Antibiotic Treatment in <i>Anopheles coluzzii</i> Affects Carbon and Nitrogen Metabolism
The mosquito microbiota reduces the vector competence of <i>Anopheles</i> to <i>Plasmodium</i> and affects host fitness; it is therefore considered as a potential target to reduce malaria transmission. While immune induction, secretion of antimicrobials and metabolic competit...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/9/679 |
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| author | Estelle Chabanol Volker Behrends Ghislaine Prévot George K. Christophides Mathilde Gendrin |
| author_facet | Estelle Chabanol Volker Behrends Ghislaine Prévot George K. Christophides Mathilde Gendrin |
| author_sort | Estelle Chabanol |
| collection | DOAJ |
| description | The mosquito microbiota reduces the vector competence of <i>Anopheles</i> to <i>Plasmodium</i> and affects host fitness; it is therefore considered as a potential target to reduce malaria transmission. While immune induction, secretion of antimicrobials and metabolic competition are three typical mechanisms of microbiota-mediated protection against invasive pathogens in mammals, the involvement of metabolic competition or mutualism in mosquito-microbiota and microbiota-<i>Plasmodium</i> interactions has not been investigated. Here, we describe a metabolome analysis of the midgut of <i>Anopheles coluzzii</i> provided with a sugar-meal or a non-infectious blood-meal, under conventional or antibiotic-treated conditions. We observed that the antibiotic treatment affects the tricarboxylic acid cycle and nitrogen metabolism, notably resulting in decreased abundance of free amino acids. Linking our results with published data, we identified pathways which may participate in microbiota-<i>Plasmodium</i> interactions via metabolic interactions or immune modulation and thus would be interesting candidates for future functional studies. |
| first_indexed | 2024-03-10T17:04:46Z |
| format | Article |
| id | doaj.art-516aafd77c5549fd89b9fb55368895f7 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T17:04:46Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-516aafd77c5549fd89b9fb55368895f72023-11-20T10:51:11ZengMDPI AGPathogens2076-08172020-08-019967910.3390/pathogens9090679Antibiotic Treatment in <i>Anopheles coluzzii</i> Affects Carbon and Nitrogen MetabolismEstelle Chabanol0Volker Behrends1Ghislaine Prévot2George K. Christophides3Mathilde Gendrin4Microbiota of Insect Vectors Group, Institut Pasteur de Guyane, 97306 Cayenne, French GuianaHealth Sciences Research Centre, University of Roehampton, London SW15 4JD, UKTropical Biome and Immunophysiopathology, Université de Guyane, 97300 Cayenne, French GuianaDepartment of Life Sciences, Imperial College London, London SW7 2BU, UKMicrobiota of Insect Vectors Group, Institut Pasteur de Guyane, 97306 Cayenne, French GuianaThe mosquito microbiota reduces the vector competence of <i>Anopheles</i> to <i>Plasmodium</i> and affects host fitness; it is therefore considered as a potential target to reduce malaria transmission. While immune induction, secretion of antimicrobials and metabolic competition are three typical mechanisms of microbiota-mediated protection against invasive pathogens in mammals, the involvement of metabolic competition or mutualism in mosquito-microbiota and microbiota-<i>Plasmodium</i> interactions has not been investigated. Here, we describe a metabolome analysis of the midgut of <i>Anopheles coluzzii</i> provided with a sugar-meal or a non-infectious blood-meal, under conventional or antibiotic-treated conditions. We observed that the antibiotic treatment affects the tricarboxylic acid cycle and nitrogen metabolism, notably resulting in decreased abundance of free amino acids. Linking our results with published data, we identified pathways which may participate in microbiota-<i>Plasmodium</i> interactions via metabolic interactions or immune modulation and thus would be interesting candidates for future functional studies.https://www.mdpi.com/2076-0817/9/9/679mosquitomicrobiotamalariametabolismimmunitytricarboxylic acid cycle |
| spellingShingle | Estelle Chabanol Volker Behrends Ghislaine Prévot George K. Christophides Mathilde Gendrin Antibiotic Treatment in <i>Anopheles coluzzii</i> Affects Carbon and Nitrogen Metabolism Pathogens mosquito microbiota malaria metabolism immunity tricarboxylic acid cycle |
| title | Antibiotic Treatment in <i>Anopheles coluzzii</i> Affects Carbon and Nitrogen Metabolism |
| title_full | Antibiotic Treatment in <i>Anopheles coluzzii</i> Affects Carbon and Nitrogen Metabolism |
| title_fullStr | Antibiotic Treatment in <i>Anopheles coluzzii</i> Affects Carbon and Nitrogen Metabolism |
| title_full_unstemmed | Antibiotic Treatment in <i>Anopheles coluzzii</i> Affects Carbon and Nitrogen Metabolism |
| title_short | Antibiotic Treatment in <i>Anopheles coluzzii</i> Affects Carbon and Nitrogen Metabolism |
| title_sort | antibiotic treatment in i anopheles coluzzii i affects carbon and nitrogen metabolism |
| topic | mosquito microbiota malaria metabolism immunity tricarboxylic acid cycle |
| url | https://www.mdpi.com/2076-0817/9/9/679 |
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