The effects of human parvovirus VP1 unique region in a mouse model of allergic asthma.
Evidence has indicated that viral infection increases the risk of developing asthma. Although the association of human parvovirus B19 (B19V) or human bocavirus (HBoV) with respiratory diseases has been reported, little is known about the influence of the B19V-VP1u and HBoV-VP1u proteins on the sympt...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0216799 |
| _version_ | 1818404127279939584 |
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| author | Shyh-Ren Chiang Chia-Yun Lin Der-Yuan Chen Hui-Fang Tsai Xin-Ci Lin Tsai-Ching Hsu Bor-Show Tzang |
| author_facet | Shyh-Ren Chiang Chia-Yun Lin Der-Yuan Chen Hui-Fang Tsai Xin-Ci Lin Tsai-Ching Hsu Bor-Show Tzang |
| author_sort | Shyh-Ren Chiang |
| collection | DOAJ |
| description | Evidence has indicated that viral infection increases the risk of developing asthma. Although the association of human parvovirus B19 (B19V) or human bocavirus (HBoV) with respiratory diseases has been reported, little is known about the influence of the B19V-VP1u and HBoV-VP1u proteins on the symptoms of asthma. Herein, we investigated the systemic influence of subcutaneously injected B19V-VP1u and HBoV-VP1u recombinant proteins in an OVA-sensitized asthmatic mouse model. A significantly higher Penh ratio and IgE level were detected in the serum, bronchoalveolar lavage fluid (BALF) and the supernatant of a lymphocyte culture from mice treated with HBoV-VP1u or B19V-VP1u than in a lymphocyte culture from OVA-sensitized mice. Significantly higher levels of serum and BALF IgE, total IgG, IgG1, OVA-specific IgE and OVA-specific IgG1 were detected in mice treated with HBoV-VP1u or B19V-VP1u than in OVA-sensitized mice. Conversely, a significantly lower IgG2a level was detected in mice from the HBoV-VP1u or B19V-VP1u groups than in mice from the OVA group. The mice treated with HBoV-VP1u or B19V-VP1u exhibited more significant lung inflammatory indices, including elevated serum and BALF IL-4, IL-5, IL-10 and IL-13 levels; BALF lymphocyte, neutrophil and eosinophil counts, MMP-9 and MMP-2 activity; and the amount of lymphocyte infiltration, relative to those in the control mice or in those sensitized with OVA. These findings demonstrate that the subcutaneous injection of HBoV-VP1u or B19V-VP1u proteins in OVA-sensitized mice result in elevated asthmatic indices and suggest that human parvoviruses may increase the risk of developing airway inflammation in a mouse model of asthma. |
| first_indexed | 2024-12-14T08:35:13Z |
| format | Article |
| id | doaj.art-514ce7f24dbf433a80583550666abcad |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T08:35:13Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-514ce7f24dbf433a80583550666abcad2022-12-21T23:09:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01145e021679910.1371/journal.pone.0216799The effects of human parvovirus VP1 unique region in a mouse model of allergic asthma.Shyh-Ren ChiangChia-Yun LinDer-Yuan ChenHui-Fang TsaiXin-Ci LinTsai-Ching HsuBor-Show TzangEvidence has indicated that viral infection increases the risk of developing asthma. Although the association of human parvovirus B19 (B19V) or human bocavirus (HBoV) with respiratory diseases has been reported, little is known about the influence of the B19V-VP1u and HBoV-VP1u proteins on the symptoms of asthma. Herein, we investigated the systemic influence of subcutaneously injected B19V-VP1u and HBoV-VP1u recombinant proteins in an OVA-sensitized asthmatic mouse model. A significantly higher Penh ratio and IgE level were detected in the serum, bronchoalveolar lavage fluid (BALF) and the supernatant of a lymphocyte culture from mice treated with HBoV-VP1u or B19V-VP1u than in a lymphocyte culture from OVA-sensitized mice. Significantly higher levels of serum and BALF IgE, total IgG, IgG1, OVA-specific IgE and OVA-specific IgG1 were detected in mice treated with HBoV-VP1u or B19V-VP1u than in OVA-sensitized mice. Conversely, a significantly lower IgG2a level was detected in mice from the HBoV-VP1u or B19V-VP1u groups than in mice from the OVA group. The mice treated with HBoV-VP1u or B19V-VP1u exhibited more significant lung inflammatory indices, including elevated serum and BALF IL-4, IL-5, IL-10 and IL-13 levels; BALF lymphocyte, neutrophil and eosinophil counts, MMP-9 and MMP-2 activity; and the amount of lymphocyte infiltration, relative to those in the control mice or in those sensitized with OVA. These findings demonstrate that the subcutaneous injection of HBoV-VP1u or B19V-VP1u proteins in OVA-sensitized mice result in elevated asthmatic indices and suggest that human parvoviruses may increase the risk of developing airway inflammation in a mouse model of asthma.https://doi.org/10.1371/journal.pone.0216799 |
| spellingShingle | Shyh-Ren Chiang Chia-Yun Lin Der-Yuan Chen Hui-Fang Tsai Xin-Ci Lin Tsai-Ching Hsu Bor-Show Tzang The effects of human parvovirus VP1 unique region in a mouse model of allergic asthma. PLoS ONE |
| title | The effects of human parvovirus VP1 unique region in a mouse model of allergic asthma. |
| title_full | The effects of human parvovirus VP1 unique region in a mouse model of allergic asthma. |
| title_fullStr | The effects of human parvovirus VP1 unique region in a mouse model of allergic asthma. |
| title_full_unstemmed | The effects of human parvovirus VP1 unique region in a mouse model of allergic asthma. |
| title_short | The effects of human parvovirus VP1 unique region in a mouse model of allergic asthma. |
| title_sort | effects of human parvovirus vp1 unique region in a mouse model of allergic asthma |
| url | https://doi.org/10.1371/journal.pone.0216799 |
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