Standardization of a protocol to irradiate cell cultures with low level laser
Introduction: It is impossible to apply orthodontic therapeutic laser during the orthodontic treatment and to select different times of exposure, frequency or intensity for every single cell. The whole area gets irradiated in seconds according to the laser type . This could have a proliferation eff...
| Main Authors: | , |
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| Format: | Article |
| Language: | Spanish |
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Universidad del Valle
2017-09-01
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| Series: | Revista Estomatologâia |
| Subjects: | |
| Online Access: | https://entornogeografico.univalle.edu.co/index.php/revista_estomatologia/article/view/5739 |
| Summary: | Introduction: It is impossible to apply orthodontic therapeutic laser during the orthodontic treatment and to select different times of exposure, frequency or intensity for every single cell. The whole area gets irradiated in seconds according to the laser type . This could have a proliferation effect in some cells and a harmful effect in others cells. So, it´s very important to asses the effects of a laser application on the cells involved in dental movement, to verify the effect and reaction after laser therapy.
Objective: To create and standarized a clinical protocol to irradiate culture cells involved in dental movement (Human fibroblasts, osteoblasts and pre-osteoclasts in order to understand the basics of it´s clinical effect.
Materials and methods: Gingival and periodontal fibroblasts, normal human osteoblasts (NHOst) and human osteoclast progenitor cells in culture were irradiated using a low energy level laser (As-GaAl), operated at 36.73mW, 832.79nm nm wavelength 3.75 J/cm2 and 32.40 seconds per well.
Conclusions: The cytotoxicity tests showed that irradiation is safe protocol for the four groups of cell cultures.
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| ISSN: | 0121-3873 2248-7220 |