Effect of explant density and culture medium volume on <em>in vitro</em> propagation of ‘FHIA-21’ (AAAB) hybrid in Temporary Immersion System
The need of producing high quality planting material has required the searching of new alternatives to increase the efficiency of in vitro propagation methods and their automation, such as Temporary Immersion Systems. This work has been carried out to increase the multiplication coefficient in mass...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2007-01-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/357 |
| _version_ | 1818515768588894208 |
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| author | Milagros Basail Pérez Rafael Gómez-Kosky Victor Medero Vega Eneida Otero Gálvez Marlenys Torres Delgado Manuel Cabrera Jova Jorge López Torres Magaly García García Arletys Santos Pino Aymé Rayas Cabrera José de la C. Ventura Martín Maricel Bauta Toledo Miguel Álvarez Mesa Eriker Páz Chávez Yoel Beovidez García Julia Albert Llerena Alexis Ortega Ortiz Alberto Espinosa Cuéllar Jesús García Ruiz |
| author_facet | Milagros Basail Pérez Rafael Gómez-Kosky Victor Medero Vega Eneida Otero Gálvez Marlenys Torres Delgado Manuel Cabrera Jova Jorge López Torres Magaly García García Arletys Santos Pino Aymé Rayas Cabrera José de la C. Ventura Martín Maricel Bauta Toledo Miguel Álvarez Mesa Eriker Páz Chávez Yoel Beovidez García Julia Albert Llerena Alexis Ortega Ortiz Alberto Espinosa Cuéllar Jesús García Ruiz |
| author_sort | Milagros Basail Pérez |
| collection | DOAJ |
| description | The need of producing high quality planting material has required the searching of new alternatives to increase the efficiency of in vitro propagation methods and their automation, such as Temporary Immersion Systems. This work has been carried out to increase the multiplication coefficient in mass propagation of hybrid ‘FHIA-21’ (AAAB) in Temporary Immersion Systems. Different culture medium volumes per explant and densities of planting materials per unit were studied at the same immersion frequency. The highest multiplication coefficient rate, (14.99 and 16.02 respectively), was obtained when 40 ml culture medium volume were used at a density of 70 explants/flask. The use of Temporary Immersion System allowed increasing the multiplication coefficient in hybrid ‘FHIA-21’ (AAAB) and the highest quality multiplication coefficient for rooting stage and further acclimatization in field conditions.
Key words: hybrid, liquid culture medium, multiplication coefficient |
| first_indexed | 2024-12-11T00:33:11Z |
| format | Article |
| id | doaj.art-514d21b57b0c4c66b1e87ca864847a18 |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-11T00:33:11Z |
| publishDate | 2007-01-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-514d21b57b0c4c66b1e87ca864847a182022-12-22T01:27:16ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472007-01-0171330Effect of explant density and culture medium volume on <em>in vitro</em> propagation of ‘FHIA-21’ (AAAB) hybrid in Temporary Immersion SystemMilagros Basail PérezRafael Gómez-KoskyVictor Medero VegaEneida Otero GálvezMarlenys Torres DelgadoManuel Cabrera JovaJorge López TorresMagaly García GarcíaArletys Santos PinoAymé Rayas CabreraJosé de la C. Ventura MartínMaricel Bauta ToledoMiguel Álvarez MesaEriker Páz ChávezYoel Beovidez GarcíaJulia Albert LlerenaAlexis Ortega OrtizAlberto Espinosa CuéllarJesús García RuizThe need of producing high quality planting material has required the searching of new alternatives to increase the efficiency of in vitro propagation methods and their automation, such as Temporary Immersion Systems. This work has been carried out to increase the multiplication coefficient in mass propagation of hybrid ‘FHIA-21’ (AAAB) in Temporary Immersion Systems. Different culture medium volumes per explant and densities of planting materials per unit were studied at the same immersion frequency. The highest multiplication coefficient rate, (14.99 and 16.02 respectively), was obtained when 40 ml culture medium volume were used at a density of 70 explants/flask. The use of Temporary Immersion System allowed increasing the multiplication coefficient in hybrid ‘FHIA-21’ (AAAB) and the highest quality multiplication coefficient for rooting stage and further acclimatization in field conditions. Key words: hybrid, liquid culture medium, multiplication coefficienthttps://revista.ibp.co.cu/index.php/BV/article/view/357 |
| spellingShingle | Milagros Basail Pérez Rafael Gómez-Kosky Victor Medero Vega Eneida Otero Gálvez Marlenys Torres Delgado Manuel Cabrera Jova Jorge López Torres Magaly García García Arletys Santos Pino Aymé Rayas Cabrera José de la C. Ventura Martín Maricel Bauta Toledo Miguel Álvarez Mesa Eriker Páz Chávez Yoel Beovidez García Julia Albert Llerena Alexis Ortega Ortiz Alberto Espinosa Cuéllar Jesús García Ruiz Effect of explant density and culture medium volume on <em>in vitro</em> propagation of ‘FHIA-21’ (AAAB) hybrid in Temporary Immersion System Biotecnología Vegetal |
| title | Effect of explant density and culture medium volume on <em>in vitro</em> propagation of ‘FHIA-21’ (AAAB) hybrid in Temporary Immersion System |
| title_full | Effect of explant density and culture medium volume on <em>in vitro</em> propagation of ‘FHIA-21’ (AAAB) hybrid in Temporary Immersion System |
| title_fullStr | Effect of explant density and culture medium volume on <em>in vitro</em> propagation of ‘FHIA-21’ (AAAB) hybrid in Temporary Immersion System |
| title_full_unstemmed | Effect of explant density and culture medium volume on <em>in vitro</em> propagation of ‘FHIA-21’ (AAAB) hybrid in Temporary Immersion System |
| title_short | Effect of explant density and culture medium volume on <em>in vitro</em> propagation of ‘FHIA-21’ (AAAB) hybrid in Temporary Immersion System |
| title_sort | effect of explant density and culture medium volume on em in vitro em propagation of fhia 21 aaab hybrid in temporary immersion system |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/357 |
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