Preparation of Sulfated Xylans and Its in Vitro Proliferation of Probiotics
This study aimed to determine the effect of sulfated xylans on the growth of probiotics. Xylan (Xyl) was chemically modified using the sulfamic acid-N,N-dimethylformamide method to produce sulfated xylan (SXY). The SXY was characterized by Fourier infrared spectroscopy (FT-IR), scanning electron mic...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2023-07-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080112 |
| _version_ | 1797737839937454080 |
|---|---|
| author | Xia LI Qiying ZHANG Yuan GUAN Jing LI Haishan CHEN Lifen LI |
| author_facet | Xia LI Qiying ZHANG Yuan GUAN Jing LI Haishan CHEN Lifen LI |
| author_sort | Xia LI |
| collection | DOAJ |
| description | This study aimed to determine the effect of sulfated xylans on the growth of probiotics. Xylan (Xyl) was chemically modified using the sulfamic acid-N,N-dimethylformamide method to produce sulfated xylan (SXY). The SXY was characterized by Fourier infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and Congo red experiment. The Xyl and SXY were tested for their in vitro prebiotic effects using four strains of intestinal microflora, including Lactobacillus delbrueckii subsp. bulgaricus (GIM1.155), L. plantarum (GIM1.191), L. brevis (GIM1.773), and Streptococcus thermophilus (GIM1.540). From the recorded infrared spectra, vibrational bands for SXY were observed at 894, 1052 and 1243 cm−1, they could be assigned to the C—O—S vibration of a C—O—SO3, C—O stretched and S＝O stretching vibrations. The results of scanning electron microscopy showed that the surface structure of SXY increased smoothness. Congo red experiment revealed that SXY had a triple helix structure. The substitution degree of SXY measured using a BaCl2-gelatin turbidimeter was 0.341. In vitro experiment showed that the optimum concentration of SXY for the in vitro probiotic growth was 2.0%, rapid growth and increased proliferation were observed after 10 h of culture. The results obtained suggest that SXY could promote probiotic growth. It is an alternate source of prebiotics for maintaining gut health. |
| first_indexed | 2024-03-12T13:34:16Z |
| format | Article |
| id | doaj.art-5155bf7647104405a160412a477ca302 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:34:16Z |
| publishDate | 2023-07-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-5155bf7647104405a160412a477ca3022023-08-24T06:00:43ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-07-01441313414010.13386/j.issn1002-0306.20220801122022080112-13Preparation of Sulfated Xylans and Its in Vitro Proliferation of ProbioticsXia LI0Qiying ZHANG1Yuan GUAN2Jing LI3Haishan CHEN4Lifen LI5College of Chemistry and Bioengineering, Guilin University of Science and Technology, Guilin 541004, ChinaCollege of Chemistry and Bioengineering, Guilin University of Science and Technology, Guilin 541004, ChinaCollege of Chemistry and Bioengineering, Guilin University of Science and Technology, Guilin 541004, ChinaCollege of Chemistry and Bioengineering, Guilin University of Science and Technology, Guilin 541004, ChinaGuangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, ChinaCollege of Chemistry and Bioengineering, Guilin University of Science and Technology, Guilin 541004, ChinaThis study aimed to determine the effect of sulfated xylans on the growth of probiotics. Xylan (Xyl) was chemically modified using the sulfamic acid-N,N-dimethylformamide method to produce sulfated xylan (SXY). The SXY was characterized by Fourier infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and Congo red experiment. The Xyl and SXY were tested for their in vitro prebiotic effects using four strains of intestinal microflora, including Lactobacillus delbrueckii subsp. bulgaricus (GIM1.155), L. plantarum (GIM1.191), L. brevis (GIM1.773), and Streptococcus thermophilus (GIM1.540). From the recorded infrared spectra, vibrational bands for SXY were observed at 894, 1052 and 1243 cm−1, they could be assigned to the C—O—S vibration of a C—O—SO3, C—O stretched and S＝O stretching vibrations. The results of scanning electron microscopy showed that the surface structure of SXY increased smoothness. Congo red experiment revealed that SXY had a triple helix structure. The substitution degree of SXY measured using a BaCl2-gelatin turbidimeter was 0.341. In vitro experiment showed that the optimum concentration of SXY for the in vitro probiotic growth was 2.0%, rapid growth and increased proliferation were observed after 10 h of culture. The results obtained suggest that SXY could promote probiotic growth. It is an alternate source of prebiotics for maintaining gut health.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080112xylan (xyl)sulfationprobioticsin vitro proliferationprebiotics |
| spellingShingle | Xia LI Qiying ZHANG Yuan GUAN Jing LI Haishan CHEN Lifen LI Preparation of Sulfated Xylans and Its in Vitro Proliferation of Probiotics Shipin gongye ke-ji xylan (xyl) sulfation probiotics in vitro proliferation prebiotics |
| title | Preparation of Sulfated Xylans and Its in Vitro Proliferation of Probiotics |
| title_full | Preparation of Sulfated Xylans and Its in Vitro Proliferation of Probiotics |
| title_fullStr | Preparation of Sulfated Xylans and Its in Vitro Proliferation of Probiotics |
| title_full_unstemmed | Preparation of Sulfated Xylans and Its in Vitro Proliferation of Probiotics |
| title_short | Preparation of Sulfated Xylans and Its in Vitro Proliferation of Probiotics |
| title_sort | preparation of sulfated xylans and its in vitro proliferation of probiotics |
| topic | xylan (xyl) sulfation probiotics in vitro proliferation prebiotics |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022080112 |
| work_keys_str_mv | AT xiali preparationofsulfatedxylansanditsinvitroproliferationofprobiotics AT qiyingzhang preparationofsulfatedxylansanditsinvitroproliferationofprobiotics AT yuanguan preparationofsulfatedxylansanditsinvitroproliferationofprobiotics AT jingli preparationofsulfatedxylansanditsinvitroproliferationofprobiotics AT haishanchen preparationofsulfatedxylansanditsinvitroproliferationofprobiotics AT lifenli preparationofsulfatedxylansanditsinvitroproliferationofprobiotics |