Prevalence of extended-spectrum and AmpC β-lactamase-producing Escherichia coli in young calves on Dutch dairy farms
ABSTRACT: In young calves on dairy farms the animal prevalence of extended-spectrum and AmpC β-lactamase-producing Escherichia coli (ESBL/AmpC-EC) is significantly higher compared with the animal prevalence in young stock and dairy cows. Hitherto it was unknown at what age antimicrobial resistant ba...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030223001522 |
| _version_ | 1797818347635605504 |
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| author | M.A. Gonggrijp A.G.J. Velthuis A.E. Heuvelink K.W.H. van den Heuvel C.C. ter Bogt-Kappert G.J. Buter G. van Schaik T.J.G.M. Lam |
| author_facet | M.A. Gonggrijp A.G.J. Velthuis A.E. Heuvelink K.W.H. van den Heuvel C.C. ter Bogt-Kappert G.J. Buter G. van Schaik T.J.G.M. Lam |
| author_sort | M.A. Gonggrijp |
| collection | DOAJ |
| description | ABSTRACT: In young calves on dairy farms the animal prevalence of extended-spectrum and AmpC β-lactamase-producing Escherichia coli (ESBL/AmpC-EC) is significantly higher compared with the animal prevalence in young stock and dairy cows. Hitherto it was unknown at what age antimicrobial resistant bacteria appear for the first time in the gut of calves on dairy farms, and how long these infections persist. The aim of this study was to examine the prevalence of ESBL/AmpC-EC, the number of excreted ESBL/AmpC-EC (in cfu/g of feces), as well as the ESBL/AmpC genotypes in young dairy calves (0–21 d of age) and the variation of these parameters between calves of different ages. Next to this, the course of shedding ESBL/AmpC-EC during the first year in dairy calves was studied. In a cross-sectional study, fecal samples from 748 calves, from 0 to 88 d of age, on 188 Dutch dairy farms were collected. The prevalence of calves testing positive for ESBL/AmpC-EC in a phenotypic assay was determined for different age categories (per 2 d of age). Positive samples were subjected to a semiquantitative test to determine the numbers of ESBL/AmpC-EC per gram of feces and for a selection of ESBL/AmpC-EC isolates the ESBL/AmpC genotype was determined. Ten of the 188 farms were selected for a longitudinal study based on the presence of at least 1 female calf with ESBL/Amp-EC in the cross-sectional study. These farms were additionally visited 3 times with a 4-mo interval. All calves that were sampled in the cross-sectional study were, if still present, resampled during the follow-up visits. Results show that from the day of birth ESBL/AmpC-EC can be present in the gut of calves. The phenotypic prevalence of ESBL/AmpC-EC was 33.3% in 0- to 21-d-old calves and 28.4% in 22- to 88-d-old calves. The prevalence of ESBL/AmpC-EC positive calves varied per age category among calves up to 21 d of age: significant increases and decreases at an early age were shown. Results of the longitudinal study show that after 4, 8, and 12 mo the prevalence of ESBL/AmpC-EC positive calves dropped to 3.8% (2/53), 5.8% (3/52), and 2.0% (1/49), respectively. This indicates that early gut colonization in young calves with ESBL/AmpC-EC is transient and does not lead to long-term shedding of these bacteria. |
| first_indexed | 2024-03-13T09:06:44Z |
| format | Article |
| id | doaj.art-514f44cc43e2403fa8d6d1f36abbaba4 |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-03-13T09:06:44Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-514f44cc43e2403fa8d6d1f36abbaba42023-05-28T04:08:25ZengElsevierJournal of Dairy Science0022-03022023-06-01106642574265Prevalence of extended-spectrum and AmpC β-lactamase-producing Escherichia coli in young calves on Dutch dairy farmsM.A. Gonggrijp0A.G.J. Velthuis1A.E. Heuvelink2K.W.H. van den Heuvel3C.C. ter Bogt-Kappert4G.J. Buter5G. van Schaik6T.J.G.M. Lam7Royal GD, PO Box 9, 7400 AA Deventer, the Netherlands; Corresponding authorRoyal GD, PO Box 9, 7400 AA Deventer, the Netherlands; Aeres University of Applied Sciences, Postbus 374, 8250 AJ, Dronten, the NetherlandsRoyal GD, PO Box 9, 7400 AA Deventer, the NetherlandsRoyal GD, PO Box 9, 7400 AA Deventer, the