Immunohistochemical study of ki-67 and bcl-2 expression in some odontogenic cystic lesions with different clinical behaviors
Background: Cystic lesions with odontogenic epithelial origin and similar clinicoradiographic appearance, show different clinical behaviors. Objective: To compare some factors related to cell proliferation and escape from apoptosis in epithelium covering two groups of odontogenic cystic lesions wit...
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| Format: | Article |
| Language: | fas |
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Qazvin University of Medical Sciences & Health Services
2016-11-01
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| Series: | The Journal of Qazvin University of Medical Sciences |
| Subjects: | |
| Online Access: | http://journal.qums.ac.ir/browse.php?a_id=2112&sid=1&slc_lang=en |
| _version_ | 1818050051283353600 |
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| author | Seyed Hossein Tabatabaei Shokoh TaqipourZahir Samaneh Keshavarz Fatemeh Khajehzade |
| author_facet | Seyed Hossein Tabatabaei Shokoh TaqipourZahir Samaneh Keshavarz Fatemeh Khajehzade |
| author_sort | Seyed Hossein Tabatabaei |
| collection | DOAJ |
| description | Background: Cystic lesions with odontogenic epithelial origin and similar clinicoradiographic appearance, show different clinical behaviors.
Objective: To compare some factors related to cell proliferation and escape from apoptosis in epithelium covering two groups of odontogenic cystic lesions with different clinical behaviors.
Methods: In this cross-sectional study 11 paraffin-embedded samples were selected of each lesions radicular cyst, dentigerous cyst, odontogenic keratocyst, and unicystic ameloblastoma. The sample underwent immunohistochemical staining for investigating the expression of ki-67 antigen and bcl-2 protein. Data analyzed with SPSS17 software and Kruskal–Wallis and chi-square statistical tests.
Findings: Most of ki-67 positive cells were observed in parabasal layer of odontogenic keratocyst [35.50±26.29%; P=0.001]. The average of ki-67-LI was more in parabasal layer of aggressive group (26.80±37.79%) compared to non-aggressive group (4.04±3.38%), was not being statistically significant. The highest average of bcl-2-LI was 95±6.70% in basal layer of odontogenic keratocyst (P=0.001). In all layers, the average of bcl-2-LI was more in aggressive lesions compared to non-aggressive ones and the highest amount was found in basal layer (72.45±3.94×10%) which was statistically significant (P=0.001).
Conclusion: According to the results of this study, more expression of the markers related to escape from apoptosis in aggressive lesions group compared to non-aggressive group, suggests that escape from apoptosis had a more critical role in aggressive behavior of odontogenic cystic lesions. |
| first_indexed | 2024-12-10T10:47:19Z |
| format | Article |
| id | doaj.art-5169a1a6535949a2ad95ec4e495c1553 |
| institution | Directory Open Access Journal |
| issn | 1561-3666 2228-7213 |
| language | fas |
| last_indexed | 2024-12-10T10:47:19Z |
| publishDate | 2016-11-01 |
| publisher | Qazvin University of Medical Sciences & Health Services |
| record_format | Article |
| series | The Journal of Qazvin University of Medical Sciences |
| spelling | doaj.art-5169a1a6535949a2ad95ec4e495c15532022-12-22T01:52:07ZfasQazvin University of Medical Sciences & Health ServicesThe Journal of Qazvin University of Medical Sciences1561-36662228-72132016-11-01204412Immunohistochemical study of ki-67 and bcl-2 expression in some odontogenic cystic lesions with different clinical behaviorsSeyed Hossein Tabatabaei0Shokoh TaqipourZahir1Samaneh Keshavarz2Fatemeh Khajehzade3Department of Oral and Maxillofacial Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Clinical and Anatomical Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Oral and Maxillofacial Pathology, Rafsanjan University of Medical Science, Rafsanjan, IranDepartment of Oral and Maxillofacial Pathology, Yasooj University of Medical Science, Yasooj, IranBackground: Cystic lesions with odontogenic epithelial origin and similar clinicoradiographic appearance, show different clinical behaviors. Objective: To compare some factors related to cell proliferation and escape from apoptosis in epithelium covering two groups of odontogenic cystic lesions with different clinical behaviors. Methods: In this cross-sectional study 11 paraffin-embedded samples were selected of each lesions radicular cyst, dentigerous cyst, odontogenic keratocyst, and unicystic ameloblastoma. The sample underwent immunohistochemical staining for investigating the expression of ki-67 antigen and bcl-2 protein. Data analyzed with SPSS17 software and Kruskal–Wallis and chi-square statistical tests. Findings: Most of ki-67 positive cells were observed in parabasal layer of odontogenic keratocyst [35.50±26.29%; P=0.001]. The average of ki-67-LI was more in parabasal layer of aggressive group (26.80±37.79%) compared to non-aggressive group (4.04±3.38%), was not being statistically significant. The highest average of bcl-2-LI was 95±6.70% in basal layer of odontogenic keratocyst (P=0.001). In all layers, the average of bcl-2-LI was more in aggressive lesions compared to non-aggressive ones and the highest amount was found in basal layer (72.45±3.94×10%) which was statistically significant (P=0.001). Conclusion: According to the results of this study, more expression of the markers related to escape from apoptosis in aggressive lesions group compared to non-aggressive group, suggests that escape from apoptosis had a more critical role in aggressive behavior of odontogenic cystic lesions.http://journal.qums.ac.ir/browse.php?a_id=2112&sid=1&slc_lang=enbcl-2 Proteinki-67 AntigenImmunohistochemical StainingOdontogenic Cystic Lesions |
| spellingShingle | Seyed Hossein Tabatabaei Shokoh TaqipourZahir Samaneh Keshavarz Fatemeh Khajehzade Immunohistochemical study of ki-67 and bcl-2 expression in some odontogenic cystic lesions with different clinical behaviors The Journal of Qazvin University of Medical Sciences bcl-2 Protein ki-67 Antigen Immunohistochemical Staining Odontogenic Cystic Lesions |
| title | Immunohistochemical study of ki-67 and bcl-2 expression in some odontogenic cystic lesions with different clinical behaviors |
| title_full | Immunohistochemical study of ki-67 and bcl-2 expression in some odontogenic cystic lesions with different clinical behaviors |
| title_fullStr | Immunohistochemical study of ki-67 and bcl-2 expression in some odontogenic cystic lesions with different clinical behaviors |
| title_full_unstemmed | Immunohistochemical study of ki-67 and bcl-2 expression in some odontogenic cystic lesions with different clinical behaviors |
| title_short | Immunohistochemical study of ki-67 and bcl-2 expression in some odontogenic cystic lesions with different clinical behaviors |
| title_sort | immunohistochemical study of ki 67 and bcl 2 expression in some odontogenic cystic lesions with different clinical behaviors |
| topic | bcl-2 Protein ki-67 Antigen Immunohistochemical Staining Odontogenic Cystic Lesions |
| url | http://journal.qums.ac.ir/browse.php?a_id=2112&sid=1&slc_lang=en |
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