Chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine
Objectives. To determine the toxicity and chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine (DMH). Materials and methods. The alkaloid extract was obtained from the fleshy part of M. bellavistensis, and an acu...
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| Format: | Article |
| Language: | Spanish |
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Instituto Nacional de Salud
2017-03-01
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| Series: | Revista Peruana de Medicina Experimental y Salud Pública |
| Subjects: | |
| Online Access: | https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2768 |
| _version_ | 1818457394743607296 |
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| author | Karla Ríos-León Cesar Fuertes-Ruiton Jorge Arroyo Julio Ruiz |
| author_facet | Karla Ríos-León Cesar Fuertes-Ruiton Jorge Arroyo Julio Ruiz |
| author_sort | Karla Ríos-León |
| collection | DOAJ |
| description | Objectives. To determine the toxicity and chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine (DMH). Materials and methods. The alkaloid extract was obtained from the fleshy part of M. bellavistensis, and an acute toxicity test was then carried out on 30 mice of the Balb C57 strain. To assess its chemoprotective effect, colon cancer was induced in 45 Holtzman rats using DMH according to the following experimental design: one control group received 2 mL/kg sodium polysorbate, and four groups received 20 mg/kg DMH plus 0, 1, 5, or 10 mg/kg M. bellavistensis alkaloid extract. Results. With a sample of 5 g of alkaloid extract, an LD50 greater than 1000 mg/mL was determined in the acute toxicity test. Histological indicators revealed that the 5 and 10 mg/kg doses had significant anti-tumor activity with 100% neoplasia inhibition against DMHinduced colon cancer in rats. Conclusions. Under experimental conditions, the alkaloid extract of M. bellavistensis has a chemoprotective effect against DMH-induced colon cancer in rats. |
| first_indexed | 2024-12-14T22:41:52Z |
| format | Article |
| id | doaj.art-5155fdd6c2804d96a5db73e3c294adc5 |
| institution | Directory Open Access Journal |
| issn | 1726-4634 1726-4642 |
| language | Spanish |
| last_indexed | 2024-12-14T22:41:52Z |
| publishDate | 2017-03-01 |
| publisher | Instituto Nacional de Salud |
| record_format | Article |
| series | Revista Peruana de Medicina Experimental y Salud Pública |
| spelling | doaj.art-5155fdd6c2804d96a5db73e3c294adc52022-12-21T22:44:57ZspaInstituto Nacional de SaludRevista Peruana de Medicina Experimental y Salud Pública1726-46341726-46422017-03-0134170510.17843/rpmesp.2017.341.27681866Chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazineKarla Ríos-León0Cesar Fuertes-Ruiton1Jorge Arroyo2Julio Ruiz3Instituto de Ciencias Farmacéuticas y Recursos Naturales, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Químico farmacéuticaInstituto de Ciencias Farmacéuticas y Recursos Naturales, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Químico farmacéutico, magíster en Ciencias Farmacéuticas y Recursos NaturalesInstituto de Investigaciones Clínicas, Facultad de Medicina, Universidad Nacional Mayor de San Marcos. Lima, Perú. Químico farmacéutico, doctor en Farmacia y BioquímicaInstituto de Investigación en Química Biológica, Microbiología y Biotecnología “Marco Antonio Garrido malo”. Lima, Perú. químico farmacéutico, magíster en Microbiología.Objectives. To determine the toxicity and chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine (DMH). Materials and methods. The alkaloid extract was obtained from the fleshy part of M. bellavistensis, and an acute toxicity test was then carried out on 30 mice of the Balb C57 strain. To assess its chemoprotective effect, colon cancer was induced in 45 Holtzman rats using DMH according to the following experimental design: one control group received 2 mL/kg sodium polysorbate, and four groups received 20 mg/kg DMH plus 0, 1, 5, or 10 mg/kg M. bellavistensis alkaloid extract. Results. With a sample of 5 g of alkaloid extract, an LD50 greater than 1000 mg/mL was determined in the acute toxicity test. Histological indicators revealed that the 5 and 10 mg/kg doses had significant anti-tumor activity with 100% neoplasia inhibition against DMHinduced colon cancer in rats. Conclusions. Under experimental conditions, the alkaloid extract of M. bellavistensis has a chemoprotective effect against DMH-induced colon cancer in rats.https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2768cáncer de colon, melocactus bellavistensis, alcaloides, 1,2-dimetilhidrazina, toxicidad, efecto quimioprotector |
| spellingShingle | Karla Ríos-León Cesar Fuertes-Ruiton Jorge Arroyo Julio Ruiz Chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine Revista Peruana de Medicina Experimental y Salud Pública cáncer de colon, melocactus bellavistensis, alcaloides, 1,2-dimetilhidrazina, toxicidad, efecto quimioprotector |
| title | Chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine |
| title_full | Chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine |
| title_fullStr | Chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine |
| title_full_unstemmed | Chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine |
| title_short | Chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine |
| title_sort | chemoprotective effect of the alkaloid extract of melocactus bellavistensis against colon cancer induced in rats using 1 2 dimethylhydrazine |
| topic | cáncer de colon, melocactus bellavistensis, alcaloides, 1,2-dimetilhidrazina, toxicidad, efecto quimioprotector |
| url | https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2768 |
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