Longitudinal bioluminescence imaging to monitor breast tumor growth and treatment response using the chick chorioallantoic membrane model
Abstract The development of successful treatment regimens for breast cancer requires strong pre-clinical data generated in physiologically relevant pre-clinical models. Here we report the development of the chick embryo chorioallantoic membrane (CAM) model to study tumor growth and angiogenesis usin...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-10-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-20854-9 |
| _version_ | 1811182273082425344 |
|---|---|
| author | Sumreen Javed Sepideh Soukhtehzari Nazarine Fernandes Karla C. Williams |
| author_facet | Sumreen Javed Sepideh Soukhtehzari Nazarine Fernandes Karla C. Williams |
| author_sort | Sumreen Javed |
| collection | DOAJ |
| description | Abstract The development of successful treatment regimens for breast cancer requires strong pre-clinical data generated in physiologically relevant pre-clinical models. Here we report the development of the chick embryo chorioallantoic membrane (CAM) model to study tumor growth and angiogenesis using breast cancer cell lines. MDA-MB-231 and MCF7 tumor cell lines were engrafted onto the chick embryo CAM to study tumor growth and treatment response. Tumor growth was evaluated through bioluminescence imaging and a significant increase in tumor size and vascularization was found over a 9-day period. We then evaluated the impact of anti-angiogenic drugs, axitinib and bevacizumab, on tumor growth and angiogenesis. Drug treatment significantly reduced tumor vascularization and size. Overall, our findings demonstrate that the chick embryo CAM is a clinically relevant model to monitor therapeutic response in breast cancer and can be used as a platform for drug screening to evaluate not only gross changes in tumor burden but physiological processes such as angiogenesis. |
| first_indexed | 2024-04-11T09:30:04Z |
| format | Article |
| id | doaj.art-5143e5409bbd495eb8b697efc3164fe1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T09:30:04Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5143e5409bbd495eb8b697efc3164fe12022-12-22T04:31:55ZengNature PortfolioScientific Reports2045-23222022-10-0112111010.1038/s41598-022-20854-9Longitudinal bioluminescence imaging to monitor breast tumor growth and treatment response using the chick chorioallantoic membrane modelSumreen Javed0Sepideh Soukhtehzari1Nazarine Fernandes2Karla C. Williams3Faculty of Pharmaceutical Sciences, The University of British ColumbiaFaculty of Pharmaceutical Sciences, The University of British ColumbiaFaculty of Pharmaceutical Sciences, The University of British ColumbiaFaculty of Pharmaceutical Sciences, The University of British ColumbiaAbstract The development of successful treatment regimens for breast cancer requires strong pre-clinical data generated in physiologically relevant pre-clinical models. Here we report the development of the chick embryo chorioallantoic membrane (CAM) model to study tumor growth and angiogenesis using breast cancer cell lines. MDA-MB-231 and MCF7 tumor cell lines were engrafted onto the chick embryo CAM to study tumor growth and treatment response. Tumor growth was evaluated through bioluminescence imaging and a significant increase in tumor size and vascularization was found over a 9-day period. We then evaluated the impact of anti-angiogenic drugs, axitinib and bevacizumab, on tumor growth and angiogenesis. Drug treatment significantly reduced tumor vascularization and size. Overall, our findings demonstrate that the chick embryo CAM is a clinically relevant model to monitor therapeutic response in breast cancer and can be used as a platform for drug screening to evaluate not only gross changes in tumor burden but physiological processes such as angiogenesis.https://doi.org/10.1038/s41598-022-20854-9 |
| spellingShingle | Sumreen Javed Sepideh Soukhtehzari Nazarine Fernandes Karla C. Williams Longitudinal bioluminescence imaging to monitor breast tumor growth and treatment response using the chick chorioallantoic membrane model Scientific Reports |
| title | Longitudinal bioluminescence imaging to monitor breast tumor growth and treatment response using the chick chorioallantoic membrane model |
| title_full | Longitudinal bioluminescence imaging to monitor breast tumor growth and treatment response using the chick chorioallantoic membrane model |
| title_fullStr | Longitudinal bioluminescence imaging to monitor breast tumor growth and treatment response using the chick chorioallantoic membrane model |
| title_full_unstemmed | Longitudinal bioluminescence imaging to monitor breast tumor growth and treatment response using the chick chorioallantoic membrane model |
| title_short | Longitudinal bioluminescence imaging to monitor breast tumor growth and treatment response using the chick chorioallantoic membrane model |
| title_sort | longitudinal bioluminescence imaging to monitor breast tumor growth and treatment response using the chick chorioallantoic membrane model |
| url | https://doi.org/10.1038/s41598-022-20854-9 |
| work_keys_str_mv | AT sumreenjaved longitudinalbioluminescenceimagingtomonitorbreasttumorgrowthandtreatmentresponseusingthechickchorioallantoicmembranemodel AT sepidehsoukhtehzari longitudinalbioluminescenceimagingtomonitorbreasttumorgrowthandtreatmentresponseusingthechickchorioallantoicmembranemodel AT nazarinefernandes longitudinalbioluminescenceimagingtomonitorbreasttumorgrowthandtreatmentresponseusingthechickchorioallantoicmembranemodel AT karlacwilliams longitudinalbioluminescenceimagingtomonitorbreasttumorgrowthandtreatmentresponseusingthechickchorioallantoicmembranemodel |