DK-MGAR101, an extract of adventitious roots of mountain ginseng, improves blood circulation by inhibiting endothelial cell injury, platelet aggregation, and thrombus formation
Background: Since ginsenosides exert an anti-thrombotic activity, blood flow-improving effects of DK-MGAR101, an extract of mountain ginseng adventitious roots (MGAR) containing various ginsenosides, were investigated in comparison with an extract of Korean Red Ginseng (ERG). Methods: In Sprague-Daw...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S122684532200001X |
| _version_ | 1811281344070680576 |
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| author | Hye Rim Seong Cuicui Wang Muhammad Irfan Young Eun Kim Gooyoung Jung Sung Kyeong Park Tae Myoung Kim Ehn-Kyoung Choi Man Hee Rhee Yun-Bae Kim |
| author_facet | Hye Rim Seong Cuicui Wang Muhammad Irfan Young Eun Kim Gooyoung Jung Sung Kyeong Park Tae Myoung Kim Ehn-Kyoung Choi Man Hee Rhee Yun-Bae Kim |
| author_sort | Hye Rim Seong |
| collection | DOAJ |
| description | Background: Since ginsenosides exert an anti-thrombotic activity, blood flow-improving effects of DK-MGAR101, an extract of mountain ginseng adventitious roots (MGAR) containing various ginsenosides, were investigated in comparison with an extract of Korean Red Ginseng (ERG). Methods: In Sprague-Dawley rats orally administered with DK-MGAR101 or ERG, oxidative carotid arterial thrombosis was induced with FeCl3 (35%), and their blood flow and occlusion time were measured. To elucidate underlying mechanisms, the cytoprotective activities on rat aortic endothelial cells (RAOECs) exposed to hydrogen peroxide (H2O2) were confirmed. In addition, the inhibitory activities of DK-MGAR101 and ERG on agonist-induced platelet aggregation, thromboxane B2 production, and ATP granule release from stimulated platelets as well as blood coagulation were analyzed. Results: DK-MGAR101 containing high concentrations of Rb1, Rg1, Rg3, Rg5, and Rk1 ginsenosides (55.07 mg/g) was more effective than ERG (ginsenosides 8.45 mg/g) in protecting RAOECs against H2O2 cytotoxicity. DK-MGAR101 was superior to ERG not only in suppressing platelet aggregation, thromboxane B2 production, and granule release, but also in delaying blood coagulation, FeCl3-induced arterial occlusion, and thrombus formation. Conclusions: The results indicate that DK-MGAR101 prevents blood vessel occlusion by suppressing platelet aggregation, thrombosis, and blood coagulation, in addition to endothelial cell injury. |
| first_indexed | 2024-04-13T01:31:58Z |
| format | Article |
| id | doaj.art-514608482d3a4f9684355252214adf5d |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-04-13T01:31:58Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-514608482d3a4f9684355252214adf5d2022-12-22T03:08:29ZengElsevierJournal of Ginseng Research1226-84532022-09-01465683689DK-MGAR101, an extract of adventitious roots of mountain ginseng, improves blood circulation by inhibiting endothelial cell injury, platelet aggregation, and thrombus formationHye Rim Seong0Cuicui Wang1Muhammad Irfan2Young Eun Kim3Gooyoung Jung4Sung Kyeong Park5Tae Myoung Kim6Ehn-Kyoung Choi7Man Hee Rhee8Yun-Bae Kim9College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu, Republic of KoreaPharmaceutical Technology Institute, Dongkook Pharmaceutical Co., Ltd., Jincheon, Republic of KoreaPharmaceutical Technology Institute, Dongkook Pharmaceutical Co., Ltd., Jincheon, Republic of KoreaDepartment of Beauty Care, Daejeon Health Institute of Technology, Daejeon, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea; Corresponding author. College of Veterinary Medicine, Chungbuk National University, 1 Chungdaero Gaesin-dong, Cheongju, Chungbuk, 28644, Republic of Korea.Background: Since ginsenosides exert an anti-thrombotic activity, blood flow-improving effects of DK-MGAR101, an extract of mountain ginseng adventitious roots (MGAR) containing various ginsenosides, were investigated in comparison with an extract of Korean Red Ginseng (ERG). Methods: In Sprague-Dawley rats orally administered with DK-MGAR101 or ERG, oxidative carotid arterial thrombosis was induced with FeCl3 (35%), and their blood flow and occlusion time were measured. To elucidate underlying mechanisms, the cytoprotective activities on rat aortic endothelial cells (RAOECs) exposed to hydrogen peroxide (H2O2) were confirmed. In addition, the inhibitory activities of DK-MGAR101 and ERG on agonist-induced platelet aggregation, thromboxane B2 production, and ATP granule release from stimulated platelets as well as blood coagulation were analyzed. Results: DK-MGAR101 containing high concentrations of Rb1, Rg1, Rg3, Rg5, and Rk1 ginsenosides (55.07 mg/g) was more effective than ERG (ginsenosides 8.45 mg/g) in protecting RAOECs against H2O2 cytotoxicity. DK-MGAR101 was superior to ERG not only in suppressing platelet aggregation, thromboxane B2 production, and granule release, but also in delaying blood coagulation, FeCl3-induced arterial occlusion, and thrombus formation. Conclusions: The results indicate that DK-MGAR101 prevents blood vessel occlusion by suppressing platelet aggregation, thrombosis, and blood coagulation, in addition to endothelial cell injury.http://www.sciencedirect.com/science/article/pii/S122684532200001XAdventitious rootBlood circulationGinsenosideMountain ginsengPlatelet aggregation |
| spellingShingle | Hye Rim Seong Cuicui Wang Muhammad Irfan Young Eun Kim Gooyoung Jung Sung Kyeong Park Tae Myoung Kim Ehn-Kyoung Choi Man Hee Rhee Yun-Bae Kim DK-MGAR101, an extract of adventitious roots of mountain ginseng, improves blood circulation by inhibiting endothelial cell injury, platelet aggregation, and thrombus formation Journal of Ginseng Research Adventitious root Blood circulation Ginsenoside Mountain ginseng Platelet aggregation |
| title | DK-MGAR101, an extract of adventitious roots of mountain ginseng, improves blood circulation by inhibiting endothelial cell injury, platelet aggregation, and thrombus formation |
| title_full | DK-MGAR101, an extract of adventitious roots of mountain ginseng, improves blood circulation by inhibiting endothelial cell injury, platelet aggregation, and thrombus formation |
| title_fullStr | DK-MGAR101, an extract of adventitious roots of mountain ginseng, improves blood circulation by inhibiting endothelial cell injury, platelet aggregation, and thrombus formation |
| title_full_unstemmed | DK-MGAR101, an extract of adventitious roots of mountain ginseng, improves blood circulation by inhibiting endothelial cell injury, platelet aggregation, and thrombus formation |
| title_short | DK-MGAR101, an extract of adventitious roots of mountain ginseng, improves blood circulation by inhibiting endothelial cell injury, platelet aggregation, and thrombus formation |
| title_sort | dk mgar101 an extract of adventitious roots of mountain ginseng improves blood circulation by inhibiting endothelial cell injury platelet aggregation and thrombus formation |
| topic | Adventitious root Blood circulation Ginsenoside Mountain ginseng Platelet aggregation |
| url | http://www.sciencedirect.com/science/article/pii/S122684532200001X |
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