Giant Sex Chromosomes in <i>Omophoita</i> Species (Coleoptera, Chrysomelidae): Structural and Evolutionary Relationships Revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH)
The beetles of the subtribe Oedionychina (Chrysomelidae, Alticinae) are the only ones that have the atypical giant and achiasmatic sex chromosomes, which are substantially larger than the autosomes. Previous cytogenetic analyses suggest a large accumulation of repetitive DNA in the sex chromosomes....
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MDPI AG
2023-05-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/5/440 |
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| author | Jhon A. D. Vidal Francisco de M. C. Sassi Renata L. R. de Moraes Roberto F. Artoni Thomas Liehr Marcelo B. Cioffi Mara C. de Almeida |
| author_facet | Jhon A. D. Vidal Francisco de M. C. Sassi Renata L. R. de Moraes Roberto F. Artoni Thomas Liehr Marcelo B. Cioffi Mara C. de Almeida |
| author_sort | Jhon A. D. Vidal |
| collection | DOAJ |
| description | The beetles of the subtribe Oedionychina (Chrysomelidae, Alticinae) are the only ones that have the atypical giant and achiasmatic sex chromosomes, which are substantially larger than the autosomes. Previous cytogenetic analyses suggest a large accumulation of repetitive DNA in the sex chromosomes. In this study, we examined the similarity of X and Y chromosomes in four <i>Omophoita</i> species and compared genomic differentiation to better understand the evolutionary process and the giant sex chromosomes origin. Intraspecific genomic comparation using male and female genomes of <i>O. octoguttata</i> and interespecific analyses using genomic DNA of <i>O. octoguttata</i>, <i>O. sexnotata</i>, <i>O. magniguttis</i>, and <i>O. personata</i> were performed. In addition, whole chromosome painting (WCP) experiments were performed with X and Y chromosome probes of <i>O. octogutatta</i>. CGH analysis revealed great genomic similarity between the sexes and a sex-specific region on the Y chromosome, and interspecific analysis revealed a genomic divergence between species. In contrast, WCP results revealed that the sex chromosomes of <i>O. octoguttata</i> have high intra- and interspecific similarity with the studied species. Our data support a common origin under the canonical evolution of the sex chromosomes in this group, as they have high genomic similarity between them. |
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| language | English |
| last_indexed | 2024-03-11T03:38:26Z |
| publishDate | 2023-05-01 |
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| series | Insects |
| spelling | doaj.art-515ab834e6fc4c6db4792b13418c4d5b2023-11-18T01:49:57ZengMDPI AGInsects2075-44502023-05-0114544010.3390/insects14050440Giant Sex Chromosomes in <i>Omophoita</i> Species (Coleoptera, Chrysomelidae): Structural and Evolutionary Relationships Revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH)Jhon A. D. Vidal0Francisco de M. C. Sassi1Renata L. R. de Moraes2Roberto F. Artoni3Thomas Liehr4Marcelo B. Cioffi5Mara C. de Almeida6Laboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos (UFSCar), Rodovia Washington Luiz Km. 235, C.P. 676, São Carlos 13565-905, BrazilLaboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos (UFSCar), Rodovia Washington Luiz Km. 235, C.P. 676, São Carlos 13565-905, BrazilLaboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos (UFSCar), Rodovia Washington Luiz Km. 235, C.P. 676, São Carlos 13565-905, BrazilLaboratório de Genética e Evolução, Departamento de Biologia Estrutural Molecular e Genética, Universidade Estadual de Ponta Grossa (UEPG), Av. Carlos Cavalcanti, 4748, Ponta Grossa 84030-900, BrazilInstitute of Human Genetics, University Hospital Jena, 07747 Jena, GermanyLaboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos (UFSCar), Rodovia Washington Luiz Km. 235, C.P. 676, São Carlos 13565-905, BrazilLaboratório de Genética e Evolução, Departamento de Biologia Estrutural Molecular e Genética, Universidade Estadual de Ponta Grossa (UEPG), Av. Carlos Cavalcanti, 4748, Ponta Grossa 84030-900, BrazilThe beetles of the subtribe Oedionychina (Chrysomelidae, Alticinae) are the only ones that have the atypical giant and achiasmatic sex chromosomes, which are substantially larger than the autosomes. Previous cytogenetic analyses suggest a large accumulation of repetitive DNA in the sex chromosomes. In this study, we examined the similarity of X and Y chromosomes in four <i>Omophoita</i> species and compared genomic differentiation to better understand the evolutionary process and the giant sex chromosomes origin. Intraspecific genomic comparation using male and female genomes of <i>O. octoguttata</i> and interespecific analyses using genomic DNA of <i>O. octoguttata</i>, <i>O. sexnotata</i>, <i>O. magniguttis</i>, and <i>O. personata</i> were performed. In addition, whole chromosome painting (WCP) experiments were performed with X and Y chromosome probes of <i>O. octogutatta</i>. CGH analysis revealed great genomic similarity between the sexes and a sex-specific region on the Y chromosome, and interspecific analysis revealed a genomic divergence between species. In contrast, WCP results revealed that the sex chromosomes of <i>O. octoguttata</i> have high intra- and interspecific similarity with the studied species. Our data support a common origin under the canonical evolution of the sex chromosomes in this group, as they have high genomic similarity between them.https://www.mdpi.com/2075-4450/14/5/440beetlesachiasmaticOedionychinaWCP |
| spellingShingle | Jhon A. D. Vidal Francisco de M. C. Sassi Renata L. R. de Moraes Roberto F. Artoni Thomas Liehr Marcelo B. Cioffi Mara C. de Almeida Giant Sex Chromosomes in <i>Omophoita</i> Species (Coleoptera, Chrysomelidae): Structural and Evolutionary Relationships Revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH) Insects beetles achiasmatic Oedionychina WCP |
| title | Giant Sex Chromosomes in <i>Omophoita</i> Species (Coleoptera, Chrysomelidae): Structural and Evolutionary Relationships Revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH) |
| title_full | Giant Sex Chromosomes in <i>Omophoita</i> Species (Coleoptera, Chrysomelidae): Structural and Evolutionary Relationships Revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH) |
| title_fullStr | Giant Sex Chromosomes in <i>Omophoita</i> Species (Coleoptera, Chrysomelidae): Structural and Evolutionary Relationships Revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH) |
| title_full_unstemmed | Giant Sex Chromosomes in <i>Omophoita</i> Species (Coleoptera, Chrysomelidae): Structural and Evolutionary Relationships Revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH) |
| title_short | Giant Sex Chromosomes in <i>Omophoita</i> Species (Coleoptera, Chrysomelidae): Structural and Evolutionary Relationships Revealed by Zoo-FISH and Comparative Genomic Hybridization (CGH) |
| title_sort | giant sex chromosomes in i omophoita i species coleoptera chrysomelidae structural and evolutionary relationships revealed by zoo fish and comparative genomic hybridization cgh |
| topic | beetles achiasmatic Oedionychina WCP |
| url | https://www.mdpi.com/2075-4450/14/5/440 |
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