondary metabolites, such as flavonoids, bitter acids, and essential oils. These compounds are widely implicated in the beer brewing industry and are having potential biomedical applications. Several independent breeding programs around the world have been initiated to develop new cultivars with enriched lupulin and secondary metabolite contents but met with limited success due to several constraints. In the present work, a pioneering attempt has been made to overexpress master regulator binary transcription factor complex formed by HlWRKY1 and HlWDR1 using a plant expression vector to enhance the level of prenylflavonoid and bitter acid content in the hop. Subsequently, we performed transcriptional profiling using high-throughput RNA-Seq technology in leaves of resultant transformants and wild-type hop to gain in-depth information about the genome-wide functional changes induced by HlWRKY1 and HlWDR1 overexpression. Results The transgenic WW-lines exhibited an elevated expression of structural and regulatory genes involved in prenylflavonoid and bitter acid biosynthesis pathways. In addition, the comparative transcriptome analysis revealed a total of 522 transcripts involved in 30 pathways, including lipids and amino acids biosynthesis, primary carbon metabolism, phytohormone signaling and stress responses were differentially expressed in WW-transformants. It was apparent from the whole transcriptome sequencing that modulation of primary carbon metabolism and other pathways by HlWRKY1 and HlWDR1 overexpression resulted in enhanced substrate flux towards secondary metabolites pathway. The detailed analyses suggested that none of the pathways or genes, which have a detrimental effect on physiology, growth and development processes, were induced on a genome-wide scale in WW-transgenic lines. Conclusions Taken together, our results suggest that HlWRKY1 and HlWDR1 simultaneous overexpression positively regulates the prenylflavonoid and bitter acid biosynthesis pathways in the hop and thus these transgenes are presented as prospective candidates for achieving enhanced secondary metabolite content in the hop. |
| first_indexed | 2024-12-21T13:21:23Z |
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| id | doaj.art-513f6131abe7425eb2b33dcc577fe44f |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-21T13:21:23Z |
| publishDate | 2018-10-01 |
| publisher | BMC |
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| series | BMC Genomics |
| spelling | doaj.art-513f6131abe7425eb2b33dcc577fe44f2022-12-21T19:02:34ZengBMCBMC Genomics1471-21642018-10-0119111810.1186/s12864-018-5125-8Genome-wide transcriptome profiling of transgenic hop (Humulus lupulus L.) constitutively overexpressing HlWRKY1 and HlWDR1 transcription factorsAjay Kumar Mishra0Ganesh Selvaraj Duraisamy1Mudra Khare2Tomáš Kocábek3Jernej Jakse4Jindřich Bříza5Josef Patzak6Teruo Sano7Jaroslav Matoušek8Biology Centre of the Czech Academy of Sciences, Institute of Plant Molecular Biology, Department of Molecular GeneticsBiology Centre of the Czech Academy of Sciences, Institute of Plant Molecular Biology, Department of Molecular GeneticsBiology Centre of the Czech Academy of Sciences, Institute of Plant Molecular Biology, Department of Molecular GeneticsBiology Centre of the Czech Academy of Sciences, Institute of Plant Molecular Biology, Department of Molecular GeneticsDepartment of Agronomy, Biotechnical Faculty, University of LjubljanaBiology Centre of the Czech Academy of Sciences, Institute of Plant Molecular Biology, Department of Molecular GeneticsHop Research Institute, Co. Ltd.Faculty of Agriculture and Life Science, Department of Applied Biosciences, Hirosaki UniversityBiology Centre of the Czech Academy of Sciences, Institute of Plant Molecular Biology, Department of Molecular GeneticsAbstract Background The hop plant (Humulus lupulus L.) is a valuable source of several secondary metabolites, such as flavonoids, bitter acids, and essential oils. These compounds are widely implicated in the beer brewing industry and are having potential biomedical applications. Several independent breeding programs around the world have been initiated to develop new cultivars with enriched lupulin and secondary metabolite contents but met with limited success due to several constraints. In the present work, a pioneering attempt has been made to overexpress master regulator binary transcription factor complex formed by HlWRKY1 and HlWDR1 using a plant expression vector to enhance the level of prenylflavonoid and bitter acid content in the hop. Subsequently, we performed transcriptional profiling using high-throughput RNA-Seq technology in leaves of resultant transformants and wild-type hop to gain in-depth information about the genome-wide functional changes induced by HlWRKY1 and HlWDR1 overexpression. Results The transgenic WW-lines exhibited an elevated expression of structural and regulatory genes involved in prenylflavonoid and bitter acid biosynthesis pathways. In addition, the comparative transcriptome analysis revealed a total of 522 transcripts involved in 30 pathways, including lipids and amino acids biosynthesis, primary carbon metabolism, phytohormone signaling and stress responses were differentially expressed in WW-transformants. It was apparent from the whole transcriptome sequencing that modulation of primary carbon metabolism and other pathways by HlWRKY1 and HlWDR1 overexpression resulted in enhanced substrate flux towards secondary metabolites pathway. The detailed analyses suggested that none of the pathways or genes, which have a detrimental effect on physiology, growth and development processes, were induced on a genome-wide scale in WW-transgenic lines. Conclusions Taken together, our results suggest that HlWRKY1 and HlWDR1 simultaneous overexpression positively regulates the prenylflavonoid and bitter acid biosynthesis pathways in the hop and thus these transgenes are presented as prospective candidates for achieving enhanced secondary metabolite content in the hop.http://link.springer.com/article/10.1186/s12864-018-5125-8Bitter acidsFlavonoidsGenetic transformationHumulus lupulusSecondary metaboliteTranscription factors |
| spellingShingle | Ajay Kumar Mishra Ganesh Selvaraj Duraisamy Mudra Khare Tomáš Kocábek Jernej Jakse Jindřich Bříza Josef Patzak Teruo Sano Jaroslav Matoušek Genome-wide transcriptome profiling of transgenic hop (Humulus lupulus L.) constitutively overexpressing HlWRKY1 and HlWDR1 transcription factors BMC Genomics Bitter acids Flavonoids Genetic transformation Humulus lupulus Secondary metabolite Transcription factors |
| title | Genome-wide transcriptome profiling of transgenic hop (Humulus lupulus L.) constitutively overexpressing HlWRKY1 and HlWDR1 transcription factors |
| title_full | Genome-wide transcriptome profiling of transgenic hop (Humulus lupulus L.) constitutively overexpressing HlWRKY1 and HlWDR1 transcription factors |
| title_fullStr | Genome-wide transcriptome profiling of transgenic hop (Humulus lupulus L.) constitutively overexpressing HlWRKY1 and HlWDR1 transcription factors |
| title_full_unstemmed | Genome-wide transcriptome profiling of transgenic hop (Humulus lupulus L.) constitutively overexpressing HlWRKY1 and HlWDR1 transcription factors |
| title_short | Genome-wide transcriptome profiling of transgenic hop (Humulus lupulus L.) constitutively overexpressing HlWRKY1 and HlWDR1 transcription factors |
| title_sort | genome wide transcriptome profiling of transgenic hop humulus lupulus l constitutively overexpressing hlwrky1 and hlwdr1 transcription factors |
| topic | Bitter acids Flavonoids Genetic transformation Humulus lupulus Secondary metabolite Transcription factors |
| url | http://link.springer.com/article/10.1186/s12864-018-5125-8 |
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