Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang
Abstract Background Sanghuangporus sanghuang is a well-known traditional medicinal mushroom associated with mulberry. Despite the properties of this mushroom being known for many years, the regulatory mechanisms of bioactive compound biosynthesis in this medicinal mushroom are still unclear. Lysine...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-11-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-08120-0 |
| _version_ | 1819198405365202944 |
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| author | Tong Wang Guangyuan Wang Guoli Zhang Ranran Hou Liwei Zhou Xuemei Tian |
| author_facet | Tong Wang Guangyuan Wang Guoli Zhang Ranran Hou Liwei Zhou Xuemei Tian |
| author_sort | Tong Wang |
| collection | DOAJ |
| description | Abstract Background Sanghuangporus sanghuang is a well-known traditional medicinal mushroom associated with mulberry. Despite the properties of this mushroom being known for many years, the regulatory mechanisms of bioactive compound biosynthesis in this medicinal mushroom are still unclear. Lysine malonylation is a posttranslational modification that has many critical functions in various aspects of cell metabolism. However, at present we do not know its role in S. sanghuang. In this study, a global investigation of the lysine malonylome in S. sanghuang was therefore carried out. Results In total, 714 malonyl modification sites were matched to 255 different proteins. The analysis indicated that malonyl modifications were involved in a wide range of cellular functions and displayed a distinct subcellular localization. Bioinformatics analysis indicated that malonylated proteins were engaged in different metabolic pathways, including glyoxylate and dicarboxylate metabolism, glycolysis/gluconeogenesis, and the tricarboxylic acid (TCA) cycle. Notably, a total of 26 enzymes related to triterpene and polysaccharide biosynthesis were found to be malonylated, indicating an indispensable role of lysine malonylation in bioactive compound biosynthesis in S. sanghuang. Conclusions These findings suggest that malonylation is associated with many metabolic pathways, particularly the metabolism of the bioactive compounds triterpene and polysaccharide. This paper represents the first comprehensive survey of malonylation in S. sanghuang and provides important data for further study on the physiological function of lysine malonylation in S. sanghuang and other medicinal mushrooms. |
| first_indexed | 2024-12-23T02:59:55Z |
| format | Article |
| id | doaj.art-513f8084b27747938b234b4de6f4fadd |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-23T02:59:55Z |
| publishDate | 2021-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-513f8084b27747938b234b4de6f4fadd2022-12-21T18:02:27ZengBMCBMC Genomics1471-21642021-11-0122111110.1186/s12864-021-08120-0Systematic analysis of the lysine malonylome in Sanghuangporus sanghuangTong Wang0Guangyuan Wang1Guoli Zhang2Ranran Hou3Liwei Zhou4Xuemei Tian5Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural UniversityShandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural UniversityShandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural UniversityShandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural UniversityState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of SciencesShandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural UniversityAbstract Background Sanghuangporus sanghuang is a well-known traditional medicinal mushroom associated with mulberry. Despite the properties of this mushroom being known for many years, the regulatory mechanisms of bioactive compound biosynthesis in this medicinal mushroom are still unclear. Lysine malonylation is a posttranslational modification that has many critical functions in various aspects of cell metabolism. However, at present we do not know its role in S. sanghuang. In this study, a global investigation of the lysine malonylome in S. sanghuang was therefore carried out. Results In total, 714 malonyl modification sites were matched to 255 different proteins. The analysis indicated that malonyl modifications were involved in a wide range of cellular functions and displayed a distinct subcellular localization. Bioinformatics analysis indicated that malonylated proteins were engaged in different metabolic pathways, including glyoxylate and dicarboxylate metabolism, glycolysis/gluconeogenesis, and the tricarboxylic acid (TCA) cycle. Notably, a total of 26 enzymes related to triterpene and polysaccharide biosynthesis were found to be malonylated, indicating an indispensable role of lysine malonylation in bioactive compound biosynthesis in S. sanghuang. Conclusions These findings suggest that malonylation is associated with many metabolic pathways, particularly the metabolism of the bioactive compounds triterpene and polysaccharide. This paper represents the first comprehensive survey of malonylation in S. sanghuang and provides important data for further study on the physiological function of lysine malonylation in S. sanghuang and other medicinal mushrooms.https://doi.org/10.1186/s12864-021-08120-0MalonylationPosttranslational modification (PTM)MalonylproteomeSanghuangporus sanghuang |
| spellingShingle | Tong Wang Guangyuan Wang Guoli Zhang Ranran Hou Liwei Zhou Xuemei Tian Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang BMC Genomics Malonylation Posttranslational modification (PTM) Malonylproteome Sanghuangporus sanghuang |
| title | Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang |
| title_full | Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang |
| title_fullStr | Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang |
| title_full_unstemmed | Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang |
| title_short | Systematic analysis of the lysine malonylome in Sanghuangporus sanghuang |
| title_sort | systematic analysis of the lysine malonylome in sanghuangporus sanghuang |
| topic | Malonylation Posttranslational modification (PTM) Malonylproteome Sanghuangporus sanghuang |
| url | https://doi.org/10.1186/s12864-021-08120-0 |
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