Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the <i>Pseudomonas aeruginosa</i> Clinical Strains
<i>Pseudomonas aeruginosa</i> is one of the most commonly isolated bacteria from clinical specimens, with an increasing isolation frequency in nosocomial outbreaks. The hypothesis tested was whether carbapenem-resistant <i>P. aeruginosa</i> strains display an altered carriage...
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MDPI AG
2021-02-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/3/241 |
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| author | Tomasz Bogiel Dagmara Depka Mateusz Rzepka Joanna Kwiecińska-Piróg Eugenia Gospodarek-Komkowska |
| author_facet | Tomasz Bogiel Dagmara Depka Mateusz Rzepka Joanna Kwiecińska-Piróg Eugenia Gospodarek-Komkowska |
| author_sort | Tomasz Bogiel |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> is one of the most commonly isolated bacteria from clinical specimens, with an increasing isolation frequency in nosocomial outbreaks. The hypothesis tested was whether carbapenem-resistant <i>P. aeruginosa</i> strains display an altered carriage of the virulence factor genes, depending on the type of carbapenem resistance. The aim of the study was to investigate, by PCR, the frequency of 10 chosen virulence factors genes (<i>phzM</i>, <i>phzS</i>, <i>exoT</i>, <i>exoY</i>, <i>exoU</i>, <i>toxA</i>, <i>exoS</i>, <i>algD</i>, <i>pilA</i> and <i>pilB</i>) and the genotype distribution in 107 non-duplicated carbapenem-resistant <i>P. aeruginosa</i> isolates. <i>P. aeruginosa</i> genes involved in phenazine dyes and exoenzyme T synthesis were noted with the highest frequency (100%). Fimbriae-encoding genes were detected with the lowest incidence: 15.9% and 4.7% for pilin A and B, respectively. The differences observed between the <i>exoS</i> gene prevalence amongst the carbapenemase-positive and the carbapenemase-negative strains and the <i>pilA</i> gene prevalence amongst the strains of different origins were statistically significant. Virulence genes’ prevalence and the genotype distribution vary amongst <i>P. aeruginosa</i> strains resistant to carbapenems, especially in terms of their carbapenemase synthesis ability and the strain origin. |
| first_indexed | 2024-03-09T06:14:37Z |
| format | Article |
| id | doaj.art-5168c2c6e1cb420aadc3a18e9fd3ad07 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T06:14:37Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-5168c2c6e1cb420aadc3a18e9fd3ad072023-12-03T11:54:51ZengMDPI AGAntibiotics2079-63822021-02-0110324110.3390/antibiotics10030241Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the <i>Pseudomonas aeruginosa</i> Clinical StrainsTomasz Bogiel0Dagmara Depka1Mateusz Rzepka2Joanna Kwiecińska-Piróg3Eugenia Gospodarek-Komkowska4Microbiology Department, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-094 Bydgoszcz, PolandMicrobiology Department, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-094 Bydgoszcz, PolandMicrobiology Department, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-094 Bydgoszcz, PolandMicrobiology Department, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-094 Bydgoszcz, PolandMicrobiology Department, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-094 Bydgoszcz, Poland<i>Pseudomonas aeruginosa</i> is one of the most commonly isolated bacteria from clinical specimens, with an increasing isolation frequency in nosocomial outbreaks. The hypothesis tested was whether carbapenem-resistant <i>P. aeruginosa</i> strains display an altered carriage of the virulence factor genes, depending on the type of carbapenem resistance. The aim of the study was to investigate, by PCR, the frequency of 10 chosen virulence factors genes (<i>phzM</i>, <i>phzS</i>, <i>exoT</i>, <i>exoY</i>, <i>exoU</i>, <i>toxA</i>, <i>exoS</i>, <i>algD</i>, <i>pilA</i> and <i>pilB</i>) and the genotype distribution in 107 non-duplicated carbapenem-resistant <i>P. aeruginosa</i> isolates. <i>P. aeruginosa</i> genes involved in phenazine dyes and exoenzyme T synthesis were noted with the highest frequency (100%). Fimbriae-encoding genes were detected with the lowest incidence: 15.9% and 4.7% for pilin A and B, respectively. The differences observed between the <i>exoS</i> gene prevalence amongst the carbapenemase-positive and the carbapenemase-negative strains and the <i>pilA</i> gene prevalence amongst the strains of different origins were statistically significant. Virulence genes’ prevalence and the genotype distribution vary amongst <i>P. aeruginosa</i> strains resistant to carbapenems, especially in terms of their carbapenemase synthesis ability and the strain origin.https://www.mdpi.com/2079-6382/10/3/241biofilmexotoxins<i>Pseudomonas aeruginosa</i> biofilm<i>Pseudomonas aeruginosa</i> virulencetoxinsvirulence |
| spellingShingle | Tomasz Bogiel Dagmara Depka Mateusz Rzepka Joanna Kwiecińska-Piróg Eugenia Gospodarek-Komkowska Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the <i>Pseudomonas aeruginosa</i> Clinical Strains Antibiotics biofilm exotoxins <i>Pseudomonas aeruginosa</i> biofilm <i>Pseudomonas aeruginosa</i> virulence toxins virulence |
| title | Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the <i>Pseudomonas aeruginosa</i> Clinical Strains |
| title_full | Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the <i>Pseudomonas aeruginosa</i> Clinical Strains |
| title_fullStr | Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the <i>Pseudomonas aeruginosa</i> Clinical Strains |
| title_full_unstemmed | Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the <i>Pseudomonas aeruginosa</i> Clinical Strains |
| title_short | Prevalence of the Genes Associated with Biofilm and Toxins Synthesis amongst the <i>Pseudomonas aeruginosa</i> Clinical Strains |
| title_sort | prevalence of the genes associated with biofilm and toxins synthesis amongst the i pseudomonas aeruginosa i clinical strains |
| topic | biofilm exotoxins <i>Pseudomonas aeruginosa</i> biofilm <i>Pseudomonas aeruginosa</i> virulence toxins virulence |
| url | https://www.mdpi.com/2079-6382/10/3/241 |
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