Operating parameter optimization of cell surface hydrophobicity test for ureolytic bacteria
As one of the main non-covalent relations in microbiological-based systems, cell surface hydrophobicity (CSH) can be observed as a relevant parameter for biodegradation capability and suggested bacterial behaviour and biofilm formation during a bioremediation process. On the other hand, the role of...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2021-01-01
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| Series: | Journal of the Serbian Chemical Society |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2021/0352-51392000082S.pdf |
| _version_ | 1798023041367670784 |
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| author | Šovljanski Olja Lj. Pezo Lato L. Tomić Ana M. Ranitović Aleksandra S. Cvetković Dragoljub D. Markov Siniša L. |
| author_facet | Šovljanski Olja Lj. Pezo Lato L. Tomić Ana M. Ranitović Aleksandra S. Cvetković Dragoljub D. Markov Siniša L. |
| author_sort | Šovljanski Olja Lj. |
| collection | DOAJ |
| description | As one of the main non-covalent relations in microbiological-based systems, cell surface hydrophobicity (CSH) can be observed as a relevant parameter for biodegradation capability and suggested bacterial behaviour and biofilm formation during a bioremediation process. On the other hand, the role of ureolytic bacteria in bioremediation has subsequently led to the examination of this bacterial type in different engineering fields. In order to optimize the operating parameters of microbial adhesion to hydrocarbons test (MATH) for ureolytic bacteria, Box–Behnken experimental design was conducted for five ureolytic bacteria isolated from soils, as well as for the reference strain Sporosarcina pasteurii DSM 33. The optimization was completed with and without the essential substrate for the targeted metabolic reaction, with the aim to compare differences in bacterial hydrophobicity. A vortex time of 2 min, a hydrocarbon volume of 0.5 mL, and a phase separation time of 15 min are recommended as MATH operating parameters for all tested ureolytic bacteria. Although all bacteria are hydrophobic, lower CSH values in the presence of urea were observed for the same bacterium, which could be explained by the interaction of urea with the organic phase of the separation system, as well as a rapid ureolysis process that also occurs during the application of ureolytic bacteria in biotechnology systems. |
| first_indexed | 2024-04-11T17:39:44Z |
| format | Article |
| id | doaj.art-5166120cf7b6438f8b72d481127f9614 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-04-11T17:39:44Z |
| publishDate | 2021-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-5166120cf7b6438f8b72d481127f96142022-12-22T04:11:31ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212021-01-0186553354510.2298/JSC200813082S0352-51392000082SOperating parameter optimization of cell surface hydrophobicity test for ureolytic bacteriaŠovljanski Olja Lj.0https://orcid.org/0000-0002-9118-4209Pezo Lato L.1https://orcid.org/0000-0002-0704-3084Tomić Ana M.2https://orcid.org/0000-0002-6338-342XRanitović Aleksandra S.3Cvetković Dragoljub D.4Markov Siniša L.5University of Novi Sad, Faculty of Technology Novi Sad, Laboratory of Microbiology, Novi Sad, SerbiaUniversity of Belgrade, Institute of General and Physical Chemistry, Belgrade, SerbiaUniversity of Novi Sad, Faculty of Technology Novi Sad, Laboratory of Microbiology, Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Technology Novi Sad, Laboratory of Microbiology, Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Technology Novi Sad, Laboratory of Microbiology, Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Technology Novi Sad, Laboratory of Microbiology, Novi Sad, SerbiaAs one of the main non-covalent relations in microbiological-based systems, cell surface hydrophobicity (CSH) can be observed as a relevant parameter for biodegradation capability and suggested bacterial behaviour and biofilm formation during a bioremediation process. On the other hand, the role of ureolytic bacteria in bioremediation has subsequently led to the examination of this bacterial type in different engineering fields. In order to optimize the operating parameters of microbial adhesion to hydrocarbons test (MATH) for ureolytic bacteria, Box–Behnken experimental design was conducted for five ureolytic bacteria isolated from soils, as well as for the reference strain Sporosarcina pasteurii DSM 33. The optimization was completed with and without the essential substrate for the targeted metabolic reaction, with the aim to compare differences in bacterial hydrophobicity. A vortex time of 2 min, a hydrocarbon volume of 0.5 mL, and a phase separation time of 15 min are recommended as MATH operating parameters for all tested ureolytic bacteria. Although all bacteria are hydrophobic, lower CSH values in the presence of urea were observed for the same bacterium, which could be explained by the interaction of urea with the organic phase of the separation system, as well as a rapid ureolysis process that also occurs during the application of ureolytic bacteria in biotechnology systems.http://www.doiserbia.nb.rs/img/doi/0352-5139/2021/0352-51392000082S.pdfmath testadhesion potentialphase separation timehydrocarbon volumevortex timesporosarcina pasteurii |
| spellingShingle | Šovljanski Olja Lj. Pezo Lato L. Tomić Ana M. Ranitović Aleksandra S. Cvetković Dragoljub D. Markov Siniša L. Operating parameter optimization of cell surface hydrophobicity test for ureolytic bacteria Journal of the Serbian Chemical Society math test adhesion potential phase separation time hydrocarbon volume vortex time sporosarcina pasteurii |
| title | Operating parameter optimization of cell surface hydrophobicity test for ureolytic bacteria |
| title_full | Operating parameter optimization of cell surface hydrophobicity test for ureolytic bacteria |
| title_fullStr | Operating parameter optimization of cell surface hydrophobicity test for ureolytic bacteria |
| title_full_unstemmed | Operating parameter optimization of cell surface hydrophobicity test for ureolytic bacteria |
| title_short | Operating parameter optimization of cell surface hydrophobicity test for ureolytic bacteria |
| title_sort | operating parameter optimization of cell surface hydrophobicity test for ureolytic bacteria |
| topic | math test adhesion potential phase separation time hydrocarbon volume vortex time sporosarcina pasteurii |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2021/0352-51392000082S.pdf |
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