Association of Early Atherosclerosis with Vascular Wall Shear Stress in Hypercholesterolemic Zebrafish.
Although atherosclerosis is a multifactorial disease, the role of hemodynamic information has become more important. Low and oscillating wall shear stress (WSS) that changes its direction is associated with the early stage of atherosclerosis. Several in vitro and in vivo models were proposed to reve...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4643039?pdf=render |
| _version_ | 1818924930634350592 |
|---|---|
| author | Sang Joon Lee Woorak Choi Eunseok Seo Eunseop Yeom |
| author_facet | Sang Joon Lee Woorak Choi Eunseok Seo Eunseop Yeom |
| author_sort | Sang Joon Lee |
| collection | DOAJ |
| description | Although atherosclerosis is a multifactorial disease, the role of hemodynamic information has become more important. Low and oscillating wall shear stress (WSS) that changes its direction is associated with the early stage of atherosclerosis. Several in vitro and in vivo models were proposed to reveal the relation between the WSS and the early atherosclerosis. However, these models possess technical limitations in mimicking real physiological conditions and monitoring the developmental course of the early atherosclerosis. In this study, a hypercholesterolaemic zebrafish model is proposed as a novel experimental model to resolve these limitations. Zebrafish larvae are optically transparent, which enables temporal observation of pathological variations under in vivo condition. WSS in blood vessels of 15 days post-fertilisation zebrafish was measured using a micro particle image velocimetry (PIV) technique, and spatial distribution of lipid deposition inside the model was quantitatively investigated after feeding high cholesterol diet for 10 days. Lipids were mainly deposited in blood vessel of low WSS. The oscillating WSS was not induced by the blood flows in zebrafish models. The present hypercholesterolaemic zebrafish would be used as a potentially useful model for in vivo study about the effects of low WSS in the early atherosclerosis. |
| first_indexed | 2024-12-20T02:33:09Z |
| format | Article |
| id | doaj.art-514ca67a67404f75bb7dd3cc883aa0a4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T02:33:09Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-514ca67a67404f75bb7dd3cc883aa0a42022-12-21T19:56:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014294510.1371/journal.pone.0142945Association of Early Atherosclerosis with Vascular Wall Shear Stress in Hypercholesterolemic Zebrafish.Sang Joon LeeWoorak ChoiEunseok SeoEunseop YeomAlthough atherosclerosis is a multifactorial disease, the role of hemodynamic information has become more important. Low and oscillating wall shear stress (WSS) that changes its direction is associated with the early stage of atherosclerosis. Several in vitro and in vivo models were proposed to reveal the relation between the WSS and the early atherosclerosis. However, these models possess technical limitations in mimicking real physiological conditions and monitoring the developmental course of the early atherosclerosis. In this study, a hypercholesterolaemic zebrafish model is proposed as a novel experimental model to resolve these limitations. Zebrafish larvae are optically transparent, which enables temporal observation of pathological variations under in vivo condition. WSS in blood vessels of 15 days post-fertilisation zebrafish was measured using a micro particle image velocimetry (PIV) technique, and spatial distribution of lipid deposition inside the model was quantitatively investigated after feeding high cholesterol diet for 10 days. Lipids were mainly deposited in blood vessel of low WSS. The oscillating WSS was not induced by the blood flows in zebrafish models. The present hypercholesterolaemic zebrafish would be used as a potentially useful model for in vivo study about the effects of low WSS in the early atherosclerosis.http://europepmc.org/articles/PMC4643039?pdf=render |
| spellingShingle | Sang Joon Lee Woorak Choi Eunseok Seo Eunseop Yeom Association of Early Atherosclerosis with Vascular Wall Shear Stress in Hypercholesterolemic Zebrafish. PLoS ONE |
| title | Association of Early Atherosclerosis with Vascular Wall Shear Stress in Hypercholesterolemic Zebrafish. |
| title_full | Association of Early Atherosclerosis with Vascular Wall Shear Stress in Hypercholesterolemic Zebrafish. |
| title_fullStr | Association of Early Atherosclerosis with Vascular Wall Shear Stress in Hypercholesterolemic Zebrafish. |
| title_full_unstemmed | Association of Early Atherosclerosis with Vascular Wall Shear Stress in Hypercholesterolemic Zebrafish. |
| title_short | Association of Early Atherosclerosis with Vascular Wall Shear Stress in Hypercholesterolemic Zebrafish. |
| title_sort | association of early atherosclerosis with vascular wall shear stress in hypercholesterolemic zebrafish |
| url | http://europepmc.org/articles/PMC4643039?pdf=render |
| work_keys_str_mv | AT sangjoonlee associationofearlyatherosclerosiswithvascularwallshearstressinhypercholesterolemiczebrafish AT woorakchoi associationofearlyatherosclerosiswithvascularwallshearstressinhypercholesterolemiczebrafish AT eunseokseo associationofearlyatherosclerosiswithvascularwallshearstressinhypercholesterolemiczebrafish AT eunseopyeom associationofearlyatherosclerosiswithvascularwallshearstressinhypercholesterolemiczebrafish |