Analysis of lymphocyte T(CD4+) cells expression on severe early childhood caries and free caries
Early childhood caries (ECC) is still one of the many diseases found in children throughout the world. Cariogenic bacteria are a significant risk factor for ECC associated with early colonization and high levels of cariogenic microbes (Streptococcus mutans, S. mutans). Lymphocyte T (CD4+) cells know...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Infectious Disease Reports |
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| Online Access: | https://www.pagepress.org/journals/index.php/idr/article/view/8760 |
| _version_ | 1830353679419441152 |
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| author | Muhammad Luthfi Priyawan Rachmadi Aqsa Sjuhada Oki Retno Indrawati Agung Sosiawan Muhaimin Rifa’i |
| author_facet | Muhammad Luthfi Priyawan Rachmadi Aqsa Sjuhada Oki Retno Indrawati Agung Sosiawan Muhaimin Rifa’i |
| author_sort | Muhammad Luthfi |
| collection | DOAJ |
| description | Early childhood caries (ECC) is still one of the many diseases found in children throughout the world. Cariogenic bacteria are a significant risk factor for ECC associated with early colonization and high levels of cariogenic microbes (Streptococcus mutans, S. mutans). Lymphocyte T (CD4+) cells known as helper T cells, are effector cells for mediated host immunity. Naive T cells (CD4+) must be activated to initiate effector function. This activation occurs through interaction with professional antigen- presenting cells (pro-APC), especially dendritic cells that lead to intracellular pathways that regulate T cell receptor (TCR) more specifically against antigen in T cells. Lymphocyte cells from samples were collected from severe early childhood caries (S-ECC) and Free caries aged 5 to 6 years. The subjects were instructed to gargle 10 mL of sterile NaCl 1.5% solution for 30 seconds, and expectorate it into a sterile glass then analyzing T lymphocyte cell (CD4+) expression using flow cytometry. Lymphocyte T (CD4+) cell expression at SECC (6.2525±64482) while in free caries (8.4138±1.10397) with P-value (P=0. 000). Conclusion of lymphocyte T (CD4+) cells expression at S-ECC is lower than that occurring in free caries. |
| first_indexed | 2024-12-20T01:17:56Z |
| format | Article |
| id | doaj.art-5168b24228814938a63b18e3954bda56 |
| institution | Directory Open Access Journal |
| issn | 2036-7430 2036-7449 |
| language | English |
| last_indexed | 2024-12-20T01:17:56Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Infectious Disease Reports |
| spelling | doaj.art-5168b24228814938a63b18e3954bda562022-12-21T19:58:32ZengMDPI AGInfectious Disease Reports2036-74302036-74492020-07-01121s10.4081/idr.2020.8760Analysis of lymphocyte T(CD4+) cells expression on severe early childhood caries and free cariesMuhammad Luthfi0Priyawan Rachmadi1Aqsa Sjuhada Oki2Retno Indrawati3Agung Sosiawan4Muhaimin Rifa’i5Department of Oral Biology, Faculty of Dental Medicine Universitas Airlangga, SurabayaDepartment of Dental Material, Faculty of Dental Medicine Universitas Airlangga, SurabayaDepartment of Oral Biology, Faculty of Dental Medicine Universitas Airlangga, SurabayaDepartment of Oral Biology ,Faculty of Dental Medicine Universitas Airlangga, SurabayaDepartment of Public Health, Faculty of Dental Medicine Universitas Airlangga, SurabayaDepartment of Physiology, Cell Culture and Animal Develepment, Faculty of Sciences, Brawijaya University, MalangEarly childhood caries (ECC) is still one of the many diseases found in children throughout the world. Cariogenic bacteria are a significant risk factor for ECC associated with early colonization and high levels of cariogenic microbes (Streptococcus mutans, S. mutans). Lymphocyte T (CD4+) cells known as helper T cells, are effector cells for mediated host immunity. Naive T cells (CD4+) must be activated to initiate effector function. This activation occurs through interaction with professional antigen- presenting cells (pro-APC), especially dendritic cells that lead to intracellular pathways that regulate T cell receptor (TCR) more specifically against antigen in T cells. Lymphocyte cells from samples were collected from severe early childhood caries (S-ECC) and Free caries aged 5 to 6 years. The subjects were instructed to gargle 10 mL of sterile NaCl 1.5% solution for 30 seconds, and expectorate it into a sterile glass then analyzing T lymphocyte cell (CD4+) expression using flow cytometry. Lymphocyte T (CD4+) cell expression at SECC (6.2525±64482) while in free caries (8.4138±1.10397) with P-value (P=0. 000). Conclusion of lymphocyte T (CD4+) cells expression at S-ECC is lower than that occurring in free caries.https://www.pagepress.org/journals/index.php/idr/article/view/8760Severe early childhood cariesadaptive immunitylymphocyte T (CD4 ) cells expression |
| spellingShingle | Muhammad Luthfi Priyawan Rachmadi Aqsa Sjuhada Oki Retno Indrawati Agung Sosiawan Muhaimin Rifa’i Analysis of lymphocyte T(CD4+) cells expression on severe early childhood caries and free caries Infectious Disease Reports Severe early childhood caries adaptive immunity lymphocyte T (CD4 ) cells expression |
| title | Analysis of lymphocyte T(CD4+) cells expression on severe early childhood caries and free caries |
| title_full | Analysis of lymphocyte T(CD4+) cells expression on severe early childhood caries and free caries |
| title_fullStr | Analysis of lymphocyte T(CD4+) cells expression on severe early childhood caries and free caries |
| title_full_unstemmed | Analysis of lymphocyte T(CD4+) cells expression on severe early childhood caries and free caries |
| title_short | Analysis of lymphocyte T(CD4+) cells expression on severe early childhood caries and free caries |
| title_sort | analysis of lymphocyte t cd4 cells expression on severe early childhood caries and free caries |
| topic | Severe early childhood caries adaptive immunity lymphocyte T (CD4 ) cells expression |
| url | https://www.pagepress.org/journals/index.php/idr/article/view/8760 |
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