Synthesis, characterization, and evaluation of mPEG–SN38 and mPEG–PLA–SN38 micelles for cancer therapy
Jing Xie,1 Xiaomin Zhang,2 Meiyu Teng,1 Bo Yu,2 Shuang Yang,1 Robert J Lee,1,3 Lesheng Teng1 1College of Life Sciences, Jilin University, Changchun, 2Hangzhou PushiKang Biotechnology Co., Ltd, Hangzhou, People’s Republic of China; 3Division of Pharmaceutics, College of Pharmacy, The Ohio...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2016-04-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/synthesis-characterization-and-evaluation-of-mpegndashsn38-and-mpegnda-peer-reviewed-article-IJN |
| _version_ | 1818858111898746880 |
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| author | Xie J Zhang X Teng M Yu B Yang S Lee RJ Teng L |
| author_facet | Xie J Zhang X Teng M Yu B Yang S Lee RJ Teng L |
| author_sort | Xie J |
| collection | DOAJ |
| description | Jing Xie,1 Xiaomin Zhang,2 Meiyu Teng,1 Bo Yu,2 Shuang Yang,1 Robert J Lee,1,3 Lesheng Teng1 1College of Life Sciences, Jilin University, Changchun, 2Hangzhou PushiKang Biotechnology Co., Ltd, Hangzhou, People’s Republic of China; 3Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH, USA Abstract: 7-Ethyl-10-hydroxy camptothecin (SN38) is a potent topoisomerase inhibitor and a metabolite of irinotecan. Its clinical development has been hampered by its poor solubility. To address this problem, methoxy poly(ethylene glycol)-2000 (mPEG2K)–SN38 and mPEG2K–poly(lactide) (PLA1.5K)–SN38 conjugates were prepared and then dispersed into an aqueous medium to form micelles. Physicochemical characteristics of SN38–polymer conjugate micelles, for example, micelle diameter, zeta potential, morphology, and drug content, were then evaluated. The results showed that the mean diameters of mPEG2K–SN38 and mPEG2K–PLA1.5K–SN38 micelles were ~130 and 20 nm, respectively. These two micelles had similar drug contents. mPEG2K–PLA1.5K–SN38 micelles were more homogeneous than mPEG2K–SN38 micelles. Moreover, in vitro drug release behavior of the micelles was studied by high performance liquid chromatography. SN38 release from mPEG2K–SN38 micelles was much faster than from mPEG2K–PLA1.5K–SN38 micelles. In vitro cytotoxicity, cellular uptake, and apoptosis assays of the SN38–polymer conjugate micelles were carried out on BEL-7402 human liver cancer cells. In vivo biodistribution and antitumor tumor efficacy studies were carried out in a nude mouse xenograft model derived from BEL-7402 cells. The results showed that mPEG2K–PLA1.5K–SN38 micelles were significantly more effective than mPEG2K–SN38 micelles in tumor inhibition, and the inhibitory effect of mPEG2K–PLA1.5K–SN38 micelles on tumor growth was significantly greater than that of mPEG2K–SN38 micelles (1,042 vs 1,837 mm) at 30 days. In conclusion, mPEG–PLA–SN38 is a promising anticancer agent that warrants further investigation. Keywords: SN38, polymer conjugate, micelles, chemotherapy, liver cancer |
| first_indexed | 2024-12-19T08:51:06Z |
| format | Article |
| id | doaj.art-514fbb14c4a5473583c9d8b96f540c25 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-19T08:51:06Z |
