Phenolic compounds and in vitro antioxidant activity of Moringa stenopetala grown in South Ethiopia
Moringa stenopetala is a traditional medicinal and nutritional plant in Ethiopia. The aim of this work was to carry out a chemical analysis focusing on secondary metabolites, particularly phenolic compounds, and antioxidant activity of aqueous and methanol extracts of Moringa stenopetala leaf. The p...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | International Journal of Food Properties |
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| Online Access: | http://dx.doi.org/10.1080/10942912.2021.1990943 |
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| author | Engeda Dessalegn H. P. Vasantha Rupasinghe |
| author_facet | Engeda Dessalegn H. P. Vasantha Rupasinghe |
| author_sort | Engeda Dessalegn |
| collection | DOAJ |
| description | Moringa stenopetala is a traditional medicinal and nutritional plant in Ethiopia. The aim of this work was to carry out a chemical analysis focusing on secondary metabolites, particularly phenolic compounds, and antioxidant activity of aqueous and methanol extracts of Moringa stenopetala leaf. The phenolic compounds were analyzed by high-performance liquid chromatography diode array detector (HPLC-DAD). Hydroxycinnamic acid (538 ± 6 µg/g), 3-Hydroxbenzoic acid (31 ± 6 µg/g), and quercetin-3-O-rutinoside (1155 ± 65 µg/g) were the major components in methanol extract. Whereas, syringic (84 ± 13 µg/g), chlorogenic (165 ± 19 µg/g), succinnic (1811 ± 105 µg/g), and fumaric (1582 ± 65 µg/g) acids were the major organic acids in the aqueous extract. Methanol extract had higher total flavonoid (11 ± 2 mg of catechin equivalent per gram of dried extract) and total phenolic (39 ± 3 mg of gallic acid equivalent per gram of dried extract) contents. This extract showed stronger DPPH scavenging (EC50 = 78 ± 6 μg/mL) and ferrous chelating (EC50 = 239 ± 12 μg/mL) activities. Due to the abundance sources of bioactive compounds and antioxidant activity, the dried leaf of M. stenopetala could be used for the development of nutraceuticals or incorporated into functional foods. |
| first_indexed | 2024-04-11T17:33:18Z |
| format | Article |
| id | doaj.art-51451c3aab824d7399bef91b1ce9108b |
| institution | Directory Open Access Journal |
| issn | 1094-2912 1532-2386 |
| language | English |
| last_indexed | 2024-04-11T17:33:18Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Food Properties |
| spelling | doaj.art-51451c3aab824d7399bef91b1ce9108b2022-12-22T04:11:57ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862021-01-012411681169210.1080/10942912.2021.19909431990943Phenolic compounds and in vitro antioxidant activity of Moringa stenopetala grown in South EthiopiaEngeda Dessalegn0H. P. Vasantha Rupasinghe1Hawassa College of EducationDalhousie UniversityMoringa stenopetala is a traditional medicinal and nutritional plant in Ethiopia. The aim of this work was to carry out a chemical analysis focusing on secondary metabolites, particularly phenolic compounds, and antioxidant activity of aqueous and methanol extracts of Moringa stenopetala leaf. The phenolic compounds were analyzed by high-performance liquid chromatography diode array detector (HPLC-DAD). Hydroxycinnamic acid (538 ± 6 µg/g), 3-Hydroxbenzoic acid (31 ± 6 µg/g), and quercetin-3-O-rutinoside (1155 ± 65 µg/g) were the major components in methanol extract. Whereas, syringic (84 ± 13 µg/g), chlorogenic (165 ± 19 µg/g), succinnic (1811 ± 105 µg/g), and fumaric (1582 ± 65 µg/g) acids were the major organic acids in the aqueous extract. Methanol extract had higher total flavonoid (11 ± 2 mg of catechin equivalent per gram of dried extract) and total phenolic (39 ± 3 mg of gallic acid equivalent per gram of dried extract) contents. This extract showed stronger DPPH scavenging (EC50 = 78 ± 6 μg/mL) and ferrous chelating (EC50 = 239 ± 12 μg/mL) activities. Due to the abundance sources of bioactive compounds and antioxidant activity, the dried leaf of M. stenopetala could be used for the development of nutraceuticals or incorporated into functional foods.http://dx.doi.org/10.1080/10942912.2021.1990943antioxidantchelatinghplc-dadmoringaphenolic compounds |
| spellingShingle | Engeda Dessalegn H. P. Vasantha Rupasinghe Phenolic compounds and in vitro antioxidant activity of Moringa stenopetala grown in South Ethiopia International Journal of Food Properties antioxidant chelating hplc-dad moringa phenolic compounds |
| title | Phenolic compounds and in vitro antioxidant activity of Moringa stenopetala grown in South Ethiopia |
| title_full | Phenolic compounds and in vitro antioxidant activity of Moringa stenopetala grown in South Ethiopia |
| title_fullStr | Phenolic compounds and in vitro antioxidant activity of Moringa stenopetala grown in South Ethiopia |
| title_full_unstemmed | Phenolic compounds and in vitro antioxidant activity of Moringa stenopetala grown in South Ethiopia |
| title_short | Phenolic compounds and in vitro antioxidant activity of Moringa stenopetala grown in South Ethiopia |
| title_sort | phenolic compounds and in vitro antioxidant activity of moringa stenopetala grown in south ethiopia |
| topic | antioxidant chelating hplc-dad moringa phenolic compounds |
| url | http://dx.doi.org/10.1080/10942912.2021.1990943 |
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