Three-Dimensional Analysis of Round Window Membrane in the Chinchilla Model with Acute Otitis Media Induced with Streptococcus Pneumoniae 7F
Objective:The purpose of this study was to investigate the morphological changes of round window membrane (RWM) in chinchillas with Streptococcus pneumoniae (S. pneumoniae) serotype 7F induced acute otitis media (AOM) by two dimensional (2D) and three dimensional (3D) measurements.Methods:Temporal b...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Galenos Yayincilik
2021-03-01
|
| Series: | Turkish Archives of Otorhinolaryngology |
| Subjects: | |
| Online Access: |
http://turkarchotolaryngol.net/archives/archive-detail/article-preview/three-dimensional-analysis-of-round-window-membran/46912
|
| _version_ | 1828017251769384960 |
|---|---|
| author | Nevra Keskin Yılmaz Hasan Albasan Mehmet Kazım Börkü Michael Mauro Paparella Sebahattin Cüreoğlu |
| author_facet | Nevra Keskin Yılmaz Hasan Albasan Mehmet Kazım Börkü Michael Mauro Paparella Sebahattin Cüreoğlu |
| author_sort | Nevra Keskin Yılmaz |
| collection | DOAJ |
| description | Objective:The purpose of this study was to investigate the morphological changes of round window membrane (RWM) in chinchillas with Streptococcus pneumoniae (S. pneumoniae) serotype 7F induced acute otitis media (AOM) by two dimensional (2D) and three dimensional (3D) measurements.Methods:Temporal bone specimens taken from 12 chinchillas were divided into two groups. The control group consisted of healthy animals that were injected with intrabullar saline. The subjects in the experimental group were induced with AOM by intrabullar injection of S. pneumoniae 7F. The 2D and 3D measurements of RWM were compared between the groups.Results:Dramatic changes were noted in the RWM of the experimental group compared to the control group. The thickness [mean ± standard deviation (SD)] of the RWM was significantly (p<0.05) increased in the experimental group compared to the control group by 2D measurements taken at three different points of RWM. Moreover, 3D measurements revealed that the volume (mean ± SD) of RWM was significantly (p=0.009) increased in the experimental group.Conclusion:The results of our study, which indicated significant change in RWM in both 2D and 3D measurements, may shed light on the relationship between AOM and inner ear diseases. Based on our results, we recommend evaluating 3D analyses of RWM, which provide useful data, to better understand the changes in the membrane. |
| first_indexed | 2024-04-10T10:46:25Z |
| format | Article |
| id | doaj.art-515f1defebc64490a5c4fcd5830a4fc3 |
| institution | Directory Open Access Journal |
| issn | 2667-7474 |
| language | English |
| last_indexed | 2024-04-10T10:46:25Z |
| publishDate | 2021-03-01 |
| publisher | Galenos Yayincilik |
| record_format | Article |
| series | Turkish Archives of Otorhinolaryngology |
| spelling | doaj.art-515f1defebc64490a5c4fcd5830a4fc32023-02-15T16:20:17ZengGalenos YayincilikTurkish Archives of Otorhinolaryngology2667-74742021-03-01591434810.4274/tao.2021.599813049054Three-Dimensional Analysis of Round Window Membrane in the Chinchilla Model with Acute Otitis Media Induced with Streptococcus Pneumoniae 7FNevra Keskin Yılmaz0Hasan Albasan1Mehmet Kazım Börkü2Michael Mauro Paparella3Sebahattin Cüreoğlu4 Department of Internal Medicine, Ankara University, Faculty of Veterinary Medicine, Ankara, Turkey Pet Depot Veterinary Group, La Verne, California, USA Department of Internal Medicine, Ankara University, Faculty of Veterinary Medicine, Ankara, Turkey Department of Otorhinolaryngology, Head and Neck Surgery, University of Minnesota Medical School, Minneapolis/Minnesota, USA Department of Otorhinolaryngology, Head and Neck Surgery, University of Minnesota Medical School, Minneapolis/Minnesota, USA Objective:The purpose of this study was to investigate the morphological