Responses of Cells to Flow in Vitro
The response of cells to a flow has been studied in vitro. The response of cells was examined in two types of flow channels: a circumnutating flow in a donut-shaped open channel in a culture dish, and a one-way flow in a parallelepiped rhombus flow channel. Variation was made on the material of the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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International Institute of Informatics and Cybernetics
2013-06-01
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| Series: | Journal of Systemics, Cybernetics and Informatics |
| Subjects: | |
| Online Access: | http://www.iiisci.org/Journal/CV$/sci/pdfs/IBA746WE.pdf
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| _version_ | 1811213205065695232 |
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| author | Shigehiro Hashimoto Fumihiko Sato Haruka Hino Hiromichi Fujie Haruka Iwata Yuma Sakatani |
| author_facet | Shigehiro Hashimoto Fumihiko Sato Haruka Hino Hiromichi Fujie Haruka Iwata Yuma Sakatani |
| author_sort | Shigehiro Hashimoto |
| collection | DOAJ |
| description | The response of cells to a flow has been studied in vitro. The response of cells was examined in two types of flow channels: a circumnutating flow in a donut-shaped open channel in a culture dish, and a one-way flow in a parallelepiped rhombus flow channel. Variation was made on the material of the parallelepiped channel to study on adhesion of cells to the plates: glass and polydimethylsiloxane. Behavior of cells on the plate was observed under a flow of a medium with an inverted phase-contrast-microscope. The shear stress on the plate is calculated with an estimated parabolic distribution of the velocity between the parallel plates. The adhesion of cells was evaluated with the cumulated shear, which is a product of the shear stress and the exposure time. The experimental results show that cells are responsive to the flow, which governs orientation, exfoliation, and differentiation. The response depends on the kinds of cells: endothelial cells orient along the stream line, although myocytes orient perpendicular to the stream line. The adhesion depends on the combination between scaffold and cell: myocytes are more adhesive to glass than cartilage cells, and fibroblasts are more adhesive to oxygenated polydimethylsiloxane than glass. |
| first_indexed | 2024-04-12T05:42:03Z |
| format | Article |
| id | doaj.art-516bea43ff784bc7b0bbc2e06c75392a |
| institution | Directory Open Access Journal |
| issn | 1690-4524 |
| language | English |
| last_indexed | 2024-04-12T05:42:03Z |
| publishDate | 2013-06-01 |
| publisher | International Institute of Informatics and Cybernetics |
| record_format | Article |
| series | Journal of Systemics, Cybernetics and Informatics |
| spelling | doaj.art-516bea43ff784bc7b0bbc2e06c75392a2022-12-22T03:45:35ZengInternational Institute of Informatics and CyberneticsJournal of Systemics, Cybernetics and Informatics1690-45242013-06-011152027Responses of Cells to Flow in VitroShigehiro Hashimoto0Fumihiko Sato1Haruka Hino2Hiromichi Fujie3Haruka Iwata4Yuma Sakatani5 Kogakuin University Kogakuin University Kogakuin University Tokyo Metropolitan University Osaka Institute of Technology Osaka Institute of Technology The response of cells to a flow has been studied in vitro. The response of cells was examined in two types of flow channels: a circumnutating flow in a donut-shaped open channel in a culture dish, and a one-way flow in a parallelepiped rhombus flow channel. Variation was made on the material of the parallelepiped channel to study on adhesion of cells to the plates: glass and polydimethylsiloxane. Behavior of cells on the plate was observed under a flow of a medium with an inverted phase-contrast-microscope. The shear stress on the plate is calculated with an estimated parabolic distribution of the velocity between the parallel plates. The adhesion of cells was evaluated with the cumulated shear, which is a product of the shear stress and the exposure time. The experimental results show that cells are responsive to the flow, which governs orientation, exfoliation, and differentiation. The response depends on the kinds of cells: endothelial cells orient along the stream line, although myocytes orient perpendicular to the stream line. The adhesion depends on the combination between scaffold and cell: myocytes are more adhesive to glass than cartilage cells, and fibroblasts are more adhesive to oxygenated polydimethylsiloxane than glass.http://www.iiisci.org/Journal/CV$/sci/pdfs/IBA746WE.pdf OrientationCell CultureBiomedical EngineeringAdhesionShear StressFlow |
| spellingShingle | Shigehiro Hashimoto Fumihiko Sato Haruka Hino Hiromichi Fujie Haruka Iwata Yuma Sakatani Responses of Cells to Flow in Vitro Journal of Systemics, Cybernetics and Informatics Orientation Cell Culture Biomedical Engineering Adhesion Shear Stress Flow |
| title | Responses of Cells to Flow in Vitro |
| title_full | Responses of Cells to Flow in Vitro |
| title_fullStr | Responses of Cells to Flow in Vitro |
| title_full_unstemmed | Responses of Cells to Flow in Vitro |
| title_short | Responses of Cells to Flow in Vitro |
| title_sort | responses of cells to flow in vitro |
| topic | Orientation Cell Culture Biomedical Engineering Adhesion Shear Stress Flow |
| url | http://www.iiisci.org/Journal/CV$/sci/pdfs/IBA746WE.pdf
|
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