NetherlandsRoyal GD, PO Box 9, 7400 AA Deventer, the NetherlandsRoyal GD, PO Box 9, 7400 AA Deventer, the NetherlandsRoyal GD, PO Box 9, 7400 AA Deventer, the Netherlands; Department Population Health Sciences, Utrecht University, PO Box 80151, 3508 TD Utrecht, the NetherlandsRoyal GD, PO Box 9, 7400 AA Deventer, the Netherlands; Department Population Health Sciences, Utrecht University, PO Box 80151, 3508 TD Utrecht, the NetherlandsABSTRACT: In young calves on dairy farms the animal prevalence of extended-spectrum and AmpC β-lactamase-producing Escherichia coli (ESBL/AmpC-EC) is significantly higher compared with the animal prevalence in young stock and dairy cows. Hitherto it was unknown at what age antimicrobial resistant bacteria appear for the first time in the gut of calves on dairy farms, and how long these infections persist. The aim of this study was to examine the prevalence of ESBL/AmpC-EC, the number of excreted ESBL/AmpC-EC (in cfu/g of feces), as well as the ESBL/AmpC genotypes in young dairy calves (0–21 d of age) and the variation of these parameters between calves of different ages. Next to this, the course of shedding ESBL/AmpC-EC during the first year in dairy calves was studied. In a cross-sectional study, fecal samples from 748 calves, from 0 to 88 d of age, on 188 Dutch dairy farms were collected. The prevalence of calves testing positive for ESBL/AmpC-EC in a phenotypic assay was determined for different age categories (per 2 d of age). Positive samples were subjected to a semiquantitative test to determine the numbers of ESBL/AmpC-EC per gram of feces and for a selection of ESBL/AmpC-EC isolates the ESBL/AmpC genotype was determined. Ten of the 188 farms were selected for a longitudinal study based on the presence of at least 1 female calf with ESBL/Amp-EC in the cross-sectional study. These farms were additionally visited 3 times with a 4-mo interval. All calves that were sampled in the cross-sectional study were, if still present, resampled during the follow-up visits. Results show that from the day of birth ESBL/AmpC-EC can be present in the gut of calves. The phenotypic prevalence of ESBL/AmpC-EC was 33.3% in 0- to 21-d-old calves and 28.4% in 22- to 88-d-old calves. The prevalence of ESBL/AmpC-EC positive calves varied per age category among calves up to 21 d of age: significant increases and decreases at an early age were shown. Results of the longitudinal study show that after 4, 8, and 12 mo the prevalence of ESBL/AmpC-EC positive calves dropped to 3.8% (2/53), 5.8% (3/52), and 2.0% (1/49), respectively. This indicates that early gut colonization in young calves with ESBL/AmpC-EC is transient and does not lead to long-term shedding of these bacteria.http://www.sciencedirect.com/science/article/pii/S0022030223001522calvesantimicrobial resistanceESBL |
| spellingShingle | M.A. Gonggrijp A.G.J. Velthuis A.E. Heuvelink K.W.H. van den Heuvel C.C. ter Bogt-Kappert G.J. Buter G. van Schaik T.J.G.M. Lam Prevalence of extended-spectrum and AmpC β-lactamase-producing Escherichia coli in young calves on Dutch dairy farms Journal of Dairy Science calves antimicrobial resistance ESBL |
| title | Prevalence of extended-spectrum and AmpC β-lactamase-producing Escherichia coli in young calves on Dutch dairy farms |
| title_full | Prevalence of extended-spectrum and AmpC β-lactamase-producing Escherichia coli in young calves on Dutch dairy farms |
| title_fullStr | Prevalence of extended-spectrum and AmpC β-lactamase-producing Escherichia coli in young calves on Dutch dairy farms |
| title_full_unstemmed | Prevalence of extended-spectrum and AmpC β-lactamase-producing Escherichia coli in young calves on Dutch dairy farms |
| title_short | Prevalence of extended-spectrum and AmpC β-lactamase-producing Escherichia coli in young calves on Dutch dairy farms |
| title_sort | prevalence of extended spectrum and ampc β lactamase producing escherichia coli in young calves on dutch dairy farms |
| topic | calves antimicrobial resistance ESBL |
| url | http://www.sciencedirect.com/science/article/pii/S0022030223001522 |
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