changes of round window membrane (RWM) in chinchillas with Streptococcus pneumoniae (S. pneumoniae) serotype 7F induced acute otitis media (AOM) by two dimensional (2D) and three dimensional (3D) measurements.Methods:Temporal bone specimens taken from 12 chinchillas were divided into two groups. The control group consisted of healthy animals that were injected with intrabullar saline. The subjects in the experimental group were induced with AOM by intrabullar injection of S. pneumoniae 7F. The 2D and 3D measurements of RWM were compared between the groups.Results:Dramatic changes were noted in the RWM of the experimental group compared to the control group. The thickness [mean ± standard deviation (SD)] of the RWM was significantly (p<0.05) increased in the experimental group compared to the control group by 2D measurements taken at three different points of RWM. Moreover, 3D measurements revealed that the volume (mean ± SD) of RWM was significantly (p=0.009) increased in the experimental group.Conclusion:The results of our study, which indicated significant change in RWM in both 2D and 3D measurements, may shed light on the relationship between AOM and inner ear diseases. Based on our results, we recommend evaluating 3D analyses of RWM, which provide useful data, to better understand the changes in the membrane. http://turkarchotolaryngol.net/archives/archive-detail/article-preview/three-dimensional-analysis-of-round-window-membran/46912 acute otitis mediapathologycochlear round windowstreptococcus pneumoniaecellular morphology |
| spellingShingle | Nevra Keskin Yılmaz Hasan Albasan Mehmet Kazım Börkü Michael Mauro Paparella Sebahattin Cüreoğlu Three-Dimensional Analysis of Round Window Membrane in the Chinchilla Model with Acute Otitis Media Induced with Streptococcus Pneumoniae 7F Turkish Archives of Otorhinolaryngology acute otitis media pathology cochlear round window streptococcus pneumoniae cellular morphology |
| title | Three-Dimensional Analysis of Round Window Membrane in the Chinchilla Model with Acute Otitis Media Induced with Streptococcus Pneumoniae 7F |
| title_full | Three-Dimensional Analysis of Round Window Membrane in the Chinchilla Model with Acute Otitis Media Induced with Streptococcus Pneumoniae 7F |
| title_fullStr | Three-Dimensional Analysis of Round Window Membrane in the Chinchilla Model with Acute Otitis Media Induced with Streptococcus Pneumoniae 7F |
| title_full_unstemmed | Three-Dimensional Analysis of Round Window Membrane in the Chinchilla Model with Acute Otitis Media Induced with Streptococcus Pneumoniae 7F |
| title_short | Three-Dimensional Analysis of Round Window Membrane in the Chinchilla Model with Acute Otitis Media Induced with Streptococcus Pneumoniae 7F |
| title_sort | three dimensional analysis of round window membrane in the chinchilla model with acute otitis media induced with streptococcus pneumoniae 7f |
| topic | acute otitis media pathology cochlear round window streptococcus pneumoniae cellular morphology |
| url |
http://turkarchotolaryngol.net/archives/archive-detail/article-preview/three-dimensional-analysis-of-round-window-membran/46912
|
| work_keys_str_mv | AT nevrakeskinyılmaz threedimensionalanalysisofroundwindowmembraneinthechinchillamodelwithacuteotitismediainducedwithstreptococcuspneumoniae7f AT hasanalbasan threedimensionalanalysisofroundwindowmembraneinthechinchillamodelwithacuteotitismediainducedwithstreptococcuspneumoniae7f AT mehmetkazımborku threedimensionalanalysisofroundwindowmembraneinthechinchillamodelwithacuteotitismediainducedwithstreptococcuspneumoniae7f AT michaelmauropaparella threedimensionalanalysisofroundwindowmembraneinthechinchillamodelwithacuteotitismediainducedwithstreptococcuspneumoniae7f AT sebahattincureoglu threedimensionalanalysisofroundwindowmembraneinthechinchillamodelwithacuteotitismediainducedwithstreptococcuspneumoniae7f |