| publishDate | 2016-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-514fbb14c4a5473583c9d8b96f540c252022-12-21T20:28:44ZengDove Medical PressInternational Journal of Nanomedicine1178-20132016-04-012016default1677168626645Synthesis, characterization, and evaluation of mPEG–SN38 and mPEG–PLA–SN38 micelles for cancer therapyXie JZhang XTeng MYu BYang SLee RJTeng LJing Xie,1 Xiaomin Zhang,2 Meiyu Teng,1 Bo Yu,2 Shuang Yang,1 Robert J Lee,1,3 Lesheng Teng1 1College of Life Sciences, Jilin University, Changchun, 2Hangzhou PushiKang Biotechnology Co., Ltd, Hangzhou, People’s Republic of China; 3Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH, USA Abstract: 7-Ethyl-10-hydroxy camptothecin (SN38) is a potent topoisomerase inhibitor and a metabolite of irinotecan. Its clinical development has been hampered by its poor solubility. To address this problem, methoxy poly(ethylene glycol)-2000 (mPEG2K)–SN38 and mPEG2K–poly(lactide) (PLA1.5K)–SN38 conjugates were prepared and then dispersed into an aqueous medium to form micelles. Physicochemical characteristics of SN38–polymer conjugate micelles, for example, micelle diameter, zeta potential, morphology, and drug content, were then evaluated. The results showed that the mean diameters of mPEG2K–SN38 and mPEG2K–PLA1.5K–SN38 micelles were ~130 and 20 nm, respectively. These two micelles had similar drug contents. mPEG2K–PLA1.5K–SN38 micelles were more homogeneous than mPEG2K–SN38 micelles. Moreover, in vitro drug release behavior of the micelles was studied by high performance liquid chromatography. SN38 release from mPEG2K–SN38 micelles was much faster than from mPEG2K–PLA1.5K–SN38 micelles. In vitro cytotoxicity, cellular uptake, and apoptosis assays of the SN38–polymer conjugate micelles were carried out on BEL-7402 human liver cancer cells. In vivo biodistribution and antitumor tumor efficacy studies were carried out in a nude mouse xenograft model derived from BEL-7402 cells. The results showed that mPEG2K–PLA1.5K–SN38 micelles were significantly more effective than mPEG2K–SN38 micelles in tumor inhibition, and the inhibitory effect of mPEG2K–PLA1.5K–SN38 micelles on tumor growth was significantly greater than that of mPEG2K–SN38 micelles (1,042 vs 1,837 mm) at 30 days. In conclusion, mPEG–PLA–SN38 is a promising anticancer agent that warrants further investigation. Keywords: SN38, polymer conjugate, micelles, chemotherapy, liver cancerhttps://www.dovepress.com/synthesis-characterization-and-evaluation-of-mpegndashsn38-and-mpegnda-peer-reviewed-article-IJNSN38polymer conjugatemicelleschemotherapyliver cancer |
| spellingShingle | Xie J Zhang X Teng M Yu B Yang S Lee RJ Teng L Synthesis, characterization, and evaluation of mPEG–SN38 and mPEG–PLA–SN38 micelles for cancer therapy International Journal of Nanomedicine SN38 polymer conjugate micelles chemotherapy liver cancer |
| title | Synthesis, characterization, and evaluation of mPEG–SN38 and mPEG–PLA–SN38 micelles for cancer therapy |
| title_full | Synthesis, characterization, and evaluation of mPEG–SN38 and mPEG–PLA–SN38 micelles for cancer therapy |
| title_fullStr | Synthesis, characterization, and evaluation of mPEG–SN38 and mPEG–PLA–SN38 micelles for cancer therapy |
| title_full_unstemmed | Synthesis, characterization, and evaluation of mPEG–SN38 and mPEG–PLA–SN38 micelles for cancer therapy |
| title_short | Synthesis, characterization, and evaluation of mPEG–SN38 and mPEG–PLA–SN38 micelles for cancer therapy |
| title_sort | synthesis characterization and evaluation of mpeg ndash sn38 and mpeg ndash pla ndash sn38 micelles for cancer therapy |
| topic | SN38 polymer conjugate micelles chemotherapy liver cancer |
| url | https://www.dovepress.com/synthesis-characterization-and-evaluation-of-mpegndashsn38-and-mpegnda-peer-reviewed-article-IJN |
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