Release of free fatty acids from Ehrlich ascites tumor cells
Ehrlich ascites tumor cells release free fatty acids (FFA) during in vitro incubation in media that contain albumin. The released FFA are derived by lipolysis from endogenous lipid esters. Addition of glucose to the incubation medium greatly decreases the quantity of fatty acid released by the cells...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1966-09-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520392464 |
| _version_ | 1818899212942704640 |
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| author | Arthur A. Spector Daniel Steinberg |
| author_facet | Arthur A. Spector Daniel Steinberg |
| author_sort | Arthur A. Spector |
| collection | DOAJ |
| description | Ehrlich ascites tumor cells release free fatty acids (FFA) during in vitro incubation in media that contain albumin. The released FFA are derived by lipolysis from endogenous lipid esters. Addition of glucose to the incubation medium greatly decreases the quantity of fatty acid released by the cells. Cyanide, which inhibits endogenous lipid oxidation but not lipolysis, increases the quantity of fatty acid released to media containing albumin and causes free fatty acid to accumulate in the cells in the absence of exogenous albumin. The release of fatty acid, either preformed or derived by lipolysis during prolonged incubations, occurs under conditions of net fatty acid uptake from the incubation medium. Net release of fatty acid from the cell occurs only when fatty acid-extracted albumin is present in the extracellular medium; extrapolation of the data suggests that net release will not occur under physiological conditions.It is postulated that free fatty acid uptake and release are independent processes, the direction of net fatty acid movement being determined by the relationship between cellular free fatty acid concentration (regulating efflux) and the molar ratio of free fatty acid to albumin in the extracellular medium (regulating uptake). |
| first_indexed | 2024-12-19T19:44:23Z |
| format | Article |
| id | doaj.art-5161fbff3805480b991504d507a4c13f |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-19T19:44:23Z |
| publishDate | 1966-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-5161fbff3805480b991504d507a4c13f2022-12-21T20:08:10ZengElsevierJournal of Lipid Research0022-22751966-09-0175649656Release of free fatty acids from Ehrlich ascites tumor cellsArthur A. Spector0Daniel Steinberg1Laboratory of Metabolism, National Heart Institute, National Institutes of Health, Bethesda, MarylandLaboratory of Metabolism, National Heart Institute, National Institutes of Health, Bethesda, MarylandEhrlich ascites tumor cells release free fatty acids (FFA) during in vitro incubation in media that contain albumin. The released FFA are derived by lipolysis from endogenous lipid esters. Addition of glucose to the incubation medium greatly decreases the quantity of fatty acid released by the cells. Cyanide, which inhibits endogenous lipid oxidation but not lipolysis, increases the quantity of fatty acid released to media containing albumin and causes free fatty acid to accumulate in the cells in the absence of exogenous albumin. The release of fatty acid, either preformed or derived by lipolysis during prolonged incubations, occurs under conditions of net fatty acid uptake from the incubation medium. Net release of fatty acid from the cell occurs only when fatty acid-extracted albumin is present in the extracellular medium; extrapolation of the data suggests that net release will not occur under physiological conditions.It is postulated that free fatty acid uptake and release are independent processes, the direction of net fatty acid movement being determined by the relationship between cellular free fatty acid concentration (regulating efflux) and the molar ratio of free fatty acid to albumin in the extracellular medium (regulating uptake).http://www.sciencedirect.com/science/article/pii/S0022227520392464free fatty acidtransportreleaseeffluxuptakelipid utilization |
| spellingShingle | Arthur A. Spector Daniel Steinberg Release of free fatty acids from Ehrlich ascites tumor cells Journal of Lipid Research free fatty acid transport release efflux uptake lipid utilization |
| title | Release of free fatty acids from Ehrlich ascites tumor cells |
| title_full | Release of free fatty acids from Ehrlich ascites tumor cells |
| title_fullStr | Release of free fatty acids from Ehrlich ascites tumor cells |
| title_full_unstemmed | Release of free fatty acids from Ehrlich ascites tumor cells |
| title_short | Release of free fatty acids from Ehrlich ascites tumor cells |
| title_sort | release of free fatty acids from ehrlich ascites tumor cells |
| topic | free fatty acid transport release efflux uptake lipid utilization |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520392464 |
| work_keys_str_mv | AT arthuraspector releaseoffreefattyacidsfromehrlichascitestumorcells AT danielsteinberg releaseoffreefattyacidsfromehrlichascitestumorcells |