Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes
Modified vaccinia Ankara (MVA) is a promising vaccine vector due to its highly attenuated phenotype and good immunogenicity. However, obtaining a new recombinant MVA remains a tedious and laborious procedure involving many rounds of plaque purification. Recombinant MVA generation can be greatly impr...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/3/528 |
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| author | Irina V. Antoshkina Dina V. Glazkova Felix A. Urusov Elena V. Bogoslovskaya German A. Shipulin |
| author_facet | Irina V. Antoshkina Dina V. Glazkova Felix A. Urusov Elena V. Bogoslovskaya German A. Shipulin |
| author_sort | Irina V. Antoshkina |
| collection | DOAJ |
| description | Modified vaccinia Ankara (MVA) is a promising vaccine vector due to its highly attenuated phenotype and good immunogenicity. However, obtaining a new recombinant MVA remains a tedious and laborious procedure involving many rounds of plaque purification. Recombinant MVA generation can be greatly improved and facilitated by different selection techniques. Here, we describe a comparison between techniques based on K1L, F13L and D4R genes. |
| first_indexed | 2024-03-09T12:16:44Z |
| format | Article |
| id | doaj.art-516069a442a14b0ca69f2fe51aa1318b |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T12:16:44Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-516069a442a14b0ca69f2fe51aa1318b2023-11-30T22:45:50ZengMDPI AGViruses1999-49152022-03-0114352810.3390/v14030528Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L GenesIrina V. Antoshkina0Dina V. Glazkova1Felix A. Urusov2Elena V. Bogoslovskaya3German A. Shipulin4Federal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, 119121 Moscow, RussiaFederal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, 119121 Moscow, RussiaFederal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, 119121 Moscow, RussiaFederal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, 119121 Moscow, RussiaFederal State Budgetary Institution «Centre for Strategic Planning and Management of Biomedical Health Risks» of the Federal Medical Biological Agency, 119121 Moscow, RussiaModified vaccinia Ankara (MVA) is a promising vaccine vector due to its highly attenuated phenotype and good immunogenicity. However, obtaining a new recombinant MVA remains a tedious and laborious procedure involving many rounds of plaque purification. Recombinant MVA generation can be greatly improved and facilitated by different selection techniques. Here, we describe a comparison between techniques based on K1L, F13L and D4R genes.https://www.mdpi.com/1999-4915/14/3/528MVAvaccinia virusrecombinant MVAhost-range selection |
| spellingShingle | Irina V. Antoshkina Dina V. Glazkova Felix A. Urusov Elena V. Bogoslovskaya German A. Shipulin Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes Viruses MVA vaccinia virus recombinant MVA host-range selection |
| title | Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes |
| title_full | Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes |
| title_fullStr | Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes |
| title_full_unstemmed | Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes |
| title_short | Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes |
| title_sort | comparison of recombinant mva selection methods based on f13l d4r and k1l genes |
| topic | MVA vaccinia virus recombinant MVA host-range selection |
| url | https://www.mdpi.com/1999-4915/14/3/528 |
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