Characterization of in vivo Dlg1 deletion on T cell development and function.
The polarized reorganization of the T cell membrane and intracellular signaling molecules in response to T cell receptor (TCR) engagement has been implicated in the modulation of T cell development and effector responses. In siRNA-based studies Dlg1, a MAGUK scaffold protein and member of the Scribb...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3445470?pdf=render |
| _version_ | 1819036034650865664 |
|---|---|
| author | Lisa A Humphries Meredith H Shaffer Faruk Sacirbegovic Tamar Tomassian Kerrie-Ann McMahon Patrick O Humbert Oscar Silva June L Round Kogo Takamiya Richard L Huganir Janis K Burkhardt Sarah M Russell M Carrie Miceli |
| author_facet | Lisa A Humphries Meredith H Shaffer Faruk Sacirbegovic Tamar Tomassian Kerrie-Ann McMahon Patrick O Humbert Oscar Silva June L Round Kogo Takamiya Richard L Huganir Janis K Burkhardt Sarah M Russell M Carrie Miceli |
| author_sort | Lisa A Humphries |
| collection | DOAJ |
| description | The polarized reorganization of the T cell membrane and intracellular signaling molecules in response to T cell receptor (TCR) engagement has been implicated in the modulation of T cell development and effector responses. In siRNA-based studies Dlg1, a MAGUK scaffold protein and member of the Scribble polarity complex, has been shown to play a role in T cell polarity and TCR signal specificity, however the role of Dlg1 in T cell development and function in vivo remains unclear.Here we present the combined data from three independently-derived dlg1-knockout mouse models; two germline deficient knockouts and one conditional knockout. While defects were not observed in T cell development, TCR-induced early phospho-signaling, actin-mediated events, or proliferation in any of the models, the acute knockdown of Dlg1 in Jurkat T cells diminished accumulation of actin at the IS. Further, while Th1-type cytokine production appeared unaffected in T cells derived from mice with a dlg1 germline-deficiency, altered production of TCR-dependent Th1 and Th2-type cytokines was observed in T cells derived from mice with a conditional loss of dlg1 expression and T cells with acute Dlg1 suppression, suggesting a differential requirement for Dlg1 activity in signaling events leading to Th1 versus Th2 cytokine induction. The observed inconsistencies between these and other knockout models and siRNA strategies suggest that 1) compensatory upregulation of alternate gene(s) may be masking a role for dlg1 in controlling TCR-mediated events in dlg1 deficient mice and 2) the developmental stage during which dlg1 ablation begins may control the degree to which compensatory events occur.These findings provide a potential explanation for the discrepancies observed in various studies using different dlg1-deficient T cell models and underscore the importance of acute dlg1 ablation to avoid the upregulation of compensatory mechanisms for future functional studies of the Dlg1 protein. |
| first_indexed | 2024-12-21T07:59:06Z |
| format | Article |
| id | doaj.art-5159c08438b74cfcb4ab902da3231388 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T07:59:06Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5159c08438b74cfcb4ab902da32313882022-12-21T19:10:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0179e4527610.1371/journal.pone.0045276Characterization of in vivo Dlg1 deletion on T cell development and function.Lisa A HumphriesMeredith H ShafferFaruk SacirbegovicTamar TomassianKerrie-Ann McMahonPatrick O HumbertOscar SilvaJune L RoundKogo TakamiyaRichard L HuganirJanis K BurkhardtSarah M RussellM Carrie MiceliThe polarized reorganization of the T cell membrane and intracellular signaling molecules in response to T cell receptor (TCR) engagement has been implicated in the modulation of T cell development and effector responses. In siRNA-based studies Dlg1, a MAGUK scaffold protein and member of the Scribble polarity complex, has been shown to play a role in T cell polarity and TCR signal specificity, however the role of Dlg1 in T cell development and function in vivo remains unclear.Here we present the combined data from three independently-derived dlg1-knockout mouse models; two germline deficient knockouts and one conditional knockout. While defects were not observed in T cell development, TCR-induced early phospho-signaling, actin-mediated events, or proliferation in any of the models, the acute knockdown of Dlg1 in Jurkat T cells diminished accumulation of actin at the IS. Further, while Th1-type cytokine production appeared unaffected in T cells derived from mice with a dlg1 germline-deficiency, altered production of TCR-dependent Th1 and Th2-type cytokines was observed in T cells derived from mice with a conditional loss of dlg1 expression and T cells with acute Dlg1 suppression, suggesting a differential requirement for Dlg1 activity in signaling events leading to Th1 versus Th2 cytokine induction. The observed inconsistencies between these and other knockout models and siRNA strategies suggest that 1) compensatory upregulation of alternate gene(s) may be masking a role for dlg1 in controlling TCR-mediated events in dlg1 deficient mice and 2) the developmental stage during which dlg1 ablation begins may control the degree to which compensatory events occur.These findings provide a potential explanation for the discrepancies observed in various studies using different dlg1-deficient T cell models and underscore the importance of acute dlg1 ablation to avoid the upregulation of compensatory mechanisms for future functional studies of the Dlg1 protein.http://europepmc.org/articles/PMC3445470?pdf=render |
| spellingShingle | Lisa A Humphries Meredith H Shaffer Faruk Sacirbegovic Tamar Tomassian Kerrie-Ann McMahon Patrick O Humbert Oscar Silva June L Round Kogo Takamiya Richard L Huganir Janis K Burkhardt Sarah M Russell M Carrie Miceli Characterization of in vivo Dlg1 deletion on T cell development and function. PLoS ONE |
| title | Characterization of in vivo Dlg1 deletion on T cell development and function. |
| title_full | Characterization of in vivo Dlg1 deletion on T cell development and function. |
| title_fullStr | Characterization of in vivo Dlg1 deletion on T cell development and function. |
| title_full_unstemmed | Characterization of in vivo Dlg1 deletion on T cell development and function. |
| title_short | Characterization of in vivo Dlg1 deletion on T cell development and function. |
| title_sort | characterization of in vivo dlg1 deletion on t cell development and function |
| url | http://europepmc.org/articles/PMC3445470?pdf=render |
| work_keys_str_mv | AT lisaahumphries characterizationofinvivodlg1deletionontcelldevelopmentandfunction AT meredithhshaffer characterizationofinvivodlg1deletionontcelldevelopmentandfunction AT faruksacirbegovic characterizationofinvivodlg1deletionontcelldevelopmentandfunction AT tamartomassian characterizationofinvivodlg1deletionontcelldevelopmentandfunction AT kerrieannmcmahon characterizationofinvivodlg1deletionontcelldevelopmentandfunction AT patrickohumbert characterizationofinvivodlg1deletionontcelldevelopmentandfunction AT oscarsilva characterizationofinvivodlg1deletionontcelldevelopmentandfunction AT junelround characterizationofinvivodlg1deletionontcelldevelopmentandfunction AT kogotakamiya characterizationofinvivodlg1deletionontcelldevelopmentandfunction AT richardlhuganir characterizationofinvivodlg1deletionontcelldevelopmentandfunction AT janiskburkhardt characterizationofinvivodlg1deletionontcelldevelopmentandfunction AT sarahmrussell characterizationofinvivodlg1deletionontcelldevelopmentandfunction AT mcarriemiceli characterizationofinvivodlg1deletionontcelldevelopmentandfunction |