Identifying essential long non-coding RNAs in cancer using CRISPRi-based dropout screens

Identifying essential long non-coding RNAs in cancer using CRISPRi-based dropout screens

Summary: Long non-coding RNAs (lncRNAs) are emerging as key regulators in the initiation, growth, and progression of cancer. High-throughput CRISPR-based techniques systematically assess the function of genes or regulatory elements present in the human genome. Here, we present a protocol for identif...

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Bibliographic Details
Main Authors: Oscar Bril, Laura J. Schwarzmueller, Leandro F. Moreno, Louis Vermeulen, Nicolas Léveillé
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:STAR Protocols
Subjects:
Bioinformatics
Cell Biology
Genomics
Molecular Biology
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723005555

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http://www.sciencedirect.com/science/article/pii/S2666166723005555

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